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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
07/05/2013 |
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Data da última atualização: |
17/01/2024 |
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Autoria: |
SILVA, G. M.; ARAÚJO, V. R.; DUARTE, A. B. G.; CHAVES, R. N.; SILVA, C. M. G.; LOBO, C. H.; ALMEIDA, A. P.; MATOS, M. H. T.; TAVARES, L. M. T.; CAMPELLO, C. C.; FIGUEIREDO, J. R. |
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Título: |
Ascorbic acid improves the survival and in vitro growth of isolated caprine preantral follicles. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Animal Reproduction, Belo Horizonte, v. 8, n. 1/2, p. 14-24, Jan./Jun. 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The present study aims to investigate the influence of two concentrations of ascorbic acid on the survival, growth, antral formation and mRNA expression of the matrix metalloproteinases-9 (MMP-9) and their tissue inhibitor-2 (TIMP-2) on caprine preantral follicles during long-term in vitro culture. Isolated preantral follicles were individually cultured without or with ascorbic acid at 50 µg/ml (AA50) or 100 µg/ml (AA100) during 18 days. The parameters evaluated were follicular viability, growth, antrum formation and extruded oocytes. The genes MMP-9 and TIMP-2 were quantified by real-time polymerase chain reaction (qPCR) after 18 days of culture in the control medium (MEM+) or ascorbic acid (50 or 100 µg/ml) and in fresh control (non cultured). At the end of culture, AA50 significantly increased the percentage of viable follicles compared with other treatments. Moreover, mean daily increase in follicular diameter (µm/day) was significantly higher in the presence of both concentrations of ascorbic acid than in MEM+alone. Higher rates of antral formation and lower percentages of extruded oocytes were observed in medium containing AA50 compared with control medium. Real Time RT-PCR assays showed that AA50 increases MMP-9 expression significantly compared with fresh control and MEM+ alone. In conclusion, ascorbic acid at 50 µg/ml was very important for the maintenance of caprine preantral follicle viability and development after in vitro culture and influences in vitro the enzymes involved with basement membrane remodeling. MenosAbstract: The present study aims to investigate the influence of two concentrations of ascorbic acid on the survival, growth, antral formation and mRNA expression of the matrix metalloproteinases-9 (MMP-9) and their tissue inhibitor-2 (TIMP-2) on caprine preantral follicles during long-term in vitro culture. Isolated preantral follicles were individually cultured without or with ascorbic acid at 50 µg/ml (AA50) or 100 µg/ml (AA100) during 18 days. The parameters evaluated were follicular viability, growth, antrum formation and extruded oocytes. The genes MMP-9 and TIMP-2 were quantified by real-time polymerase chain reaction (qPCR) after 18 days of culture in the control medium (MEM+) or ascorbic acid (50 or 100 µg/ml) and in fresh control (non cultured). At the end of culture, AA50 significantly increased the percentage of viable follicles compared with other treatments. Moreover, mean daily increase in follicular diameter (µm/day) was significantly higher in the presence of both concentrations of ascorbic acid than in MEM+alone. Higher rates of antral formation and lower percentages of extruded oocytes were observed in medium containing AA50 compared with control medium. Real Time RT-PCR assays showed that AA50 increases MMP-9 expression significantly compared with fresh control and MEM+ alone. In conclusion, ascorbic acid at 50 µg/ml was very important for the maintenance of caprine preantral follicle viability and development after in vitro culture and influences in vitr... Mostrar Tudo |
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Palavras-Chave: |
Ácido ascorbico; Folículo pré-antral; MMP-9. |
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Thesagro: |
Caprino; Cultura in vitro; Reprodução animal; Vitamina C. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02509naa a2200325 a 4500
001 1957249
005 2024-01-17
008 2011 bl uuuu u00u1 u #d
100 1 $aSILVA, G. M.
245 $aAscorbic acid improves the survival and in vitro growth of isolated caprine preantral follicles.$h[electronic resource]
260 $c2011
520 $aAbstract: The present study aims to investigate the influence of two concentrations of ascorbic acid on the survival, growth, antral formation and mRNA expression of the matrix metalloproteinases-9 (MMP-9) and their tissue inhibitor-2 (TIMP-2) on caprine preantral follicles during long-term in vitro culture. Isolated preantral follicles were individually cultured without or with ascorbic acid at 50 µg/ml (AA50) or 100 µg/ml (AA100) during 18 days. The parameters evaluated were follicular viability, growth, antrum formation and extruded oocytes. The genes MMP-9 and TIMP-2 were quantified by real-time polymerase chain reaction (qPCR) after 18 days of culture in the control medium (MEM+) or ascorbic acid (50 or 100 µg/ml) and in fresh control (non cultured). At the end of culture, AA50 significantly increased the percentage of viable follicles compared with other treatments. Moreover, mean daily increase in follicular diameter (µm/day) was significantly higher in the presence of both concentrations of ascorbic acid than in MEM+alone. Higher rates of antral formation and lower percentages of extruded oocytes were observed in medium containing AA50 compared with control medium. Real Time RT-PCR assays showed that AA50 increases MMP-9 expression significantly compared with fresh control and MEM+ alone. In conclusion, ascorbic acid at 50 µg/ml was very important for the maintenance of caprine preantral follicle viability and development after in vitro culture and influences in vitro the enzymes involved with basement membrane remodeling.
