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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com rosangela.lacerda@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
04/09/2014 |
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Data da última atualização: |
23/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GUIMARAES, C. T.; SIMOES, C. C.; PASTINA, M. M.; MARON, L. G.; MAGALHAES, J. V.; VASCONCELLOS, R. C. C.; GUIMARAES, L. J. M.; LANA, U. G. de P.; TINOCO, C. F. S.; NODA, R. W.; BELICUAS, S. N. J.; KOCHIAN, L. V.; ALVES, V. M. C.; PARENTONI, S. N. |
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Afiliação: |
CLAUDIA TEIXEIRA GUIMARAES, CNPMS; MARIA MARTA PASTINA, CNPMS; JURANDIR VIEIRA DE MAGALHAES, CNPMS; LAURO JOSE MOREIRA GUIMARAES, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS; ROBERTO WILLIANS NODA, CNPMS; SILVIA NETO JARDIM BELICUAS, CNPMS; VERA MARIA CARVALHO ALVES, CNPMS; SIDNEY NETTO PARENTONI, CNPMS. |
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Título: |
Genetic dissection of Al tolerance QTLs in the maize genome by high density SNP scan. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
BMC Genomics, v. 15, n. 153, p. 1-14, 2014. |
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DOI: |
10.1186/1471-2164-15-153 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Aluminum (Al) toxicity is an important limitation to food security in tropical and subtropical regions.High Al saturation on acid soils limits root development, reducing water and nutrient uptake. In addition to naturally occurring acid soils, agricultural practices may decrease soil pH, leading to yield losses due to Al toxicity. Elucidating the genetic and molecular mechanisms underlying maize Al tolerance is expected to accelerate the development of Al-tolerant cultivars. Results: Five genomic regions were significantly associated with Al tolerance, using 54,455 SNP markers in are combinant inbred line population derived from Cateto Al237. Candidate genes co-localized with Al tolerance QTLs were further investigated. Near-isogenic lines (NILs) developed for ZmMATE2 were as Al-sensitive as the recurrent line, indicating that this candidate gene was not responsible for the Al tolerance QTL on chromosome 5, qALT5. However, ZmNrat1, a maize homolog to OsNrat1, which encodes an Al3+ specific transporter previously implicated in rice Al tolerance, was mapped at ~40 Mbp from qALT5. We demonstrate for the first time that ZmNrat1 is preferentially expressed in maize root tips and is up-regulated by Al, similarly to OsNrat1 in rice, suggesting a role of this gene in maize Al tolerance. The strongest-effect QTL was mapped on chromosome 6 (qALT6), within a 0.5 Mbp region where three copies of the Al tolerance gene, ZmMATE1, were found in tandem configuration. qALT6 was shown to increase Al tolerance in maize; the qALT6-NILs carrying three copies of ZmMATE1 exhibited a two-fold increase in Al tolerance, and higher expression of ZmMATE1 compared to the Al sensitive recurrent parent. Interestingly, a new source of Al tolerance via ZmMATE1 was identified in a Brazilian elite line that showed high expression of ZmMATE1 but carries a single copy of ZmMATE1. Conclusions: High ZmMATE1 expression, controlled either by three copies of the target gene or by an unknown molecular mechanism, is responsible for Al tolerance mediated by qALT6 . As Al tolerant alleles at qALT6 are rare in maize, marker-assisted introgression of this QTL is an important strategy to improve maize adaptation to acid soils worldwide. MenosBackground: Aluminum (Al) toxicity is an important limitation to food security in tropical and subtropical regions.High Al saturation on acid soils limits root development, reducing water and nutrient uptake. In addition to naturally occurring acid soils, agricultural practices may decrease soil pH, leading to yield losses due to Al toxicity. Elucidating the genetic and molecular mechanisms underlying maize Al tolerance is expected to accelerate the development of Al-tolerant cultivars. Results: Five genomic regions were significantly associated with Al tolerance, using 54,455 SNP markers in are combinant inbred line population derived from Cateto Al237. Candidate genes co-localized with Al tolerance QTLs were further investigated. Near-isogenic lines (NILs) developed for ZmMATE2 were as Al-sensitive as the recurrent line, indicating that this candidate gene was not responsible for the Al tolerance QTL on chromosome 5, qALT5. However, ZmNrat1, a maize homolog to OsNrat1, which encodes an Al3+ specific transporter previously implicated in rice Al tolerance, was mapped at ~40 Mbp from qALT5. We demonstrate for the first time that ZmNrat1 is preferentially expressed in maize root tips and is up-regulated by Al, similarly to OsNrat1 in rice, suggesting a role of this gene in maize Al tolerance. The strongest-effect QTL was mapped on chromosome 6 (qALT6), within a 0.5 Mbp region where three copies of the Al tolerance gene, ZmMATE1, were found in tandem configuration. qALT6 was sh... Mostrar Tudo |
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Palavras-Chave: |
Genotipagem; Sequenciamento. |
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Thesagro: |
Genética; Mate; Milho; Zea mays. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03239naa a2200361 a 4500
001 1994210
005 2017-05-23
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1186/1471-2164-15-153$2DOI
100 1 $aGUIMARAES, C. T.
