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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Ocidental. Para informações adicionais entre em contato com cpaa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
10/09/2012 |
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Data da última atualização: |
12/09/2012 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CHEN, S. X.; ALMEIDA, F. F. L.; ANDERSSON, E.; TARANGER, G. L.; SCHMIDT, R.; SCHULZ, R. W.; BOGERD, J. |
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Afiliação: |
Shi X. Chen, State Key Laboratory of Marine Environmental Science, Xiamen University; FERNANDA LOUREIRO ALMEIDA OSULLIVAN, CPAA; Eva Andersson, Research Group Reproduction and Growth, Institute of Marine Research; Geir Lasse Taranger, Research Group Reproduction and Growth, Institute of Marine Research; Ruben Schmidt, Department of Biology, Faculty of Science, Utrecht University; Rüdiger W. Schulz, Department of Biology, Faculty of Science, Utrecht University; Jan Bogerd, Department of Biology, Faculty of Science, Utrecht University. |
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Título: |
Cloning, pharmacological characterization and expression analysis of Atlantic cod (Gadus morhua, L.) nuclear progesterone receptor. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
General and Comparative Endocrinology, v. 179, p. 71-77, 2012. |
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DOI: |
http://dx.doi.org/10.1016/j.ygcen.2012.07.022 |
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Idioma: |
Inglês |
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Conteúdo: |
To better understand the role(s) of progesterone in fish spermatogenesis, we cloned the nuclear progesterone receptor (Pgr) of Atlantic cod. The open-reading frame of the cod pgr consists of 2076 bp, coding for a 691-amino acids-long protein that shows the highest similarity with other piscine Pgr proteins. Functional characterization of the receptor expressed in mammalian cells revealed that the cod Pgr exhibited progesterone-specific, dose-dependent induction of reporter gene expression, with 17a,20bdihydroxy- 4-pregnen-3-one (DHP), a typical piscine progesterone, showing the highest potency in activating the receptor. During ontogenesis, the pgr mRNA was undetectable in embryo?s 24 h after fertilization, but became detectable 4 days after fertilization. During the larval stage, the expression levels increased steadily with the development of the larvae. In adult fish, pgr was predominantly expressed in gonads of both sexes. During the onset of puberty, testicular pgr transcript levels started to increase during rapid spermatogonial proliferation, and peaked when spermiation started. In situ hybridization studies using testis tissue during the rapid growth phase containing all germ cell stages indicated that in cod, pgr mRNA is predominantly located in Sertoli cells that are in contact with proliferating spermatogonia. Taken together, our data suggests that the Pgr is involved in mediating progestagen stimulation of the mitotic expansion of spermatogonia, and in processes associated with the spermiation/spawning period in Atlantic cod. MenosTo better understand the role(s) of progesterone in fish spermatogenesis, we cloned the nuclear progesterone receptor (Pgr) of Atlantic cod. The open-reading frame of the cod pgr consists of 2076 bp, coding for a 691-amino acids-long protein that shows the highest similarity with other piscine Pgr proteins. Functional characterization of the receptor expressed in mammalian cells revealed that the cod Pgr exhibited progesterone-specific, dose-dependent induction of reporter gene expression, with 17a,20bdihydroxy- 4-pregnen-3-one (DHP), a typical piscine progesterone, showing the highest potency in activating the receptor. During ontogenesis, the pgr mRNA was undetectable in embryo?s 24 h after fertilization, but became detectable 4 days after fertilization. During the larval stage, the expression levels increased steadily with the development of the larvae. In adult fish, pgr was predominantly expressed in gonads of both sexes. During the onset of puberty, testicular pgr transcript levels started to increase during rapid spermatogonial proliferation, and peaked when spermiation started. In situ hybridization studies using testis tissue during the rapid growth phase containing all germ cell stages indicated that in cod, pgr mRNA is predominantly located in Sertoli cells that are in contact with proliferating spermatogonia. Taken together, our data suggests that the Pgr is involved in mediating progestagen stimulation of the mitotic expansion of spermatogonia, and in processes ... Mostrar Tudo |
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Palavras-Chave: |
Atlantic cod; Nuclear progesterone receptor. |
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Thesaurus Nal: |
spermatogenesis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02333naa a2200241 a 4500
001 1933292
005 2012-09-12
008 2012 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1016/j.ygcen.2012.07.022$2DOI
100 1 $aCHEN, S. X.
