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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
08/09/2014 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LABATE, M. T. V.; Ko, KENTON; Ko, Z. W.; PINTO, L. S. R. C.; REAL, M. J. U. D.; ROMANO, M. R.; BARJA, P. R.; GRANELL, A.; FRISO, G.; WIJK, K. J. V.; BRUGNOLI, E.; LABATE, C. A. |
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Afiliação: |
MÔNICA T. V. LABATE, ESALQ; KENTON KO, Queen’s University; ZDENKA W. Ko, Queen’s University; LUCIANA S. R. COSTA PINTO, ESALQ; MARIA J. U. D. REAL, ESALQ; MARCELO RIBEIRO ROMANO, CNPMF; PAULO ROXO BARJA, ESALQ; ANTONIO GRANELL, Universidad Politecnica de Valencia; GIULIA FRISO, Cornell University; KLAAS J. VAN WIJK, Cornell University; ENRICO BRUGNOLI, Instituto di Biologia Agroambientale; CARLOS A. LABATE, ESALQ. |
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Título: |
Constitutive expression of pea Lhcb1-2 in tobacco affects plant development, morphology and photosynthetic capacity. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plant Molecular Biology, v. 55, p. 701-714, 2014. |
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ISSN: |
0167-4412 |
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Idioma: |
Inglês |
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Conteúdo: |
Lhcb1-2 from pea was constitutively expressed in transgenic tobacco plants and assessed for functional impact. The successful assembly of the encoded proteins into LHCII trimers was confirmed by electrospray tandem mass spectrometry. Constitutive production of LHCb1-2 led to increased number of thylakoid membranes per chloroplast, increased grana stacking, higher chloroplast numbers per palisade cell and increased photosynthetic capacity at low irradiance, both on a chlorophyll and leaf area basis. The transgenic plants also displayed increased cell volume, larger leaves, higher leaf number per plant at flowering, increased biomass and increased seed weight, when grown under low irradiance levels. Under high irradiance, both transgenic and wild type plants displayed similar photosynthetic rates when tested at 25 C; however, the non-photochemical quenching (NPQ) and qE values increased in the transgenic plants. The exposure of transgenic plants to a photoinhibitory treatment (4 C for 4 h, under continuous illumination) resulted in more detrimental impairment of photosynthesis, since recovery was slower than the nontransgenic plants. These data indicate that constitutive expression of additional Lhcb1-2 transgenes led to a series of changes at all levels of the plant (cellular, leaf and whole organism), and a delay in flowering and senescence. The additional production of the pea protein appears to be accommodated by increasing cellular structures such as the number of thylakoids per chloroplast, organelle volume, organelles per cell, and leaf expansion. The presence of the trimeric pea protein in the tobacco LHCII, however, caused a possible change in the organization of the associated super-complex, that in turn limited photosynthesis at low temperature MenosLhcb1-2 from pea was constitutively expressed in transgenic tobacco plants and assessed for functional impact. The successful assembly of the encoded proteins into LHCII trimers was confirmed by electrospray tandem mass spectrometry. Constitutive production of LHCb1-2 led to increased number of thylakoid membranes per chloroplast, increased grana stacking, higher chloroplast numbers per palisade cell and increased photosynthetic capacity at low irradiance, both on a chlorophyll and leaf area basis. The transgenic plants also displayed increased cell volume, larger leaves, higher leaf number per plant at flowering, increased biomass and increased seed weight, when grown under low irradiance levels. Under high irradiance, both transgenic and wild type plants displayed similar photosynthetic rates when tested at 25 C; however, the non-photochemical quenching (NPQ) and qE values increased in the transgenic plants. The exposure of transgenic plants to a photoinhibitory treatment (4 C for 4 h, under continuous illumination) resulted in more detrimental impairment of photosynthesis, since recovery was slower than the nontransgenic plants. These data indicate that constitutive expression of additional Lhcb1-2 transgenes led to a series of changes at all levels of the plant (cellular, leaf and whole organism), and a delay in flowering and senescence. The additional production of the pea protein appears to be accommodated by increasing cellular structures such as the number of thylako... Mostrar Tudo |
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Palavras-Chave: |
Tabaco. |
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Thesagro: |
Clorofila; Ervilha; Fumo. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02643naa a2200313 a 4500
001 1994384
005 2023-05-25
008 2014 bl uuuu u00u1 u #d
022 $a0167-4412
100 1 $aLABATE, M. T. V.
245 $aConstitutive expression of pea Lhcb1-2 in tobacco affects plant development, morphology and photosynthetic capacity.$h[electronic resource]
260 $c2014
520 $aLhcb1-2 from pea was constitutively expressed in transgenic tobacco plants and assessed for functional impact. The successful assembly of the encoded proteins into LHCII trimers was confirmed by electrospray tandem mass spectrometry. Constitutive production of LHCb1-2 led to increased number of thylakoid membranes per chloroplast, increased grana stacking, higher chloroplast numbers per palisade cell and increased photosynthetic capacity at low irradiance, both on a chlorophyll and leaf area basis. The transgenic plants also displayed increased cell volume, larger leaves, higher leaf number per plant at flowering, increased biomass and increased seed weight, when grown under low irradiance levels. Under high irradiance, both transgenic and wild type plants displayed similar photosynthetic rates when tested at 25 C; however, the non-photochemical quenching (NPQ) and qE values increased in the transgenic plants. The exposure of transgenic plants to a photoinhibitory treatment (4 C for 4 h, under continuous illumination) resulted in more detrimental impairment of photosynthesis, since recovery was slower than the nontransgenic plants. These data indicate that constitutive expression of additional Lhcb1-2 transgenes led to a series of changes at all levels of the plant (cellular, leaf and whole organism), and a delay in flowering and senescence. The additional production of the pea protein appears to be accommodated by increasing cellular structures such as the number of thylakoids per chloroplast, organelle volume, organelles per cell, and leaf expansion. The presence of the trimeric pea protein in the tobacco LHCII, however, caused a possible change in the organization of the associated super-complex, that in turn limited photosynthesis at low temperature
650 $aClorofila
650 $aErvilha
650 $aFumo
653 $aTabaco
700 1 $aKo, KENTON
700 1 $aKo, Z. W.
