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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
07/05/2013 |
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Data da última atualização: |
17/01/2024 |
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Autoria: |
LIMA-VERDE, I. B.; ROSSETTO, R.; MATOS, M. H. T.; CELESTINO, J. J. H.; BUENO, J. B.; SILVA, C. M. G.; FAUSTINO, L. R.; MORORÓ, M. B. S.; ARAÚJO, V. T.; CAMPELLO, C. C.; FIGUEIREDO, J. R. |
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Título: |
Androstenedione and follicle stimulating hormone involvement in the viability and development of goat preantral follicles in vitro. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Animal Reproduction, Belo Horizonte, v. 7, n. 2, p. 80-89, Apr./Jun. 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study investigated the effects of androstenedione and follicle-stimulating hormone (FSH) on the viability and growth of caprine preantral follicles. Ovarian tissues were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) containing androstenedione (0, 1, 10, 50, or 100 ng/ml), FSH (50 ng/ml), or a combination of these two hormones. Cultured and noncultured control tissues were processed for histological and fluorescence analysis. In comparison with noncultured control, a significant reduction was noted in the percentage of normal follicles in all treatments after 1 and 7 days of culture (except treatment with 1 ng/ml of androstenedione for 1 day). As the culture period progressed from 1 to 7 days, treatments with 10 ng/ml of androstenedione + FSH or 50 ng/ml of androstenedione alone maintained the percentage of normal follicles. After 1 day, treatments with 10, 50, or 100 ng/ml of androstenedione + FSH, or with 50 ng/ml of androstenedione alone had more developing follicles than fresh control tissue. When comparing the culture periods, treatments with 1, 10 or 100 ng/ml of androstenedione alone, or FSH alone, or FSH with 1 ng/ml of androstenedione, showed an increase in the percentage of developing follicles. After 1 and 7 days, there were no differences in oocyte and follicular diameter among the treated samples and non-cultured control or MEM+ cultured tissue. Fluorescence analysis demonstrated that only fragments cultured in 50 or 100 ng/ml of androstenedione + FSH had viable preantral follicles similar to those observed in MEM+alone. In conclusion, androstenedione at 50 or 100 ng/ml, either associated with FSH or at 50 ng/ml alone, plays an important role in the maintenance of caprine preantral follicle viability and activation after only a short in vitro culture period. In addition, after 7 days MEM+alone was efficient in the maintenance of viability and in follicular activation, showing the importance of basic medium composition. MenosAbstract: This study investigated the effects of androstenedione and follicle-stimulating hormone (FSH) on the viability and growth of caprine preantral follicles. Ovarian tissues were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) containing androstenedione (0, 1, 10, 50, or 100 ng/ml), FSH (50 ng/ml), or a combination of these two hormones. Cultured and noncultured control tissues were processed for histological and fluorescence analysis. In comparison with noncultured control, a significant reduction was noted in the percentage of normal follicles in all treatments after 1 and 7 days of culture (except treatment with 1 ng/ml of androstenedione for 1 day). As the culture period progressed from 1 to 7 days, treatments with 10 ng/ml of androstenedione + FSH or 50 ng/ml of androstenedione alone maintained the percentage of normal follicles. After 1 day, treatments with 10, 50, or 100 ng/ml of androstenedione + FSH, or with 50 ng/ml of androstenedione alone had more developing follicles than fresh control tissue. When comparing the culture periods, treatments with 1, 10 or 100 ng/ml of androstenedione alone, or FSH alone, or FSH with 1 ng/ml of androstenedione, showed an increase in the percentage of developing follicles. After 1 and 7 days, there were no differences in oocyte and follicular diameter among the treated samples and non-cultured control or MEM+ cultured tissue. Fluorescence analysis demonstrated that only fragments cultured in 50 or 100 ng/ml of andr... Mostrar Tudo |
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Palavras-Chave: |
Esteroidogenese; Foliculo pre-antral; FSH. |
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Thesagro: |
Caprino; Cultura in vitro; Endocrinologia; Hormônio; Ovário; Reprodução animal. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03032naa a2200349 a 4500
001 1957239
005 2024-01-17
008 2010 bl uuuu u00u1 u #d
100 1 $aLIMA-VERDE, I. B.
