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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
18/01/2013 |
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Data da última atualização: |
16/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SUDANO, M. J.; SANTOS, V. G.; TATA, A.; FERREIRA, C. R.; PASCHOAL, D. M.; MACHADO, R.; BURATINI, J.; EBERLIN, M. N.; LANDIM-ALVARENGA, F. D. C. |
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Afiliação: |
MATEUS J. SUDANO, UNESP; VANESSA G. SANTOS, University of Campinas; ALESSANDRA TATA, University of Campinas; CHRISTINA R. FERREIRA, University of Campinas; DANIELA M. PASCHOAL, UNESP; RUI MACHADO, CPPSE; JOSÉ BURATINI, UNESP; MARCOS N. EBERLIN, University of Campinas; FERNANDA D.C. LANDIM-ALVARENGA, UNESP. |
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Título: |
Phosphatidylcholine and sphingomyelin profiles vary in Bos taurus indicus and Bos taurus taurus in vitro- and in vivo-produced blastocysts. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Biology of Reproduction, v. 87, n. 6, p. 1-11, 2012. |
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DOI: |
10.1095/biolreprod.112.102897 |
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Idioma: |
Inglês |
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Conteúdo: |
Lipid droplets, subspecies (Bos taurus indicus vs. Bos taurus taurus), and in vitro culture are known to influence cryopreservation of bovine embryos. Limited information is available regarding differences in membrane lipids in embryo, such as phosphatidylcholines (PC) and sphingomyelins (SM). The objective of the present study was to compare the profiles of several PC and SM species and relate this information to cytoplasmic lipid levels present in Nellore (B. taurus indicus) and Simmental (B. taurus taurus) blastocysts produced in vitro (IVP) or in vivo (ET). Simmental and IVP embryos had more cytoplasmic lipid content than Nellore and ET embryos (n ¼ 30). Blastocysts were submitted to matrix-assisted laser desorption/ionization mass spectrometry. Differences in the PC profile were addressed by principal component analysis. The lipid species with PC (32:1) and PC (34:1) had higher ion abundances in Nellore embryos, whereas PC (34:2) was higher in Simmental embryos. IVP embryos had less abundant ions of PC (32:1), PC (34:2), and PC (36:5) compared to ET embryos. Moreover, ion abundance of PC (32:0) was higher in both Nellore and Simmental IVP embryos compared to ET embryos. Therefore, mass spectrometry profiles of PC and SM species significantly differ with regard to unsaturation level and carbon chain composition in bovine blastocysts due to subspecies and in vitro culture conditions. Because PC abundances of Nellore and Simmental embryos were distinct (34:1 vs. 34:2), as were those of IVP and ET embryos (32:0 vs. 36:5), they are potential markers of postcryopreservation embryonic survival. MenosLipid droplets, subspecies (Bos taurus indicus vs. Bos taurus taurus), and in vitro culture are known to influence cryopreservation of bovine embryos. Limited information is available regarding differences in membrane lipids in embryo, such as phosphatidylcholines (PC) and sphingomyelins (SM). The objective of the present study was to compare the profiles of several PC and SM species and relate this information to cytoplasmic lipid levels present in Nellore (B. taurus indicus) and Simmental (B. taurus taurus) blastocysts produced in vitro (IVP) or in vivo (ET). Simmental and IVP embryos had more cytoplasmic lipid content than Nellore and ET embryos (n ¼ 30). Blastocysts were submitted to matrix-assisted laser desorption/ionization mass spectrometry. Differences in the PC profile were addressed by principal component analysis. The lipid species with PC (32:1) and PC (34:1) had higher ion abundances in Nellore embryos, whereas PC (34:2) was higher in Simmental embryos. IVP embryos had less abundant ions of PC (32:1), PC (34:2), and PC (36:5) compared to ET embryos. Moreover, ion abundance of PC (32:0) was higher in both Nellore and Simmental IVP embryos compared to ET embryos. Therefore, mass spectrometry profiles of PC and SM species significantly differ with regard to unsaturation level and carbon chain composition in bovine blastocysts due to subspecies and in vitro culture conditions. Because PC abundances of Nellore and Simmental embryos were distinct (34:1 vs. 34:2), as ... Mostrar Tudo |
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Palavras-Chave: |
Cryotolerance biomarkers; Lipidomics; MALDI MS; Zebuine. |
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Thesaurus Nal: |
cryopreservation; in vitro culture; mass spectrometry; taurine. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02600naa a2200325 a 4500
001 1945818
005 2023-02-16
008 2012 bl uuuu u00u1 u #d
024 7 $a10.1095/biolreprod.112.102897$2DOI
100 1 $aSUDANO, M. J.