700 1 $aPINTO, L. S. R. C.
700 1 $aREAL, M. J. U. D.
700 1 $aROMANO, M. R.
700 1 $aBARJA, P. R.
700 1 $aGRANELL, A.
700 1 $aFRISO, G.
700 1 $aWIJK, K. J. V.
700 1 $aBRUGNOLI, E.
700 1 $aLABATE, C. A.
773 $tPlant Molecular Biology$gv. 55, p. 701-714, 2014.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 26 | |
| 3. |  | BORGES, W. L.; NOVAIS, C. B. de; FARIA, S. M. de. Diversidade de fungos micorrízicos arbusculares em áreas revegetadas após a mineração de Bauxita In: REUNIÃO BRASILEIRA DE CIÊNCIA DO SOLO E NUTRIÇÃO DE PLANTAS, 30.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 14.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 12.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 9.; SIMPÓSIO SOBRE SELÊNIO NO BRASIL, 1., 2012, Maceió. A responsabilidade socioambiental da pesquisa agrícola: anais. Viçosa, MG: SBCS, 2012. FERTBIO 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 4. | | BORGES, W. L.; NOVAIS, C. B. de; FARIA, S. M. de. Diversidade de fungos micorrízicos arbusculares em áreas revegetadas após a mineração de bauxita. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 30.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 14.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 12.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 9.; SIMPÓSIO SOBRE SELÊNIO NO BRASIL, 1., 2012, Maceió. A responsabilidade socioambiental da pesquisa agrícola: anais. Viçosa, AL: SBCS, 2012. 1 CD-ROM. Fertbio. Organizado por Leila Cruz da Silva, Gilson Moura Filho, Adriano Barboza Moura, Abel Washington de Albuquerque.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amapá. |
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| 11. | | NOVAIS, C. R.; DELLA MODESTA, R. C.; FURTADO, A. A. L.; FERREIRA, J. C. S.; LEAL, P. L. S. Avaliação da qualidade sensorial-instrumental de patê de carne de rã (Rana catesbeiana) durante o armazenamento. In: ENCONTRO REGIONAL SUL DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 9., 2007, Curitiba. Inovação, sustentabilidade e competitividade em alimentos: anais... Curitiba: SBCTA: UFPR: UEL, 2007. p. 717-722.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 12. | | NOVAIS, C. B. de; AVIO, L.; GIOVANNETTI, M.; FARIA, S. M. de; SIQUEIRA, J. O.; SBRANAE, C. Interconnectedness, length and viability of arbuscular mycorrhizal mycelium as affected by selected herbicides and fungicides. Applied Soil Ecology, v. 143, p. 144-152, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 15. | | BENTO, R. A.; NOVAIS, C. B. de; SAGGIN JUNIOR, O. J.; OLIVEIRA, L. A. de; SAMPAIO, P. de T. B. Pioneer Tree Bellucia imperialis (Melastomataceae) from Central Amazon with seedlings highly dependent on arbuscular mycorrhizal fungi. Journal of Fungi, , v. 9, n. 5, 540, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 17. | | SAGGIN JÚNIOR, O. J.; NOVAIS, C. B. de; SILVA, E. M. R. da; DE SOUZA, F. A. Sistematização e conservação da coleção de fungos micorrízicos arbusculares da Embrapa Agrobiologia. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 26., REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 10., SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 8., REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 5., 2004, Lajes, SC. [avaliação das conquistas: bases para estratégias futuras: anais]. Lages: SBCS; UDESC Lages, Departamento de Solos, 2004. FERTBIO 2004. 4 p. 1 CD-ROM.| Biblioteca(s): Embrapa Agrobiologia. |
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| 18. | | BORGES, W. L.; NOVAIS, C. B.; SAGGIN JUNIOR, O. J.; PRIN, Y.; LE ROUX, C.; DUCOUSSO, M.; FARIA, S. M. de. Arbuscular mycorrhizal fungi diversity in revegetated areas after bauxite mining. African Journal of Microbiology Research, Sapele, v. 8, n. 28, p. 2733-2738, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agrobiologia; Embrapa Amapá. |
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| 19. | | OLIVEIRA, J. R. de; SAGGIN-JÚNIOR, O. J.; NOVAIS, C. B. de; LIMA, W. L. de; AQUINO, A. M. de; SILVA, E. M. R. da. Avaliação de esporos de fungos micorrízicos arbusculares ingeridos por minhocas. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 30., 2005, Recife. Solos: sustentabilidade e qualidade ambiental. Recife: Sociedade Brasileira de Ciência do Solo, 2005. 4 p. 1 CD-ROM.| Biblioteca(s): Embrapa Agrobiologia. |
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| 20. | | BRAGA, J. G. B.; NOVAIS, C. B. de; DINIZ, P. P.; ARAGÃO, O. O. da S.; SAGGIN JUNIOR, O. J.; JESUS, E. da C. Association of mycoheterotrophic Gentianaceae with specific Glomus lineages. Mycorrhiza, v. 33, n. 4, p. 249 - 256, July 2023.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 26 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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