245 $aCloning, pharmacological characterization and expression analysis of Atlantic cod (Gadus morhua, L.) nuclear progesterone receptor.
260 $c2012
520 $aTo better understand the role(s) of progesterone in fish spermatogenesis, we cloned the nuclear progesterone receptor (Pgr) of Atlantic cod. The open-reading frame of the cod pgr consists of 2076 bp, coding for a 691-amino acids-long protein that shows the highest similarity with other piscine Pgr proteins. Functional characterization of the receptor expressed in mammalian cells revealed that the cod Pgr exhibited progesterone-specific, dose-dependent induction of reporter gene expression, with 17a,20bdihydroxy- 4-pregnen-3-one (DHP), a typical piscine progesterone, showing the highest potency in activating the receptor. During ontogenesis, the pgr mRNA was undetectable in embryo?s 24 h after fertilization, but became detectable 4 days after fertilization. During the larval stage, the expression levels increased steadily with the development of the larvae. In adult fish, pgr was predominantly expressed in gonads of both sexes. During the onset of puberty, testicular pgr transcript levels started to increase during rapid spermatogonial proliferation, and peaked when spermiation started. In situ hybridization studies using testis tissue during the rapid growth phase containing all germ cell stages indicated that in cod, pgr mRNA is predominantly located in Sertoli cells that are in contact with proliferating spermatogonia. Taken together, our data suggests that the Pgr is involved in mediating progestagen stimulation of the mitotic expansion of spermatogonia, and in processes associated with the spermiation/spawning period in Atlantic cod.
650 $aspermatogenesis
653 $aAtlantic cod
653 $aNuclear progesterone receptor
700 1 $aALMEIDA, F. F. L.
700 1 $aANDERSSON, E.
700 1 $aTARANGER, G. L.
700 1 $aSCHMIDT, R.
700 1 $aSCHULZ, R. W.
700 1 $aBOGERD, J.
773 $tGeneral and Comparative Endocrinology$gv. 179, p. 71-77, 2012.
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Embrapa Amazônia Ocidental (CPAA) |
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| Registros recuperados : 70 | |
| 10. |  | RAMOS, A. R.; MARCELLINO, L. H.; GANDER, E. S. Um método para extração de DNA de cacau (Theobroma cacao). In: CONGRESSO NACIONAL DE BOTÂNICA, 54.; REUNIÃO DE BOTÂNICOS DA AMAZÔNIA, 3., 2003, Belém. Desafios da Botânica no Novo Milênio, Sistematização e Conservação da Diversidade Vegetal: [resumos]. Belém: Sociedade Botânica do Brasil: Universidade Federal Rural da Amazônia: Museu Emílio Goeldi: Embrapa Amazônia Oriental, 2003. não paginado.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 16. | | LÜDKE, L. F.; SOUSA, L. C. A.; GANDER, E. S.; MARCELLINO, L. H. Análise da expressão gene Opaque-2 de milheto (Pennisetum glaucum) e isolamento do promotor. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. Resumo 020. p. 60.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | RAMOS, A. R.; TEIXEIRA, J. B.; MARCELLINO, L. H.; GANDER, E. S. Análises protéicas em Calli de cacau desafiados com esporos de Crinipellis perniciosa. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 10., 2005, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2005. p. 46.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 18. | | PIRES, M. V.; RAMOS, A. R.; GANDER, E. S.; MARCELLINO, L. H. Construção de um vetor para estudo do promotor do gene "OPAQUE-2-LIKE" de milheto. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 9., 2004, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2004. p. 56.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 19. | | MARQUES, J. P. E.; LUDKE, L. F.; GANDER, E. S.; MARCELLINO, L. H. Clonagem do gene EF1-alfa de milheto (Pennisetum glaucum). In : ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 12., 2007, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. p. 60.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | RAMOS, A. R.; GANDER, E. S.; MARTINS, N. F.; TOGAWA, R.; MARCELLINO, L. H. Clonagem de um gene do tipo osmotina em cacau (Theobroma cacao L.). In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 9., 2004, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2004. p. 51.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 70 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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