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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
17/07/2013 |
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Data da última atualização: |
08/09/2021 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BRACKETT, B. G.; KESKINTEPE, L.; SIMPLÍCIO, A. A.; LUVONI, G. G. |
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Afiliação: |
Department of Physiology & Pharmacology, College of Veterinary Medicine, UGA, Athens, GA 30602, USA; Department of Physiology & Pharmacology, College of Veterinary Medicine, UGA, Athens, GA 30602, USA; AURINO ALVES SIMPLÍCIO, CNPC; Instituto di Clinical Ostetrica e Ginecologica Veterinaria, Universita? degli Studi di Milano, Milano, Italy. |
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Título: |
Influences of culture components on the development of bovine blastocysts in defined conditions. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Theriogenology, v. 47, n. 1, p. 274, Jan., 1997. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Experiments were conducted to determine influences on in vitro production (IVP) of bovine embryos of glucose (glc), citrate (c), glutathione (GSH), and growth factors, EGF and PDGF, during 9 d culture in defined conditions. Oocytes, aspirated from follicles (2-5 mm) at slaughter, with homogeneous cytoplasm and 2-3 layers of cumulus cells were washed twice in Tyrodes with 400 mug PVA/ml and 112 mug sodium pyruvate/ml and incubated in 100 mul of mTCM 199 + 10 mug oFSH and 5 mug bLH (NHPP, NIDDK, NICHD, USDA)/ml for 24 h before insemination. Then, after 6 h co-culture with heparin-capacitated sperm, ova were cultured in 50 mul drops of either c-SOF+NEA (control) (Biol.Reprod. 52:1410-1417) or c-SOF+NEA without glc and c (I), or without glc (II) for 5 d; then, developing embryos were transferred into c-SOF+NEA +/- GSH for 4 d. In another trial EGF or PDGF were added to c-SOF+NEA for the last 4 d. Culture media were renewed (50% by volume) every 24 h. Proportions of oocytes that cleaved (C) by 48 h, reached morulae (M) by 120 h, blastocysts (B) by 168 h, and expanded B (EB) by 216 h, and, of M that proceeded to B and EB were analyzed by ''Sigma Stat'' by means of ANOVA; differences among the treatments were analyzed by Bonferroni-t test. Removal of either glc and c, or glc, compromised results, e.g. C of 72.3 % for I, 64.8 % for II, and 86.1 % for control. Addition of GSH 5 d after beginning embryo culture was not beneficial for B development after initial culture in I, 31.3%, or II, 25.2%. Significantly more B and EB were obtained after 9 d in c-SOF+NEA (40.8 % and 34.1 %, P<0.05). Evaluation of development rates of d 5 M to B stages on d 7, 8, 9 revealed more M cultured in I followed by no GSH reached growing B stage by d 7 (27.3 %) than for other experimental groups (11.6 % for I with GSH, 14.9 % for II without GSH, and 18.9 % for II with GSH), but development was faster in c-SOF+NEA (39.6 %) (P<0.05). Early inclusion of citrate was found to enhance M to B development from d 5 to d 8 and, d 9 regardless of other treatments after d 5. In another experiment, different concentrations (0.05, 0.5, 5.0 ng/ml) of EGF or PDGF were added to c-SOF+NEA for the last 4 d of culture. The lowest concentration of either EGF or PDGF resulted in lower (P<0.05) blastocyst development (33.3 % for EGF and 46.6 % for PDGF) compared to the highest concentration (60.0 % and 80.0 %, respectively). EB development was significantly higher (P<0.05) in media containing either growth factor at 5 ng/ml; 32 of 60 M (53.3 %) reached EB with EGF, and 28 (46.6 %) of 60 M reached EB with PDGF present during the final 4 d. Results demonstrated positive influences of glc and c, absence of beneficial effect of GSH, variable patterns in M to B progression, and efficacy of EGF and PDGF in enhancing EB development. MenosAbstract: Experiments were conducted to determine influences on in vitro production (IVP) of bovine embryos of glucose (glc), citrate (c), glutathione (GSH), and growth factors, EGF and PDGF, during 9 d culture in defined conditions. Oocytes, aspirated from follicles (2-5 mm) at slaughter, with homogeneous cytoplasm and 2-3 layers of cumulus cells were washed twice in Tyrodes with 400 mug PVA/ml and 112 mug sodium pyruvate/ml and incubated in 100 mul of mTCM 199 + 10 mug oFSH and 5 mug bLH (NHPP, NIDDK, NICHD, USDA)/ml for 24 h before insemination. Then, after 6 h co-culture with heparin-capacitated sperm, ova were cultured in 50 mul drops of either c-SOF+NEA (control) (Biol.Reprod. 