245 $aPhosphatidylcholine and sphingomyelin profiles vary in Bos taurus indicus and Bos taurus taurus in vitro- and in vivo-produced blastocysts.$h[electronic resource]
260 $c2012
520 $aLipid droplets, subspecies (Bos taurus indicus vs. Bos taurus taurus), and in vitro culture are known to influence cryopreservation of bovine embryos. Limited information is available regarding differences in membrane lipids in embryo, such as phosphatidylcholines (PC) and sphingomyelins (SM). The objective of the present study was to compare the profiles of several PC and SM species and relate this information to cytoplasmic lipid levels present in Nellore (B. taurus indicus) and Simmental (B. taurus taurus) blastocysts produced in vitro (IVP) or in vivo (ET). Simmental and IVP embryos had more cytoplasmic lipid content than Nellore and ET embryos (n ¼ 30). Blastocysts were submitted to matrix-assisted laser desorption/ionization mass spectrometry. Differences in the PC profile were addressed by principal component analysis. The lipid species with PC (32:1) and PC (34:1) had higher ion abundances in Nellore embryos, whereas PC (34:2) was higher in Simmental embryos. IVP embryos had less abundant ions of PC (32:1), PC (34:2), and PC (36:5) compared to ET embryos. Moreover, ion abundance of PC (32:0) was higher in both Nellore and Simmental IVP embryos compared to ET embryos. Therefore, mass spectrometry profiles of PC and SM species significantly differ with regard to unsaturation level and carbon chain composition in bovine blastocysts due to subspecies and in vitro culture conditions. Because PC abundances of Nellore and Simmental embryos were distinct (34:1 vs. 34:2), as were those of IVP and ET embryos (32:0 vs. 36:5), they are potential markers of postcryopreservation embryonic survival.
650 $acryopreservation
650 $ain vitro culture
650 $amass spectrometry
650 $ataurine
653 $aCryotolerance biomarkers
653 $aLipidomics
653 $aMALDI MS
653 $aZebuine
700 1 $aSANTOS, V. G.
700 1 $aTATA, A.
700 1 $aFERREIRA, C. R.
700 1 $aPASCHOAL, D. M.
700 1 $aMACHADO, R.
700 1 $aBURATINI, J.
700 1 $aEBERLIN, M. N.
700 1 $aLANDIM-ALVARENGA, F. D. C.
773 $tBiology of Reproduction$gv. 87, n. 6, p. 1-11, 2012.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| 8. |  | PASSAMANI, F. R. F.; LOPES, N. A.; PEREIRA, G. E.; PRADO, G.; BATISTA, L. R. Aspergillus section Nigri in grapes cultivated in the tropical winery region of Brazil. Food and Public Health, Rosemead, v. 2, n. 6, p. 276-280, nov. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Semiárido; Embrapa Uva e Vinho. |
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| 11. | | PRADO, G.; OLIVEIRA, M. S.; FERREIRA, S. O.; CORREA, T. B. S.; AFFONSO, B. R. R. Ocorrencia natural de desoxinivalenol e toxina T-2 em milho pos-colheita, safra 1996. In: CONGRESSO BRASILEIRO DE CIENCIA E TECNOLOGIA DE ALIMENTOS, 16., 1998, Rio de Janeiro, RJ. Anais... Rio de Janeiro: SBCTA, 1998. v.2, p. 955-957.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 12. | | PRADO, G.; TERRA, M.; BATISTA, L. R.; LIMA, L. C. de O.; PEREIRA, G. E. Ocratoxina A em vinhos e sucos de uva elaborados no Vale do São Francisco. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 22., 2010, Salvador. Ciência e tecnologia de alimentos: potencialidades, desafios e inovações. [S.l.]: SBCTA, 2010. p. 160. 1 CD-ROM. Resumo.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 14. | | LOPES, T. R.; PRADO, G. do; ZOLIN, C. A.; PAULINO, J.; ZAIATZ, A. P. S. R. Estimativa e regionalização de vazões mínimas de referência Q7,10 e Q95% para Bacia do Rio Ivaí (PR) - como subsidio para conhecimento da disponibilidade hídrica. In: JORNADA CIENTÍFICA DA EMBRAPA AGROSSILVIPASTORIL, 4., 2015, Sinop. Resumos... Brasília, DF: Embrapa, 2015. p. 103-106| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 17. | | PASSAMANI, F. R. F.; LOPES, N. A.; BATISTA, L. R.; PEREIRA, G. E.; PRADO, G. Gênero Aspergillus seção Nigri em uvas e sementes da variedade tempranillo cultivada no Vale do São Francisco, região vitivinícola tropical do Brasil. In: CONGRESO LATINOAMERICANO DE VITICULTURA Y ENOLOGIA, 13., 2011, Santiago do Chile. Anais... Santiago: Asociación Nacional de Ingenieros Agrónomos Enólogos de Chile, 2011. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 18. | | CORREA, T. B. S.; AFFONSO, B. R.; FARIAS, A. X.; ROBBS, C. F.; PRADO, G. Deteccao e quantificacao de micotoxinas sintetizadas por Fusarium spp. em milho produzido no estado do Parana. In: CONGRESSO BRASILEIRO DE CIENCIA E TECNOLOGIA DE ALIMENTOS, 15., 1996, Pocos de Caldas, MG. Resumos... Pocos de Caldas: SBCTA, 1996. p. 81, ref. 220.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 20. | | PRADO, G. S.; PINHEIRO, T. T.; FARIA, J. C. de; VIANELLO, R. P. Edição de genoma via non-homologous end joining (NHEJ) e ribonucleoproteínas (RNP). In: MOLINARI, H. B. C.; VIEIRA, L. R.; SILVA, N. V. e; PRADO, G. S.; LOPES FILHO, J. H. (Ed.). Tecnologia CRISPR na edição genômica de plantas: biotecnologia aplicada à agricultura. Brasília, DF: Embrapa, 2020. p. 49-90. Cap. 2.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 55 | |
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