245 $aAndrostenedione and follicle stimulating hormone involvement in the viability and development of goat preantral follicles in vitro.$h[electronic resource]
260 $c2010
520 $aAbstract: This study investigated the effects of androstenedione and follicle-stimulating hormone (FSH) on the viability and growth of caprine preantral follicles. Ovarian tissues were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) containing androstenedione (0, 1, 10, 50, or 100 ng/ml), FSH (50 ng/ml), or a combination of these two hormones. Cultured and noncultured control tissues were processed for histological and fluorescence analysis. In comparison with noncultured control, a significant reduction was noted in the percentage of normal follicles in all treatments after 1 and 7 days of culture (except treatment with 1 ng/ml of androstenedione for 1 day). As the culture period progressed from 1 to 7 days, treatments with 10 ng/ml of androstenedione + FSH or 50 ng/ml of androstenedione alone maintained the percentage of normal follicles. After 1 day, treatments with 10, 50, or 100 ng/ml of androstenedione + FSH, or with 50 ng/ml of androstenedione alone had more developing follicles than fresh control tissue. When comparing the culture periods, treatments with 1, 10 or 100 ng/ml of androstenedione alone, or FSH alone, or FSH with 1 ng/ml of androstenedione, showed an increase in the percentage of developing follicles. After 1 and 7 days, there were no differences in oocyte and follicular diameter among the treated samples and non-cultured control or MEM+ cultured tissue. Fluorescence analysis demonstrated that only fragments cultured in 50 or 100 ng/ml of androstenedione + FSH had viable preantral follicles similar to those observed in MEM+alone. In conclusion, androstenedione at 50 or 100 ng/ml, either associated with FSH or at 50 ng/ml alone, plays an important role in the maintenance of caprine preantral follicle viability and activation after only a short in vitro culture period. In addition, after 7 days MEM+alone was efficient in the maintenance of viability and in follicular activation, showing the importance of basic medium composition.
650 $aCaprino
650 $aCultura in vitro
650 $aEndocrinologia
650 $aHormônio
650 $aOvário
650 $aReprodução animal
653 $aEsteroidogenese
653 $aFoliculo pre-antral
653 $aFSH
700 1 $aROSSETTO, R.
700 1 $aMATOS, M. H. T.
700 1 $aCELESTINO, J. J. H.
700 1 $aBUENO, J. B.
700 1 $aSILVA, C. M. G.
700 1 $aFAUSTINO, L. R.
700 1 $aMORORÓ, M. B. S.
700 1 $aARAÚJO, V. T.
700 1 $aCAMPELLO, C. C.
700 1 $aFIGUEIREDO, J. R.
773 $tAnimal Reproduction, Belo Horizonte$gv. 7, n. 2, p. 80-89, Apr./Jun. 2010.
