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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
31/03/2012 |
Data da última atualização: |
31/03/2012 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
TRANT, C. G. M. C.; LACERDA, T. L. S.; CARVALHO, N. B.; AZEVEDO, V.; ROSINHA, G. M. S.; SALCEDO, S. P.; GORVEL, J. P.; OLIVEIRA, S. C. |
Afiliação: |
Cyntia G. M. C. Trant, Department of Biochemistry and Immunology - UFMG; Thais L. S. Lacerda, Department of Biochemistry and Immunology - UFMG; Natalia B. Carvalho, Department of Biochemistry and Immunology - UFMG; Vasco Azevedo, Department of General Biology - UFMG; GRACIA MARIA SOARES ROSINHA, CNPGC; Suzana P. Salcedo, Centre d’Immunologie de Marseille-Luminy, Aix Marseille Universite´, Faculte´ de Sciences de Luminy, Marseille, France INSERM, U631, Marseille, France5; and CNRS, UMR6102, Marseille, France; Jean-Pierre Gorvel, Centre d’Immunologie de Marseille-Luminy, Aix Marseille Universite´, Faculte´ de Sciences de Luminy, Marseille, France INSERM, U631, Marseille, France5; and CNRS, UMR6102, Marseille, France; Sergio C. Oliveira, Department of Biochemistry and Immunology - UFMG. |
Título: |
The Brucella abortus phosphoglycerate kinase mutant is highly attenuated and induces protection superior to that of vaccine strain 19 in immunocompromised and immunocompetent mice. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Infection and Immunity, v. 78, n.5, p. 2283-2291, May 2010. |
Idioma: |
Inglês |
Conteúdo: |
Brucella abortus is a facultative intracellular bacterial pathogen that causes abortion in domestic animals and undulant fever in humans. The mechanism of virulence of Brucella spp. is not yet fully understood. Therefore, it is crucial to identify new molecules that can function as virulence factors to better understand the host-pathogen interplay. Herein, we identified the gene encoding the phosphoglycerate kinase (PGK) of B. abortus strain 2308. To test the role of PGK in Brucella pathogenesis, a pgk deletion mutant was constructed. Replacement of the wild-type pgk by recombination was demonstrated by Southern and Western blot analyses. The B. abortus pgk mutant strain exhibited extreme attenuation in bone marrow-derived macrophages and in vivo in BALB/c, C57BL/6, 129/Sv, and interferon regulatory factor-1 knockout (IRF-1 KO) mice. Additionally, at 24 h postinfection the pgk mutant was not found within the same endoplasmic reticulum-derived compartment as the wild-type bacteria, but, instead, over 60% of Brucella-containing vacuoles (BCVs) retained the late endosomal/lysosomal marker LAMP1. Furthermore, the B. abortus pgk deletion mutant was used as a live vaccine. Challenge experiments revealed that the pgk mutant strain induced protective immunity in 129/Sv or IRF-1 KO mice that was superior to the protection conferred by commercial strain 19 or RB51. Finally, the results shown here demonstrated that Brucella PGK is critical for full bacterial virulence and that a pgk mutant may serve as a potential vaccine candidate in future studies. MenosBrucella abortus is a facultative intracellular bacterial pathogen that causes abortion in domestic animals and undulant fever in humans. The mechanism of virulence of Brucella spp. is not yet fully understood. Therefore, it is crucial to identify new molecules that can function as virulence factors to better understand the host-pathogen interplay. Herein, we identified the gene encoding the phosphoglycerate kinase (PGK) of B. abortus strain 2308. To test the role of PGK in Brucella pathogenesis, a pgk deletion mutant was constructed. Replacement of the wild-type pgk by recombination was demonstrated by Southern and Western blot analyses. The B. abortus pgk mutant strain exhibited extreme attenuation in bone marrow-derived macrophages and in vivo in BALB/c, C57BL/6, 129/Sv, and interferon regulatory factor-1 knockout (IRF-1 KO) mice. Additionally, at 24 h postinfection the pgk mutant was not found within the same endoplasmic reticulum-derived compartment as the wild-type bacteria, but, instead, over 60% of Brucella-containing vacuoles (BCVs) retained the late endosomal/lysosomal marker LAMP1. Furthermore, the B. abortus pgk deletion mutant was used as a live vaccine. Challenge experiments revealed that the pgk mutant strain induced protective immunity in 129/Sv or IRF-1 KO mice that was superior to the protection conferred by commercial strain 19 or RB51. Finally, the results shown here demonstrated that Brucella PGK is critical for full bacterial virulence and that a pgk mu... Mostrar Tudo |
Thesagro: |
