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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
24/06/2013 |
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Data da última atualização: |
07/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DELABONA, P. da S.; PIROTA, R. D. P. B.; CODIMA, C. A.; TREMACOLDI, C. R.; RODRIGUES, A.; FARINAS, C. S. |
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Afiliação: |
PRISCILA DA SILVA DELABONA, CNPDIA/UFSCAR; ROSANGELA DONIZETE PERPETUA BUZON PIROTA, CNPDIA/UFSCAR; CARLA ALOIA CODIMA, CNPDIA/UFSCAR; CELIA REGINA TREMACOLDI, CPATU; ANDRÉ RODRIGUES, UNIVERSIDADE ESTADUAL DE SANTA CRUZ; CRISTIANE SANCHEZ FARINAS, CNPDIA. |
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Título: |
Effect of initial moisture content on two Amazon rainforest Aspergillus strains cultivated on agro-industrial residues: Biomass-degrading enzymes production and characterization. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Industrial Crops and Products, v. 42, p. 236-242, 2013. |
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DOI: |
http://dx.doi.org/10.1016/j.indcrop.2012.05.035 |
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Idioma: |
Inglês |
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Conteúdo: |
Production of biomass-degrading enzymes using inexpensive and readily available agricultural residues as substrates for solid-state fermentation (SSF) can contribute to a broader application of enzymes for the conversion of biomass into biofuels and chemicals. Among the operational parameters that affect SSF process efficiency, moisture content is one of the most important. This work evaluates the effect of initial moisture content on two Aspergillus strains (Aspergillus niger P47C3 and Aspergillus fumigatus P40M2), isolated from the Amazon rainforest and grown under SSF. Analyses were made of the biomass-degrading enzymes produced using different agro-industrial residues as carbon sources (wheat bran, sugar cane bagasse, soybean bran, and orange bagasse). The enzymatic complex produced by a selected strain of A. fumigatus was characterized in terms of optimum pH and temperature, and thermal stability. The most effective carbon sources for multienzyme production during Aspergillus cultivation were wheat and soybean bran, as well as a 1:1 mixture of sugar cane bagasse and wheat bran. Much higher activity values were achieved for β-glucosidase (105.8 IU/g) and xylanase (1055.6 IU/g) when wheat bran with 50% initial moisture content was used as substrate. Under this condition, endoglucanase and total cellulase activity values were 56.6 IU/g and 5.0 FPU/g, respectively. Characterization of the crude enzymatic complex showed that the A. fumigatus P40M2 enzymes were active in the acidic pH range, with maximal activities at the range of 50?65 °C, demonstrating the potential of the organism for the production of acidophilic and thermophilic biomass-degrading enzymes. MenosProduction of biomass-degrading enzymes using inexpensive and readily available agricultural residues as substrates for solid-state fermentation (SSF) can contribute to a broader application of enzymes for the conversion of biomass into biofuels and chemicals. Among the operational parameters that affect SSF process efficiency, moisture content is one of the most important. This work evaluates the effect of initial moisture content on two Aspergillus strains (Aspergillus niger P47C3 and Aspergillus fumigatus P40M2), isolated from the Amazon rainforest and grown under SSF. Analyses were made of the biomass-degrading enzymes produced using different agro-industrial residues as carbon sources (wheat bran, sugar cane bagasse, soybean bran, and orange bagasse). The enzymatic complex produced by a selected strain of A. fumigatus was characterized in terms of optimum pH and temperature, and thermal stability. The most effective carbon sources for multienzyme production during Aspergillus cultivation were wheat and soybean bran, as well as a 1:1 mixture of sugar cane bagasse and wheat bran. Much higher activity values were achieved for β-glucosidase (105.8 IU/g) and xylanase (1055.6 IU/g) when wheat bran with 50% initial moisture content was used as substrate. Under this condition, endoglucanase and total cellulase activity values were 56.6 IU/g and 5.0 FPU/g, respectively. Characterization of the crude enzymatic complex showed that the A. fumigatus P40M2 enzymes were active in... Mostrar Tudo |
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Palavras-Chave: |
Resíduo agroindustrial. |
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Thesagro: |
Biomassa. |
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Thesaurus Nal: |
Amazonia. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 02498naa a2200229 a 4500
001 1960431
005 2022-11-07
008 2013 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1016/j.indcrop.2012.05.035$2DOI
100 1 $aDELABONA, P. da S.
