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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
31/03/2014 |
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Data da última atualização: |
26/05/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MASCARIN, G. M.; KOBORI, N. N.; VITAL, R. C. de J.; JACKSON, M. A.; QUINTELA, E. D. |
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Afiliação: |
GABRIEL MOURA MASCARIN, CNPAF; NILCE NAOMI KOBORI; RAYAN CARLOS DE JESUS VITAL; MARK ALAN JACKSON, USDA; ELIANE DIAS QUINTELA, CNPAF. |
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Título: |
Production of microsclerotia by Brazilian strains of Metarhizium spp. using submerged liquid culture fermentation. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
World Journal of Microbiology & Biotechnology, Oxford, v. 30, n. 5, p. 1583-1590, May 2014. |
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DOI: |
DOI 10.1007/s11274-013-1581-0 |
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Idioma: |
Inglês |
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Conteúdo: |
We investigated the potential production and desiccation tolerance of microsclerotia (MS) by Brazilian strains of Metarhizium anisopliae (Ma), M. acridum (Mc) and M. robertsii (Mr). These fungi were grown in a liquid medium containing 16 g carbon l-1 with a carbon:nitrogen ratio of 50:1. One hundred milliliters cultures were grown in 250 ml Erlenmeyer flasks in a rotary incubator shaker at 28 C and 200 rpm for 5 days. Five-day-old MS were harvested, mixed with diatomaceous earth (DE) and airdried for 2 days at 30 C. The air-dried MS?DE granular preparations were milled by mortar ? pestle and stored in centrifuged tubes at either 26 or -20 C. Desiccation tolerance and conidia production were assessed for dried MS granules by measuring hyphal germination after incubation for 2 days on water agar plates at 26 C and for conidia production following 7 days incubation. Yields of MS by all strains of Metarhizium were 6.1?7.3 9 106 l-1 after 3 days growth with maximum MS yields (0.7?1.1 9 107 l-1) after 5 days growth. No differences in biomass accumulation were observed after 3 days growth, whereas Ma- CG168 showed the highest biomass accumulation after 5 days growth. Dried MS?DE preparations of all fungal strains were equally tolerant to desiccation (C93 % germination) and the highest conidia production was obtained by MS granules of Mc-CG423 (4 9 109 conidia g-1). All MS granules showed similar stability after storage at either 26 or -20 C for 3.5 months. |
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Thesagro: |
Controle biológico; Metarhizium anisopliae. |
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Thesaurus Nal: |
Ascomycota; Biological control; Drying; Mycoinsecticides. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
LEADER 02299naa a2200253 a 4500
001 1983676
005 2014-05-26
008 2014 bl uuuu u00u1 u #d
024 7 $aDOI 10.1007/s11274-013-1581-0$2DOI
100 1 $aMASCARIN, G. M.
245 $aProduction of microsclerotia by Brazilian strains of Metarhizium spp. using submerged liquid culture fermentation.$h[electronic resource]
260 $c2014
520 $aWe investigated the potential production and desiccation tolerance of microsclerotia (MS) by Brazilian strains of Metarhizium anisopliae (Ma), M. acridum (Mc) and M. robertsii (Mr). These fungi were grown in a liquid medium containing 16 g carbon l-1 with a carbon:nitrogen ratio of 50:1. One hundred milliliters cultures were grown in 250 ml Erlenmeyer flasks in a rotary incubator shaker at 28 C and 200 rpm for 5 days. Five-day-old MS were harvested, mixed with diatomaceous earth (DE) and airdried for 2 days at 30 C. The air-dried MS?DE granular preparations were milled by mortar ? pestle and stored in centrifuged tubes at either 26 or -20 C. Desiccation tolerance and conidia production were assessed for dried MS granules by measuring hyphal germination after incubation for 2 days on water agar plates at 26 C and for conidia production following 7 days incubation. Yields of MS by all strains of Metarhizium were 6.1?7.3 9 106 l-1 after 3 days growth with maximum MS yields (0.7?1.1 9 107 l-1) after 5 days growth. No differences in biomass accumulation were observed after 3 days growth, whereas Ma- CG168 showed the highest biomass accumulation after 5 days growth. Dried MS?DE preparations of all fungal strains were equally tolerant to desiccation (C93 % germination) and the highest conidia production was obtained by MS granules of Mc-CG423 (4 9 109 conidia g-1). All MS granules showed similar stability after storage at either 26 or -20 C for 3.5 months.
650 $aAscomycota
650 $aBiological control
650 $aDrying
650 $aMycoinsecticides
650 $aControle biológico
650 $aMetarhizium anisopliae
700 1 $aKOBORI, N. N.
700 1 $aVITAL, R. C. de J.
700 1 $aJACKSON, M. A.
700 1 $aQUINTELA, E. D.
773 $tWorld Journal of Microbiology & Biotechnology, Oxford$gv. 30, n. 5, p. 1583-1590, May 2014.
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| 1. |  | MONFORT, L. F. M.; LAMEIRA, O. A.; MENESES, A. A. S. de. Efeito do ácido indolbutírico (aib) no enraizamento de estacas de pariri (Arrabidaea chica). In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA UFRA, 4.; SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL, 10., 2007, Belém, PA. Anais... Belém, PA: UFRA: Embrapa Amazônia Oriental, 2007. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 2. | | MONFORT, L. F. M.; LAMEIRA, O. A.; MENESES, A. A. S. de; SALDANHA, A. L. M. Efeitos de substratos em mudas de curauá (Ananas erectifolius L. B. Smith). In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA UFRA, 4.; SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL, 10., 2007, Belém, PA. Anais... Belém, PA: UFRA: Embrapa Amazônia Oriental, 2007. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 3. | | MENESES, A. A. S. de; LAMEIRA, O. A.; MONFORT, L. F. M.; SALDANHA, A. L. M. Efeito de substratos na germinação de sementes em genótipos de jaborandi (Pilocarpus microphyllus) Stapf ex Holm. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA UFRA, 4.; SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL, 10., 2007, Belém, PA. Anais... Belém, PA: UFRA: Embrapa Amazônia Oriental, 2007. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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