245 $aGenetic dissection of Al tolerance QTLs in the maize genome by high density SNP scan.$h[electronic resource]
260 $c2014
520 $aBackground: Aluminum (Al) toxicity is an important limitation to food security in tropical and subtropical regions.High Al saturation on acid soils limits root development, reducing water and nutrient uptake. In addition to naturally occurring acid soils, agricultural practices may decrease soil pH, leading to yield losses due to Al toxicity. Elucidating the genetic and molecular mechanisms underlying maize Al tolerance is expected to accelerate the development of Al-tolerant cultivars. Results: Five genomic regions were significantly associated with Al tolerance, using 54,455 SNP markers in are combinant inbred line population derived from Cateto Al237. Candidate genes co-localized with Al tolerance QTLs were further investigated. Near-isogenic lines (NILs) developed for ZmMATE2 were as Al-sensitive as the recurrent line, indicating that this candidate gene was not responsible for the Al tolerance QTL on chromosome 5, qALT5. However, ZmNrat1, a maize homolog to OsNrat1, which encodes an Al3+ specific transporter previously implicated in rice Al tolerance, was mapped at ~40 Mbp from qALT5. We demonstrate for the first time that ZmNrat1 is preferentially expressed in maize root tips and is up-regulated by Al, similarly to OsNrat1 in rice, suggesting a role of this gene in maize Al tolerance. The strongest-effect QTL was mapped on chromosome 6 (qALT6), within a 0.5 Mbp region where three copies of the Al tolerance gene, ZmMATE1, were found in tandem configuration. qALT6 was shown to increase Al tolerance in maize; the qALT6-NILs carrying three copies of ZmMATE1 exhibited a two-fold increase in Al tolerance, and higher expression of ZmMATE1 compared to the Al sensitive recurrent parent. Interestingly, a new source of Al tolerance via ZmMATE1 was identified in a Brazilian elite line that showed high expression of ZmMATE1 but carries a single copy of ZmMATE1. Conclusions: High ZmMATE1 expression, controlled either by three copies of the target gene or by an unknown molecular mechanism, is responsible for Al tolerance mediated by qALT6 . As Al tolerant alleles at qALT6 are rare in maize, marker-assisted introgression of this QTL is an important strategy to improve maize adaptation to acid soils worldwide.
650 $aGenética
650 $aMate
650 $aMilho
650 $aZea mays
653 $aGenotipagem
653 $aSequenciamento
700 1 $aSIMOES, C. C.
700 1 $aPASTINA, M. M.
700 1 $aMARON, L. G.
700 1 $aMAGALHAES, J. V.
700 1 $aVASCONCELLOS, R. C. C.
700 1 $aGUIMARAES, L. J. M.
700 1 $aLANA, U. G. de P.
700 1 $aTINOCO, C. F. S.
700 1 $aNODA, R. W.
700 1 $aBELICUAS, S. N. J.
700 1 $aKOCHIAN, L. V.
700 1 $aALVES, V. M. C.
700 1 $aPARENTONI, S. N.
773 $tBMC Genomics$gv. 15, n. 153, p. 1-14, 2014.
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