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| 1. |  | CORREA, S. B.; CORONADO-FRANCO, K. V.; JÉZÉQUEL, C.; RODRIGUES, A. C.; EVANS, K. O.; GRANGER, J. J.; STEEGE, H. T.; AMARAL, I. L. do; COELHO, L. de S.; WITTMANN, F.; MATOS, F. D. de A.; LIMA FILHO, D. de A.; SALOMÃO, R. P.; CASTILHO, C. V. de; GUEVARA, J. E.; CARIM, M. de J. V.; PHILLIPS, O. L.; PIEDADE, M. T. F.; DEMARCHI, L. O.; SCHÖNGART, J.; REVILLA, J. D. C.; MARTINS, M. P.; IRUME, M. V.; GUIMARÃES, J. R. da S.; RAMOS, J. F.; QUARESMA, A. C.; PITMAN, N. C. A.; LUIZE, B. G.; NOVO, E. M. M. de L.; VENTICINQUE, E. M.; SILVA, T. S. F.; VARGAS, P. N.; MANZATTO, A. G.; REIS, N. F. C.; TERBORGH, J.; CASULA, K. R.; CORONADO, E. N. H.; MONTERO, J. C.; MENDOZA, A. M.; FELDPAUSCH, T. R.; DURGANTE, F. M.; ARBOLEDA, N. C.; MARIMON, B. S.; MARIMON-JUNIOR, B. H.; KILLEEN, T. J.; VASQUEZ, R.; MOSTACEDO, B.; ASSIS, R. L.; AMARAL, D. D. do; HOUSEHOLDER, J. E.; SIMON, M. F.; MEDEIROS, M. B. de; QUEIROZ, H. L. de; LOPES, M. A.; MAGALHÃES, J. L. L.; STEVENSON, P. R.; CINTRA, B. B. L.; ARAUJO-MURAKAMI, A.; BAKER, T. R.; FEITOSA, Y. O.; MOGOLLÓN, H. F.; DUIVENVOORDEN, J. F.; FERREIRA, L. V.; TOLEDO, J. J. de; COMISKEY, J. A.; LOPES, A.; DAMASCO, G.; VICENTINI, A.; VALVERDE, F. C.; GOMES, V. H. F.; ALONSO, A.; DALLMEIER, F.; AGUIAR, D. P. P. de; GRIBEL, R.; LICONA, J. C.; ZEGARRA, B. E. V.; GUEDES, M. C.; CERÓN, C.; THOMAS, R.; MILLIKEN, W.; CAMPELO, W.; ALBUQUERQUE, B. W.; KLITGAARD, B.; TELLO, J. S.; CLAROS, A. F.; RIVAS-TORRES, G.; PHILLIPS, J. F.; HILDEBRAND, P. von; GONZALES, T.; VELA, C. I. A.; HOFFMAN, B.; FLORES, B. M.; POMBO, M. M.; ROCHA, M.; HOLMGREN, M.; CANO, A.; UMAÑA, M. N.; CASAS, L. F.; BALSLEV, H.; GIRALDO, L. E. U.; BIGORNE, R.; OBERDORFF, T.; MALDONADO-OCAMPO, J. A.; ORTEGA, H.; HIDALGO, M.; MARTENS, K.; TORRENTE-VILARA, G.; ZUANON, J.; ACOSTA, A.; AGUDELO, E.; MAURE, S. B.; BASTOS, D. A.; GREGORY, J. B.; CABECEIRA, F. G.; CANTO, A. L. C.; CARVAJAL-VALLEJOS, F. M.; CARVALHO, L. N.; CELLA-RIBEIRO, A.; COVAIN, R.; DIAS, M. S.; DONASCIMIENTO, C.; DÓRIA, C. R. C.; DUARTE, C.; FERREIRA, E. J. G.; GALUCH, A. V.; GIARRIZZO, T.; LEITÃO, R. P.; LUNDBERG, J. G.; MALDONADO, M.; MOJICA, J. I.; MONTAG, L. F. A.; OHARA, W.; PIRES, T. H. S.; POUILLY, M.; PRADA-PEDREROS, S.; QUEIROZ, L. J. de; PY-DANIEL, L. R.; RIBEIRO, F. R. V.; HERRERA, R. R.; ANJOS, M. R. dos; LOURENCO, I. H.; SARMIENTO, J.; SOUSA, L. M.; STEGMANN, L. F.; VALDIVIEZO-RIVERA, J.; VILLA, F.; YUNOKI, T.; TEDESCO, P. A. Floodplain forests drive fruit-eating fish diversity at the Amazon Basin-scale. PNAS, v. 122, n. 3, e2414416122, 2025.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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