52:1410-1417) or c-SOF+NEA without glc and c (I), or without glc (II) for 5 d; then, developing embryos were transferred into c-SOF+NEA +/- GSH for 4 d. In another trial EGF or PDGF were added to c-SOF+NEA for the last 4 d. Culture media were renewed (50% by volume) every 24 h. Proportions of oocytes that cleaved (C) by 48 h, reached morulae (M) by 120 h, blastocysts (B) by 168 h, and expanded B (EB) by 216 h, and, of M that proceeded to B and EB were analyzed by ''Sigma Stat'' by means of ANOVA; differences among the treatments were analyzed by Bonferroni-t test. Removal of either glc and c, or glc, compromised results, e.g. C of 72.3 % for I, 64.8 % for II, and 86.1 % for control. Addition of GSH 5 d after beginning embryo culture was not beneficial for B development after initial culture in I,... Mostrar Tudo |
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Palavras-Chave: |
Tecnologia de embrião. |
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Thesagro: |
Bovino; Embrião animal. |
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Thesaurus Nal: |
Cattle; Embryo culture; embryo transfer. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03493nam a2200217 a 4500
001 1962286
005 2021-09-08
008 1997 bl uuuu u00u1 u #d
100 1 $aBRACKETT, B. G.
245 $aInfluences of culture components on the development of bovine blastocysts in defined conditions.$h[electronic resource]
260 $aTheriogenology, v. 47, n. 1, p. 274, Jan., 1997.$c1997
520 $aAbstract: Experiments were conducted to determine influences on in vitro production (IVP) of bovine embryos of glucose (glc), citrate (c), glutathione (GSH), and growth factors, EGF and PDGF, during 9 d culture in defined conditions. Oocytes, aspirated from follicles (2-5 mm) at slaughter, with homogeneous cytoplasm and 2-3 layers of cumulus cells were washed twice in Tyrodes with 400 mug PVA/ml and 112 mug sodium pyruvate/ml and incubated in 100 mul of mTCM 199 + 10 mug oFSH and 5 mug bLH (NHPP, NIDDK, NICHD, USDA)/ml for 24 h before insemination. Then, after 6 h co-culture with heparin-capacitated sperm, ova were cultured in 50 mul drops of either c-SOF+NEA (control) (Biol.Reprod. 52:1410-1417) or c-SOF+NEA without glc and c (I), or without glc (II) for 5 d; then, developing embryos were transferred into c-SOF+NEA +/- GSH for 4 d. In another trial EGF or PDGF were added to c-SOF+NEA for the last 4 d. Culture media were renewed (50% by volume) every 24 h. Proportions of oocytes that cleaved (C) by 48 h, reached morulae (M) by 120 h, blastocysts (B) by 168 h, and expanded B (EB) by 216 h, and, of M that proceeded to B and EB were analyzed by ''Sigma Stat'' by means of ANOVA; differences among the treatments were analyzed by Bonferroni-t test. Removal of either glc and c, or glc, compromised results, e.g. C of 72.3 % for I, 64.8 % for II, and 86.1 % for control. Addition of GSH 5 d after beginning embryo culture was not beneficial for B development after initial culture in I, 31.3%, or II, 25.2%. Significantly more B and EB were obtained after 9 d in c-SOF+NEA (40.8 % and 34.1 %, P<0.05). Evaluation of development rates of d 5 M to B stages on d 7, 8, 9 revealed more M cultured in I followed by no GSH reached growing B stage by d 7 (27.3 %) than for other experimental groups (11.6 % for I with GSH, 14.9 % for II without GSH, and 18.9 % for II with GSH), but development was faster in c-SOF+NEA (39.6 %) (P<0.05). Early inclusion of citrate was found to enhance M to B development from d 5 to d 8 and, d 9 regardless of other treatments after d 5. In another experiment, different concentrations (0.05, 0.5, 5.0 ng/ml) of EGF or PDGF were added to c-SOF+NEA for the last 4 d of culture. The lowest concentration of either EGF or PDGF resulted in lower (P<0.05) blastocyst development (33.3 % for EGF and 46.6 % for PDGF) compared to the highest concentration (60.0 % and 80.0 %, respectively). EB development was significantly higher (P<0.05) in media containing either growth factor at 5 ng/ml; 32 of 60 M (53.3 %) reached EB with EGF, and 28 (46.6 %) of 60 M reached EB with PDGF present during the final 4 d. Results demonstrated positive influences of glc and c, absence of beneficial effect of GSH, variable patterns in M to B progression, and efficacy of EGF and PDGF in enhancing EB development.
