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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com rosangela.lacerda@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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Data corrente: |
08/10/2013 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SIQUEIRA, L. G. B.; AREAS, V. S.; GHETTI, A. M.; FONSECA, J. F. da; PALHAO, M. P.; FERNANDES, C. A. C.; VIANA, J. H. M. |
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Afiliação: |
LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; V. S. AREAS, UFES; A. M. GHETTI, UFF; JEFERSON FERREIRA DA FONSECA, CNPC; M. P. PALHAO, UJRV, Alfenas, MG; C. A. C. FERNANDES, UJRV, Alfenas MG; JOAO HENRIQUE MOREIRA VIANA, CNPGL. |
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Título: |
Color Doppler flow imaging for the early detection of nonpregnant cattle at 20 days after timed artificial insemination. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 96, n. 10, p. 6461-6472, 2013. |
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DOI: |
https://doi.org/10.3168/jds.2013-6814 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The objective was to determine the accuracy of a pregnancy test for predicting nonpregnant cattle based on the evaluation of corpus luteum (CL) blood flow at 20 d (CLBF-d20) after timed artificial insemination (TAI). Crossbred Holstein-Gir dairy heifers (n = 209) and lactating cows (n = 317) were synchronized for TAI using the following protocol: intravaginal implant (1.0 g of progesterone) and 2 mg of estradiol benzoate i.m. on d −10, implant removal and 0.526 mg of sodium cloprostenol i.m. on d −2, 1 mg of estradiol benzoate i.m. on d −1, and TAI on d 0. On d 20, animals underwent grayscale ultrasonography (US) to locate the CL and color flow Doppler to evaluate CLBF-d20 using a portable ultrasound equipped with a 7.5-MHz rectal transducer. Based only on a visual, subjective CLBF evaluation, the animals were classified as pregnant or not pregnant. On d 30 to 35, blinded from results of the previous diagnosis, the same operator performed a final pregnancy diagnosis using US to visualize the fetal heartbeat (gold standard; US-d30). A second evaluator also analyzed the CLBF-d20 in the same animals by watching 7-s recorded videos. Blood samples were collected from a subset of 171 females to determine, by RIA, plasma progesterone (P4) concentrations, which indicate CL function. The final pregnancy outcome (US-d30) was retrospectively compared with the CLBFd20 diagnoses and then classified either as correct or incorrect. The number of true positive, true negative, false positive, and false negative decisions were inserted into a 2 × 2 decision matrix. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of the CLBF-d20 test were calculated using specific equations. Binomial variables (pregnancy rate and proportions) were analyzed using Fisher?s exact test for the effect of parity and to compare between evaluators and tests (CLBF-d20 vs. plasma P4). The kappa values were calculated to quantify the agreement between CLBF-d20 and the gold standard (US-d30) and between evaluators. The performance parameters of CLBF-d20 test were as follows: sensitivity = 99.0%, specificity = 53.7%, positive predictive value = 65.1%, negative predictive value = 98.5%, and accuracy = 74.8%. False negatives represented only 0.4% of the exams. No differences existed in these parameters between evaluators (no. 1 vs. no. 2) and tests (CLBF-d20 vs. plasma P4). Moreover, a high level of agreement was observed between evaluators (0.91). In conclusion, visual evaluation of CLBF-d20 represents a quick, reliable, and consistent diagnostic test that enables the early detection of nonpregnant cattle. MenosAbstract: The objective was to determine the accuracy of a pregnancy test for predicting nonpregnant cattle based on the evaluation of corpus luteum (CL) blood flow at 20 d (CLBF-d20) after timed artificial insemination (TAI). Crossbred Holstein-Gir dairy heifers (n = 209) and lactating cows (n = 317) were synchronized for TAI using the following protocol: intravaginal implant (1.0 g of progesterone) and 2 mg of estradiol benzoate i.m. on d −10, implant removal and 0.526 mg of sodium cloprostenol i.m. on d −2, 1 mg of estradiol benzoate i.m. on d −1, and TAI on d 0. On d 20, animals underwent grayscale ultrasonography (US) to locate the CL and color flow Doppler to evaluate CLBF-d20 using a portable ultrasound equipped with a 7.5-MHz rectal transducer. Based only on a visual, subjective CLBF evaluation, the animals were classified as pregnant or not pregnant. On d 30 to 35, blinded from results of the previous diagnosis, the same operator performed a final pregnancy diagnosis using US to visualize the fetal heartbeat (gold standard; US-d30). A second evaluator also analyzed the CLBF-d20 in the same animals by watching 7-s recorded videos. Blood samples were collected from a subset of 171 females to determine, by RIA, plasma progesterone (P4) concentrations, which indicate CL function. The final pregnancy outcome (US-d30) was retrospectively compared with the CLBFd20 diagnoses and then classified either as correct or incorrect. The number of true positive, t... Mostrar Tudo |
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Palavras-Chave: |
Bovines; Doppler; Luteólise; Ultra-Som; Ultrassonografia. |
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Thesagro: |
Bovino; Circulação Sangüínea; Corpo lúteo; Diagnóstico; Gado; Inseminação artificial; Prenhez; Reprodução animal; Vaca. |
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Thesaurus Nal: |
Animal reproduction; Blood circulation; blood flow; Cattle; corpus luteum; Luteolysis; Pregnancy. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03964naa a2200457 a 4500
001 1980679
005 2024-02-05
008 2013 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3168/jds.2013-6814$2DOI
100 1 $aSIQUEIRA, L. G. B.
