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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
01/09/2014 |
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Data da última atualização: |
09/12/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MÜLLER, B. S. de F.; SAKAMOTO, T.; MENEZES, I. P. P. de; PRADO, G. S.; MARTINS, W. S.; BRONDANI, C.; BARROS, E. G. de; VIANELLO, R. P. |
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Afiliação: |
BÁRBARA SALOMÃO DE FARIA MÜLLER, UFV; TETSU SAKAMOTO, UFMG; IVANDILSON PESSOA PINTO DE MENEZES, INSTITUTO FEDERAL GOIANO, Urutaí-GO; GUILHERME SOUZA PRADO, bolsista CNPAF; WELLINGTON SANTOS MARTINS, UFG; CLAUDIO BRONDANI, CNPAF; EVERALDO GONÇALVES DE BARROS, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ROSANA PEREIRA VIANELLO, CNPAF. |
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Título: |
Analysis of BAC-end sequences in common bean (Phaseolus vulgaris L.) towards the development and characterization of long motifs SSRs. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plant Molecular Biology, Dordrecht, v. 86, n. 4/5, p. 455-470, Nov. 2014. |
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DOI: |
10.1007/s11103-014-0240-7 |
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Idioma: |
Inglês |
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Conteúdo: |
The increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number of bacterial artificial chromosome (BAC) were sequenced, annotated and the presence of repetitive sequences was determined. In total, 52,270 BESs (BAC-end sequences), equivalent to 32 Mbp (~6 %) of the genome, were processed. In total, 3,789 BES-SSRs were identified, with a distribution of one SSR (simple sequence repeat) per 8.36 kbp and 2,000 were suitable for the development of SSRs, of which 194 were evaluated in low-resolution screening. From 40 BES-SSRs based on long motifs SSRs (≥trinucleotides) analyzed in high-resolution genotyping, 34 showed an equally good amplification for the Andean and for the Mesoamerican genepools, exhibiting an average gene diversity (H E) of 0.490 and 5.59 alleles/locus, of which six classified as Class I showed a H E ≥ 0.7. The PCoA and structure analysis allowed to discriminate the gene pools (K = 2, FST = 0.733). From the 52,270 BESs, 2 % corresponded to transcription factors and 3 % to transposable elements. Putative functions for 24,321 BESs were identified and for 19,363 were assigned functional categories (gene ontology). This study identified highly polymorphic BES-SSRs containing tri- to hexanucleotides motifs and bringing together relevant genetic characteristics useful for breeding programs. Additionally, the BESs were incorporated into the international genome-sequencing project for the common bean. MenosThe increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number of bacterial artificial chromosome (BAC) were sequenced, annotated and the presence of repetitive sequences was determined. In total, 52,270 BESs (BAC-end sequences), equivalent to 32 Mbp (~6 %) of the genome, were processed. In total, 3,789 BES-SSRs were identified, with a distribution of one SSR (simple sequence repeat) per 8.36 kbp and 2,000 were suitable for the development of SSRs, of which 194 were evaluated in low-resolution screening. From 40 BES-SSRs based on long motifs SSRs (≥trinucleotides) analyzed in high-resolution genotyping, 34 showed an equally good amplification for the Andean and for the Mesoamerican genepools, exhibiting an average gene diversity (H E) of 0.490 and 5.59 alleles/locus, of which six classified as Class I showed a H E ≥ 0.7. The PCoA and structure analysis allowed to discriminate the gene pools (K = 2, FST = 0.733). From the 52,270 BESs, 2 % corresponded to transcription factors and 3 % to transposable elements. Putative functions for 24,321 BESs were identified and for 19,363 were assigned functional categories (gene ontology). This study identified highly polymorphic BES-SSRs containing tri- to hexanucleotides mo... Mostrar Tudo |
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Thesagro: |
Feijão; Genética molecular; Phaseolus vulgaris. |
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Thesaurus Nal: |
Beans; Fabaceae; Genomics; Microsatellite repeats; Molecular genetics; Transcription factors; Transposons. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02734naa a2200337 a 4500
001 1993888
005 2014-12-09
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1007/s11103-014-0240-7$2DOI
100 1 $aMÜLLER, B. S. de F.
245 $aAnalysis of BAC-end sequences in common bean (Phaseolus vulgaris L.) towards the development and characterization of long motifs SSRs.$h[electronic resource]
260 $c2014
520 $aThe increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number of bacterial artificial chromosome (BAC) were sequenced, annotated and the presence of repetitive sequences was determined. In total, 52,270 BESs (BAC-end sequences), equivalent to 32 Mbp (~6 %) of the genome, were processed. In total, 3,789 BES-SSRs were identified, with a distribution of one SSR (simple sequence repeat) per 8.36 kbp and 2,000 were suitable for the development of SSRs, of which 194 were evaluated in low-resolution screening. From 40 BES-SSRs based on long motifs SSRs (≥trinucleotides) analyzed in high-resolution genotyping, 34 showed an equally good amplification for the Andean and for the Mesoamerican genepools, exhibiting an average gene diversity (H E) of 0.490 and 5.59 alleles/locus, of which six classified as Class I showed a H E ≥ 0.7. The PCoA and structure analysis allowed to discriminate the gene pools (K = 2, FST = 0.733). From the 52,270 BESs, 2 % corresponded to transcription factors and 3 % to transposable elements. Putative functions for 24,321 BESs were identified and for 19,363 were assigned functional categories (gene ontology). This study identified highly polymorphic BES-SSRs containing tri- to hexanucleotides motifs and bringing together relevant genetic characteristics useful for breeding programs. Additionally, the BESs were incorporated into the international genome-sequencing project for the common bean.
650 $aBeans
650 $aFabaceae
650 $aGenomics
650 $aMicrosatellite repeats
650 $aMolecular genetics
650 $aTranscription factors
650 $aTransposons
650 $aFeijão
650 $aGenética molecular
650 $aPhaseolus vulgaris
700 1 $aSAKAMOTO, T.
700 1 $aMENEZES, I. P. P. de
700 1 $aPRADO, G. S.
700 1 $aMARTINS, W. S.
700 1 $aBRONDANI, C.
700 1 $aBARROS, E. G. de
700 1 $aVIANELLO, R. P.
773 $tPlant Molecular Biology, Dordrecht$gv. 86, n. 4/5, p. 455-470, Nov. 2014.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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