650 $aCattle
650 $aEmbryo culture
650 $aembryo transfer
650 $aBovino
650 $aEmbrião animal
653 $aTecnologia de embrião
700 1 $aKESKINTEPE, L.
700 1 $aSIMPLÍCIO, A. A.
700 1 $aLUVONI, G. G.
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| 1. |  | KUHN, J.; ADKINS, S.; AGWANDA, B. R.; KUBRUSLI, R. A.; ALKHOVSKY, S. V.; AMARASINGHE, G. K.; TATJANA, A.-Z.; AYLLÓN, M. A.; BAHL, J.; BALKEMA BUSCHMANN, A.; BALLINGER, M. J.; BASLER, C. F.; BAVARI, S.; BEER, M.; BEJERMAN, N.; BENNETT, A. J.; DBENTE, D. A.; BERGERON, É.; BIRD, B. H.; BLAIR, C. D.; BLASDELL, K. R.; BLYSTAD, D. R.; BOJKO, J.; BORTH, W. B.; BRADFUTE, S.; BREYTA, R.; BRIESE, T.; BROWN, P. A.; BROWN, J. K.; BUCHHOLZ, U. J.; BUCHMEIER, M. J.; ABUKREYEV, A.; BURT, F.; BÜTTNER, C.; CALISHER, C. H.; CAO, M.; CASAS, I.; CHANDRAN, K.; CHARREL, R. N.; CHENG, Q.; CHIAKI, Y.; CHIAPELLO, M.; CHOI, I. R.; CIUFO, M.; CLEGG, J. C. S.; CROZIER, I.; BÓ, E. D.; DE LA TORRE, J. C.; LAMBALLERIE, X. de; SWART, R. L. de; DEBAT, H.; DHEILLY, N. M.; DI CICCO, E.; DI PAOLA, N.; DI SERIO, F.; DIETZGEN, R. G.; DIGIARO, M.; DOLNIK, O.; DREBOT, M. A.; DREXLER, J. F.; DUNDON, W. G.; DUPREX, W. P.; DÜRRWALD, R.; DYE, J. M.; EASTON, A. J.; EBIHARA, H.; ELBEAINO, T.; ERGÜNAY, K.; FERGUSON, H. W.; FOOKS, A. R.; FORGIA, M.; FORMENTY, P. B. H.; FRÁNOVÁ, J.; ASTUA, J. de F.; FU, J.; FÜRL, S.; ZACHERT, S. G.; GĀO, G. F.; GARCÍA, M. L.; SASTRE, A. G.; GARRISON, A. R.; GASKIN, T.; GONZALE, J. P. J.; GRIFTHS, A.; GOLDBERG, T. L.; GROSCHUP, M. H.; GÜNTHER, S.; HALL, R. A.; HAMMOND, J.; HAN, O.; HEPOJOKI, J.; HEWSON, R.; HONG, J.; HONG, N.; HORIE, M.; HU, J. S.; HU, T.; HUGHES, H. R.; HÜTTNER, F.; HYNDMAN, T. H.; ILYAS, M.; JALKANEN, R.; JIĀNG, D.; JONSON, G. J.; JUNGLEN, S.; KADONO, F.; KAUKINEN, K. H.; KAWATE, M.; KLEMPA, B.; KLINGSTRÖM, J.; KOBINGER, G.; KOLONIUK, I.; KONDŌ, H.; KOONIN, V.; KRUPOVIC, M.; KUBOTA, K.; KURATH, G.; LAENEN, L.; LAMBERT, A. J.; LANGEVIN