650 $aCaprino
650 $aCultura in vitro
650 $aReprodução animal
650 $aVitamina C
653 $aÁcido ascorbico
653 $aFolículo pré-antral
653 $aMMP-9
700 1 $aARAÚJO, V. R.
700 1 $aDUARTE, A. B. G.
700 1 $aCHAVES, R. N.
700 1 $aSILVA, C. M. G.
700 1 $aLOBO, C. H.
700 1 $aALMEIDA, A. P.
700 1 $aMATOS, M. H. T.
700 1 $aTAVARES, L. M. T.
700 1 $aCAMPELLO, C. C.
700 1 $aFIGUEIREDO, J. R.
773 $tAnimal Reproduction, Belo Horizonte$gv. 8, n. 1/2, p. 14-24, Jan./Jun. 2011.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 8 | |
| 1. |  | CARDOSO, F. F.; STEIBEL, J. P.; ROSA, G. J. M.; ERNST, C. W.; BATES, R. O.; TEMPELMAN, R. J. Assessment of different selective phenotyping design strategies for genomics studies with outbred F2 populations. Journal of Animal Science, Savoy, v. 85, p. 373, 2007. Supplement 1.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 2. | | CARDOSO, F. F.; STEIBEL, J. P.; ROSA, G. J. de M.; ERNST, C.; BATES, R.; TEMPELMAN, R. J. Estratégias de perfilamento transcricional seletivo e de análise de dados para estudos de genética genômica em populações exogâmicas. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 45., 2008, Lavras, MG. Biotecnologia e sustentabilidade : anais dos resumos. Brasília, DF: Sociedade Brasileira de Zootecnia, 2008. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Pecuária Sul. |
|  |
| 3. | | CARDOSO, F. F.; ROSA, G. J. M.; STEIBEL, J. P.; ERNST, C. W.; BATES, R. O.; TEMPELMAN, R. J. Selective transcriptional profiling and data analysis strategies for expression quantitative trait loci mapping in outbred F2 populations. Genetics, Pittsburgh, v. 180, n. 3, p. 1679-1690, Nov. 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 4. | | ERNST, C. W.; STEIBLE, J. P.; RAMOS, A. M.; TEMPELMAN, R. J.; CARDOSO, F. F.; ROSA, G. J. M.; EDWARDS, D. B.; BATES, R. O. Expression QTL (eQTL) analysis for identifying genes controlling economically important traits in pigs. In: NATIONAL SWINE IMPROVEMENT FEDERATION CONFERENCE AND ANNUAL MEETING, 32., 2007, Kansas City. [Electronic proceedings]... Knoxville: NSIF, 2007. Metas 2008.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 5. | | ERNST, C. W.; STEIBEL, J. P.; ROSA, G. J. M.; TEMPELMAN, R. J.; BATES, R. O.; RILINGTON, V. D.; RAGAVENDRAN, A.; RANEY, N. E.; RAMOS, A. M.; CARDOSO, F. F.; EDWARDS, D. B. Genome-wide expression QTL (eQYL) analysis of loin muscle tissue to identify candidate genes in pigs. In: ADSA CSAS ASAS JOINT ANNUAL MEETING, 2009, Montreal. Electronic abstracts... Champaign: ADSA: ASAS, 2009. LB4. Publicado em: Jornal of Animal Science, Champaign, v. 87, p. ii, 2009. Electronic Supplement 2.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 6. | | STEIBEL, J. P.; BATES, R. O.; ROSA, G. J. M.; TEMPELMAN, R. J.; RILINGTON, V. D.; RAGAVENDRAM, A.; RANEY, N. E.; RAMOS, A. M.; CARDOSO, F. F.; EDWARDS, D. B.; ERNST, C. W. Genome-wide linkage analysis of gene expression of loin muscle tissue identifies candidate genes in pigs. In: WORLD CONGRESS ON GENETICS APPLIED TO LIVESTOCK PRODUCTION, 9., 2010, Leipzig. Proceedings... Leipzig: German Society of Animal Science, 2010. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 7. | | STEIBEL, J. P.; BATES, R. O.; ROSA, G. J. M.; TEMPELMAN, R. J.; RILINGTON, V. D.; RAGAVENDRAN, A.; RANEY, NANCY E.; RAMOS, A. M.; CARDOSO, F. F.; EDWARDS, D. B.; ERNST, C. W. Genome-wide linkage analysis of global gene expression in loin muscle tissue identifies candidate genes in pigs. PLoS ONE, San Francisco, v. 6, n. 2, e16766, Feb. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 8. | | LUNNEY, J.; ROWLAND, B.; TRIBLE, B.; CHOI, I.; ABRAMS, S.; SOUZA, C.; REECY, J.; FRITZ-WATER, E.; KOLTES, J.; EISLEY, C.; TUGGLE, C.; HESS, A.; DUNKELBERGER, J.; DEKKERS, J.; BODDICKER, N.; STEIBEL, J.; ERNST, C.; GUAN, L. L.; BAO, H.; KOMMADATH, A.; STOTHARD, P.; PLASTOW, G.; LADINIG, A.; HARDING, J. C. S. Exploring genetic control of swine responses to viral diseases. In: INTERNATIONAL SOCIETY FOR ANIMAL GENETICS CONFERENCE, 34., 2014, 3., 2012, Xi'an, China. Abstract. Xi'an: ISAG, 2014. p. 5.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pesca e Aquicultura. |
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| Registros recuperados : 8 | |
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| Expressão de busca inválida. Verifique!!! |
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