245 $aEffect of initial moisture content on two Amazon rainforest Aspergillus strains cultivated on agro-industrial residues$bBiomass-degrading enzymes production and characterization.$h[electronic resource]
260 $c2013
520 $aProduction of biomass-degrading enzymes using inexpensive and readily available agricultural residues as substrates for solid-state fermentation (SSF) can contribute to a broader application of enzymes for the conversion of biomass into biofuels and chemicals. Among the operational parameters that affect SSF process efficiency, moisture content is one of the most important. This work evaluates the effect of initial moisture content on two Aspergillus strains (Aspergillus niger P47C3 and Aspergillus fumigatus P40M2), isolated from the Amazon rainforest and grown under SSF. Analyses were made of the biomass-degrading enzymes produced using different agro-industrial residues as carbon sources (wheat bran, sugar cane bagasse, soybean bran, and orange bagasse). The enzymatic complex produced by a selected strain of A. fumigatus was characterized in terms of optimum pH and temperature, and thermal stability. The most effective carbon sources for multienzyme production during Aspergillus cultivation were wheat and soybean bran, as well as a 1:1 mixture of sugar cane bagasse and wheat bran. Much higher activity values were achieved for β-glucosidase (105.8 IU/g) and xylanase (1055.6 IU/g) when wheat bran with 50% initial moisture content was used as substrate. Under this condition, endoglucanase and total cellulase activity values were 56.6 IU/g and 5.0 FPU/g, respectively. Characterization of the crude enzymatic complex showed that the A. fumigatus P40M2 enzymes were active in the acidic pH range, with maximal activities at the range of 50?65 °C, demonstrating the potential of the organism for the production of acidophilic and thermophilic biomass-degrading enzymes.
650 $aAmazonia
650 $aBiomassa
653 $aResíduo agroindustrial
700 1 $aPIROTA, R. D. P. B.
700 1 $aCODIMA, C. A.
700 1 $aTREMACOLDI, C. R.
700 1 $aRODRIGUES, A.
700 1 $aFARINAS, C. S.
773 $tIndustrial Crops and Products$gv. 42, p. 236-242, 2013.
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| 5. |  | SILVA, M. C.; FERREIRA, M. D.; TESTEZLAF, R.; MAGALHÃES, A. M. de; FRANCO, A. T. O. Optimizing cleaning efficiency at a fresh market tomato packing line. In: INTERNATIONAL CONFERENCE OF AGRICULTURAL ENGINEERING; BRAZILIAN CONGRESS OF AGRICULTURAL ENGINEERING, 37.; INTERNATIONAL LIVESTOCK ENVIRONMENT SYMPOSIUM - ILES, 8., 2008, Foz do Iguaçu. Technology for all: sharing the knowledge for development. Proceedings... Foz do Iguaçu: SBEA, 2008. não paginado. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
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| 6. | | SILVA, M. C. da; FRANCO, A. T. O.; FERREIRA, M. D.; MAGALHÃES, A. M. de; TESTEZLAF, R. Otimização da eficiência de limpeza em equipamento de beneficiamento de tomate de mesa. Engenharia Agrícola, Jaboticabal, v. 28, n. 4, p. 750-758, 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
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| 7. | | FRANCO, D. F.; MAGALHÃES, A. M. de; TERRES, A. L.; VERNETTI JÚNIOR, F. J.; RANGEL, P. H.; PINHEIRO, B.; CUTRIM, V.; MOURA NETO, F. P. Ensaio estadual de valor de cultivo e uso de linhagens promissoras de arroz irrigado da Embrapa-Clima Temperado, Rio Grande do Sul, safra 2001/2001. In: CONGRESSO BRASILEIRO DE ARROZ IRRIGADO, 2.; REUNIÃO DA CULTURA DO ARROZ IRRIGADO, 24., 2001, Porto Alegre. Anais... Porto Alegre: IRGA, 2001. p. 69-71.| Biblioteca(s): Embrapa Arroz e Feijão. |
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