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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
08/07/2013 |
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Data da última atualização: |
19/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MAFRA, V.; MARTINS, P. K; FRANCISCO, C. S.; RIBEIRO-ALVES, M.; ASTUA, J. de F.; MACHADO, M. A. |
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Afiliação: |
VALÉRIA MAFRA, IAC; POLYANA K MARTINS, IAC; CAROLINA S FRANCISCO, IAC; MARCELO RIBEIRO-ALVES, FIOCRUZ; JULIANA DE FREITAS ASTUA, CNPMF; MARCOS A MACHADO, IAC. |
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Título: |
Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
BMC Genomics, p.14-247, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus huanglongbing (HLB) disease is caused by endogenous, phloem-restricted, Gram negative, uncultured bacteria named Candidatus Liberibacter africanus (CaLaf), Ca. L. asiaticus (CaLas), and Ca. L. americanus (CaLam), depending on the continent where the bacteria were first detected. The Asian citrus psyllid vector, Diaphorina citri, transmits CaLas and CaLam and both Liberibacter species are present in Brazil. Several studies of the transcriptional response of citrus plants manifesting HLB symptoms have been reported, but only for CaLas infection. This study evaluated the transcriptional reprogramming of a susceptible genotype of sweet orange challenged with CaLam, using a customized 385K microarray containing approximately 32,000 unigene transcripts. We analyzed global changes in gene expression of CaLam-infected leaves of sweet orange during the symptomatic stage of infection and compared the results with previously published microarray studies that used CaLas-infected plants. Twenty candidate genes were selected to validate the expression profiles in symptomatic and asymptomatic PCR-positive leaves infected with CaLas or CaLam. |
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Palavras-Chave: |
HLB. |
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Thesagro: |
Doença de planta; Fruta citrica. |
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Thesaurus Nal: |
Candidatus Liberibacter africanus; Citrus. |
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Categoria do assunto: |
-- |
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Marc: |
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001 1961588
005 2023-05-19
008 2013 bl uuuu u00u1 u #d
100 1 $aMAFRA, V.
245 $aCandidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype.$h[electronic resource]
260 $c2013
520 $aCitrus huanglongbing (HLB) disease is caused by endogenous, phloem-restricted, Gram negative, uncultured bacteria named Candidatus Liberibacter africanus (CaLaf), Ca. L. asiaticus (CaLas), and Ca. L. americanus (CaLam), depending on the continent where the bacteria were first detected. The Asian citrus psyllid vector, Diaphorina citri, transmits CaLas and CaLam and both Liberibacter species are present in Brazil. Several studies of the transcriptional response of citrus plants manifesting HLB symptoms have been reported, but only for CaLas infection. This study evaluated the transcriptional reprogramming of a susceptible genotype of sweet orange challenged with CaLam, using a customized 385K microarray containing approximately 32,000 unigene transcripts. We analyzed global changes in gene expression of CaLam-infected leaves of sweet orange during the symptomatic stage of infection and compared the results with previously published microarray studies that used CaLas-infected plants. Twenty candidate genes were selected to validate the expression profiles in symptomatic and asymptomatic PCR-positive leaves infected with CaLas or CaLam.
650 $aCandidatus Liberibacter africanus
650 $aCitrus
650 $aDoença de planta
650 $aFruta citrica
653 $aHLB
700 1 $aMARTINS, P. K
700 1 $aFRANCISCO, C. S.
700 1 $aRIBEIRO-ALVES, M.
700 1 $aASTUA, J. de F.
700 1 $aMACHADO, M. A.
773 $tBMC Genomics, p.14-247, 2013.
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| 2. |  | XAVIER, N. G.; SIMEONE, M. L. F.; QUEIROZ, V. A. V.; SILVA, C. B. da; MAGALHAES, J. V. de; PASTINA, M. M. Using multispectral images to assess bioactive compounds in sorghum. In: INTERNATIONAL WORKSHOP ON HYPERSPECTRAL IMAGING, 1., 2021, Campinas. Resumos. Campinas: Universidade Estadual de Campinas, 2021.| Tipo: Resumo em Anais de Congresso |
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