, S. L.; LEE, B.; LEFKOWITZ, E. J.; LI, L.; LEROY, E. M.; LI, S.; LÍ, J.; LIU, H.; LUKASHEVICH, I. S.; MAES, P.; SOUZA, W. M. de; MARKLEWITZ, M.; MARSHALL, S. H.; MARZANO, S. L.; MASSART, S.; MCCAULEY, J. W.; MELZER, M.; MILLER, K. M.; MING, T. J.; MIRAZIMI, A.; MORDECAI, G. J.; MÜHLBACH, H. P.; MÜHLBERGER, E.; NAIDU, R.; NATSUAKI, T.; NAVARRO, J. A.; NETESOV, S. Y V.; NEUMANN, G.; NOWOTNY, N.; NUNES, M. R. T.; VELARDE, A. O.; PALACIOS, G.; PALLÁS, V.; PÁLYI, B.; PAPA, A.; PARASKEVOPOULOU, S.; PARK, A. C.; PARRISH, C. R.; PATTERSON, D. A.; CORRÊA, A. P.; PAWESKA, J. T.; PAYNE, S.; PERACCHIO, C.; PÉREZ, D. R.; POSTLER, T. S.; QI, L.; RADOSHITZKY, S. R.; RESENDE, R. O.; REYES, C. A.; RIMA, B. K.; LUNA, G. R.; ROMANOWSKI, V.; ROTA, P.; RUBBENSTROTH, D.; RUBINO, L.; RUNSTADLER, J. A.; SABANADZOVIC, S.; SALL, A. A.; SALVATO, M. S.; SANG, R.; SASAYA, T.; SCHULZE, A. D.; SCHWEMMLE, M.; SHI, M.; SHÍ, X.; SHIMOMOTO, Y.; SHIRAKO, Y.; SIDDELL, S. G.; SIMMONDS, P.; SIRONI, M.; SMAGGHE, G.; SMITHER, S.; SONG, J. W.; SPANN, K.; SPENGLER, J. R.; STENGLEIN, M. D.; STONE, D. M.; SUGANO, J.; TABATA, A.; TAKADA, A.; TAKEUCHI, S.; TCHOUASSI, D. P.; TEFER, A.; TESH, R. B.; THORNBURG, N. J.; TOMITAKA, Y.; TOMONAGA, K.; TORDO, N.; TORTO, B.; TOWNER, J. S.; TSUDA, S.; TU, C.; TURINA, M.; TZANETAKIS, I. E.; UCHIDA, J.; USUGI, T.; VAIRA, A. M.; VALLINO, M.; VAN DEN HOOGEN, B.; VARSANI, A.; VASILAKIS, N.; VERBEEK, M.; BARGEN, S. V.; WADA, J.; WAHL, V.; WALKER, P. J.; WANG, L. F.; WANG, G.; WANG, Y.; WANG, Y.; WAQAS, M.; WÈI, T.; WEN, S.; WHITFELD, A. E.; WILLIAMS, J. V.; WOLF, Y. I.; WU, J.; XU, L.; YANAGISAWA, H.; YANG, C.; YANG, Z.; ZERBINI, F. M.; ZHAI, L.; ZHANG, Y. Z.; ZHANG, O.; ZHANG, J.; ZHANG, Z.; ZHOU, X. 2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirale. Archives of Virology, August, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
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