245 $aColor Doppler flow imaging for the early detection of nonpregnant cattle at 20 days after timed artificial insemination.$h[electronic resource]
260 $c2013
520 $aAbstract: The objective was to determine the accuracy of a pregnancy test for predicting nonpregnant cattle based on the evaluation of corpus luteum (CL) blood flow at 20 d (CLBF-d20) after timed artificial insemination (TAI). Crossbred Holstein-Gir dairy heifers (n = 209) and lactating cows (n = 317) were synchronized for TAI using the following protocol: intravaginal implant (1.0 g of progesterone) and 2 mg of estradiol benzoate i.m. on d −10, implant removal and 0.526 mg of sodium cloprostenol i.m. on d −2, 1 mg of estradiol benzoate i.m. on d −1, and TAI on d 0. On d 20, animals underwent grayscale ultrasonography (US) to locate the CL and color flow Doppler to evaluate CLBF-d20 using a portable ultrasound equipped with a 7.5-MHz rectal transducer. Based only on a visual, subjective CLBF evaluation, the animals were classified as pregnant or not pregnant. On d 30 to 35, blinded from results of the previous diagnosis, the same operator performed a final pregnancy diagnosis using US to visualize the fetal heartbeat (gold standard; US-d30). A second evaluator also analyzed the CLBF-d20 in the same animals by watching 7-s recorded videos. Blood samples were collected from a subset of 171 females to determine, by RIA, plasma progesterone (P4) concentrations, which indicate CL function. The final pregnancy outcome (US-d30) was retrospectively compared with the CLBFd20 diagnoses and then classified either as correct or incorrect. The number of true positive, true negative, false positive, and false negative decisions were inserted into a 2 × 2 decision matrix. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of the CLBF-d20 test were calculated using specific equations. Binomial variables (pregnancy rate and proportions) were analyzed using Fisher?s exact test for the effect of parity and to compare between evaluators and tests (CLBF-d20 vs. plasma P4). The kappa values were calculated to quantify the agreement between CLBF-d20 and the gold standard (US-d30) and between evaluators. The performance parameters of CLBF-d20 test were as follows: sensitivity = 99.0%, specificity = 53.7%, positive predictive value = 65.1%, negative predictive value = 98.5%, and accuracy = 74.8%. False negatives represented only 0.4% of the exams. No differences existed in these parameters between evaluators (no. 1 vs. no. 2) and tests (CLBF-d20 vs. plasma P4). Moreover, a high level of agreement was observed between evaluators (0.91). In conclusion, visual evaluation of CLBF-d20 represents a quick, reliable, and consistent diagnostic test that enables the early detection of nonpregnant cattle.
650 $aAnimal reproduction
650 $aBlood circulation
650 $ablood flow
650 $aCattle
650 $acorpus luteum
650 $aLuteolysis
650 $aPregnancy
650 $aBovino
650 $aCirculação Sangüínea
650 $aCorpo lúteo
650 $aDiagnóstico
650 $aGado
650 $aInseminação artificial
650 $aPrenhez
650 $aReprodução animal
650 $aVaca
653 $aBovines
653 $aDoppler
653 $aLuteólise
653 $aUltra-Som
653 $aUltrassonografia
700 1 $aAREAS, V. S.
700 1 $aGHETTI, A. M.
700 1 $aFONSECA, J. F. da
700 1 $aPALHAO, M. P.
700 1 $aFERNANDES, C. A. C.
700 1 $aVIANA, J. H. M.
773 $tJournal of Dairy Science$gv. 96, n. 10, p. 6461-6472, 2013.
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Embrapa Gado de Leite (CNPGL) |
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| 1. |  | GOODWIN, S. B.; M’BAREK, S. B.; DHILLON, B.; WITTENBERG, A. H. J.; CRANE, C. F.; HANE, J. K.; FOSTER, A. J.; LEE, T. A. J. van der; GRIMWOOD, J.; AERTS, A.; ANTONIW, J.; BAILEY, A.; BLUHM, B.; BOWLER, J.; BRISTOW, J.; BURGT, A. van der; CANTO CANCHE, B.; CHURCHILL, A. C. L.; CONDE FERRÀEZ, L.; COOLS, H. J.; COUTINHO, P. M.; CSUKAI, M.; DEHAL, P.; WIT, P. de; DONZELLI, B.; GEEST, H. C. van de; HAM, R. C. H. H. van; HAMMOND KOSACK, K. E.; HENRISSAT, B.; KILIAN, A.; KOBAYASHI, A. K.; KOOPMANN, E.; KOURMPETIS, Y.; KUZNIAR, A.; LINDQUIST, E.; LOMBARD, V.; MALIEPAARD, C.; MARTINS, N. F.; MEHRABI, R.; NAP, J. P. H.; PONOMARENKO, A.; RUDD, J. J.; SALAMOV, A.; SCHMUTZ, J.; SCHOUTEN, H. J.; SHAPIRO, H.; STERGIOPOULOS, I.; TORRIANI, S. F. F.; TU, H.; VRIES, R. P. de; WAALWIJK, C.; WARE, S. B.; WIEBENGA, A.; ZWIERS, L.; OLIVER, R. P.; GRIGORIEV, I. V.; KEMA, G. H. J. Finished genome of the fungal wheat pathogen Mycosphaerella graminicola Reveals dispensome structure, chromosome plasticity, and stealth pathogenesis. Plos Genetics, v. 7, n. 6, e1002070, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Meio-Norte; Embrapa Recursos Genéticos e Biotecnologia. |
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