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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
11/02/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PEREIRA, M. M.; RAPOSO, N. R. B.; BRAYNER, R.; TEIXEIRA, E. M.; OLIVEIRA, V.; QUINTAO, C. C. R.; CAMARGO, L. S. de A.; MATTOSO, L. H. C.; BRANDAO, H. de M. |
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Afiliação: |
M. M. PEREIRA, UFJF; N. R. B. RAPOSO, UFJF; R. BRAYNER, University of Paris Diderot, Paris; E. M. TEIXEIRA; V. OLIVEIRA, UFJF; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA; HUMBERTO DE MELLO BRANDAO, CNPGL. |
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Título: |
Cytotoxicity and expression of genes involved in the cellular stress response and apoptosis in mammalian fibroblast exposed to cotton cellulose nanofibers. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Nanotechnology, v. 24, n. 7, 075103, 2013. |
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DOI: |
https://doi.org/10.1088/0957-4484/24/7/075103 |
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Idioma: |
Inglês |
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Conteúdo: |
Cellulose nanofibers (CNF) have mechanical properties that make them very attractive for applications in the construction of polymeric matrices, drug delivery and tissue engineering. However, little is known about their impact on mammalian cells. The objective of this study was to evaluate the cytotoxicity of CNF and their effect on gene expression of fibroblasts cultured in vitro. The morphology of CNF was analyzed by transmission electron microscopy and the surface charge by Zeta potential. Cell viability was analyzed by flow cytometry assay and gene expression of biomarkers focused on cell stress response such as Heat shock protein 70.1 (HSP70.1) and Peroxiredoxin 1 (PRDX1) and apoptosis as B-cell leukemia (BCL-2) and BCL-2 associated X protein (BAX) by RT-PCR assay. Low concentrations of CNF (0.02-100 μg ml(-1)) did not cause cell death; however, at concentrations above 200 μg ml(-1), the nanofibers significantly decreased cell viability (86.41 ± 5.37%). The exposure to high concentrations of CNF (2000 and 5000 μg ml(-1)) resulted in increased HSP70.1, PRDX1 and BAX gene expression. The current study concludes that, under the conditions tested, high concentrations (2000 and 5000 μg ml(-1)) of CNF cause decreased cell viability and affect the expression of stress- and apoptosis-associated molecular markers. |
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Palavras-Chave: |
Animals Apoptosis; Apoptosis/genetics; Cattle Cellulose/pharmacology; Drug effects; Fibroblasts/cytology; Fibroblasts/drug. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02323naa a2200301 a 4500
001 1979437
005 2024-02-05
008 2013 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1088/0957-4484/24/7/075103$2DOI
100 1 $aPEREIRA, M. M.
245 $aCytotoxicity and expression of genes involved in the cellular stress response and apoptosis in mammalian fibroblast exposed to cotton cellulose nanofibers.$h[electronic resource]
260 $c2013
520 $aCellulose nanofibers (CNF) have mechanical properties that make them very attractive for applications in the construction of polymeric matrices, drug delivery and tissue engineering. However, little is known about their impact on mammalian cells. The objective of this study was to evaluate the cytotoxicity of CNF and their effect on gene expression of fibroblasts cultured in vitro. The morphology of CNF was analyzed by transmission electron microscopy and the surface charge by Zeta potential. Cell viability was analyzed by flow cytometry assay and gene expression of biomarkers focused on cell stress response such as Heat shock protein 70.1 (HSP70.1) and Peroxiredoxin 1 (PRDX1) and apoptosis as B-cell leukemia (BCL-2) and BCL-2 associated X protein (BAX) by RT-PCR assay. Low concentrations of CNF (0.02-100 μg ml(-1)) did not cause cell death; however, at concentrations above 200 μg ml(-1), the nanofibers significantly decreased cell viability (86.41 ± 5.37%). The exposure to high concentrations of CNF (2000 and 5000 μg ml(-1)) resulted in increased HSP70.1, PRDX1 and BAX gene expression. The current study concludes that, under the conditions tested, high concentrations (2000 and 5000 μg ml(-1)) of CNF cause decreased cell viability and affect the expression of stress- and apoptosis-associated molecular markers.
653 $aAnimals Apoptosis
653 $aApoptosis/genetics
653 $aCattle Cellulose/pharmacology
653 $aDrug effects
653 $aFibroblasts/cytology
653 $aFibroblasts/drug
700 1 $aRAPOSO, N. R. B.
700 1 $aBRAYNER, R.
700 1 $aTEIXEIRA, E. M.
700 1 $aOLIVEIRA, V.
700 1 $aQUINTAO, C. C. R.
700 1 $aCAMARGO, L. S. de A.
700 1 $aMATTOSO, L. H. C.
700 1 $aBRANDAO, H. de M.
773 $tNanotechnology$gv. 24, n. 7, 075103, 2013.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
08/02/2009 |
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Data da última atualização: |
16/10/2018 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MENEZES, A. A.; ACCIOLY, L. J. O.; SILVA, A. B. da; LOPES, H. L.; SILVA, J. A. |
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Afiliação: |
A. A Menezes, estagiário Embrapa Solos; Luciano José de Oliveira Accioly, Embrapa Solos UEP Recife; Ademar Barros da Silva, Embrapa Solos UEP Recife; H. L. Lopes, UNIVASF; Júlia Alves Silva, Estagiária Embrapa Solos. |
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Título: |
Uso e cobertura das terras do município de Olho d´Água das Flores, Alagoas. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In:SIMPÓSIO REGINAL DE GEOPROCESSAMENTO E SENSORIAMENTO REMOTO, 4., 12-5 AGO. 2008, Aracaju, SE. Geonordeste: geotecnologias e meio ambiente. Aracaju, SE: Embrapa Tabuleiros Costeiros, 2008. |
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Idioma: |
Português |
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Palavras-Chave: |
AL; Cobertura da terra; Olho d´Água; Uso de solo. |
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Thesagro: |
Sensoriamento Remoto; Vegetação Nativa. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/184457/1/USO-OLHO-DAGUA-ok.pdf
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Marc: |
LEADER 00763nam a2200217 a 4500
001 1328369
005 2018-10-16
008 2008 bl uuuu u00u1 u #d
100 1 $aMENEZES, A. A.
245 $aUso e cobertura das terras do município de Olho d´Água das Flores, Alagoas.
260 $aIn:SIMPÓSIO REGINAL DE GEOPROCESSAMENTO E SENSORIAMENTO REMOTO, 4., 12-5 AGO. 2008, Aracaju, SE. Geonordeste: geotecnologias e meio ambiente. Aracaju, SE: Embrapa Tabuleiros Costeiros$c2008
650 $aSensoriamento Remoto
650 $aVegetação Nativa
653 $aAL
653 $aCobertura da terra
653 $aOlho d´Água
653 $aUso de solo
700 1 $aACCIOLY, L. J. O.
700 1 $aSILVA, A. B. da
700 1 $aLOPES, H. L.
700 1 $aSILVA, J. A.
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