Brucella Abortus; Sanidade Animal. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02342naa a2200229 a 4500 001 1921301 005 2012-03-31 008 2010 bl uuuu u00u1 u #d 100 1 $aTRANT, C. G. M. C. 245 $aThe Brucella abortus phosphoglycerate kinase mutant is highly attenuated and induces protection superior to that of vaccine strain 19 in immunocompromised and immunocompetent mice.$h[electronic resource] 260 $c2010 520 $aBrucella abortus is a facultative intracellular bacterial pathogen that causes abortion in domestic animals and undulant fever in humans. The mechanism of virulence of Brucella spp. is not yet fully understood. Therefore, it is crucial to identify new molecules that can function as virulence factors to better understand the host-pathogen interplay. Herein, we identified the gene encoding the phosphoglycerate kinase (PGK) of B. abortus strain 2308. To test the role of PGK in Brucella pathogenesis, a pgk deletion mutant was constructed. Replacement of the wild-type pgk by recombination was demonstrated by Southern and Western blot analyses. The B. abortus pgk mutant strain exhibited extreme attenuation in bone marrow-derived macrophages and in vivo in BALB/c, C57BL/6, 129/Sv, and interferon regulatory factor-1 knockout (IRF-1 KO) mice. Additionally, at 24 h postinfection the pgk mutant was not found within the same endoplasmic reticulum-derived compartment as the wild-type bacteria, but, instead, over 60% of Brucella-containing vacuoles (BCVs) retained the late endosomal/lysosomal marker LAMP1. Furthermore, the B. abortus pgk deletion mutant was used as a live vaccine. Challenge experiments revealed that the pgk mutant strain induced protective immunity in 129/Sv or IRF-1 KO mice that was superior to the protection conferred by commercial strain 19 or RB51. Finally, the results shown here demonstrated that Brucella PGK is critical for full bacterial virulence and that a pgk mutant may serve as a potential vaccine candidate in future studies. 650 $aBrucella Abortus 650 $aSanidade Animal 700 1 $aLACERDA, T. L. S. 700 1 $aCARVALHO, N. B. 700 1 $aAZEVEDO, V. 700 1 $aROSINHA, G. M. S. 700 1 $aSALCEDO, S. P. 700 1 $aGORVEL, J. P. 700 1 $aOLIVEIRA, S. C. 773 $tInfection and Immunity$gv. 78, n.5, p. 2283-2291, May 2010.
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Embrapa Gado de Corte (CNPGC) |
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Biblioteca(s): |
Embrapa Solos. |
Data corrente: |
03/03/1997 |
Data da última atualização: |
19/05/2022 |
Autoria: |
MELO FILHO, H. F. R. de; MEDEIROS, L. A. R.; JACOMINE, P. K. T. |
Afiliação: |
HERACLIO FERNANDES R DE MELO F, SNLCS; LUIZ ALBERTO REGUEIRA MEDEIROS, SNLCS; PAULO KLINGER TITO JACOMINE, CNPS. |
Título: |
Levantamento detalhado dos solos da área da UEPAE de Teresina, PI. |
Ano de publicação: |
1980 |
Fonte/Imprenta: |
Rio de Janeiro: EMBRAPA-SNLCS, 1980. |
Páginas: |
154 p. |
Série: |
(EMBRAPA-SNLCS. Boletim técnico, 69). |
Idioma: |
Português |
Notas: |
Acompanha 1 mapa, p&b. Escala 1:8.000. |
Conteúdo: |
A equipe de pedologia da Coordenadoria do Nordeste do Servico Nacional de Levantamento e Conservacao de Solos-EMBRAPA, atendendo a solicitacao da UEPAE de Teresina-EMBRAPA, efetuou o levantamento pedologico da area da sede desta UEPAE, situada nas proximidades de Teresina, e com uma superficie de 320 ha. A realizacao deste levantamento teve por objetivo a identificacao e estudo das diversas unidades de solos existentes na area, compreendendo investigacao das caracteristicas morfologicas, fisicas e quimicas, assim como a extensao das mesmas tendo em vista a sua utilizacao. O presente trabalho tem como finalidade fornecer tambem, subsidios basicos para planejamento e execusao de experimentos agricolas, visando obter resultados que possam ser extrapolados principalmente para areas de unidades de solos semelhantes, existentes em outras regioes, em todo o Estado do Piaui. |
Palavras-Chave: |
Classificação; Mapeamento; Piauí; Recursos naturais; Solos; Teresina; UEPAE. |
Thesagro: |
Classificação do Solo; Clima; Geologia; Levantamento; Reconhecimento do Solo; Relevo; Solo; Vegetação. |
Thesaurus NAL: |
Soil; Soil classification; Soil surveys. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/62779/1/CNPS-BOL.-TEC.-69-80.pdf
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Marc: |
LEADER 01895nam a2200385 a 4500 001 1336005 005 2022-05-19 008 1980 bl uuuu 00u1 u #d 100 1 $aMELO FILHO, H. F. R. de 245 $aLevantamento detalhado dos solos da área da UEPAE de Teresina, PI. 260 $aRio de Janeiro: EMBRAPA-SNLCS$c1980 300 $a154 p. 490 $a(EMBRAPA-SNLCS. Boletim técnico, 69). 500 $aAcompanha 1 mapa, p&b. Escala 1:8.000. 520 $aA equipe de pedologia da Coordenadoria do Nordeste do Servico Nacional de Levantamento e Conservacao de Solos-EMBRAPA, atendendo a solicitacao da UEPAE de Teresina-EMBRAPA, efetuou o levantamento pedologico da area da sede desta UEPAE, situada nas proximidades de Teresina, e com uma superficie de 320 ha. A realizacao deste levantamento teve por objetivo a identificacao e estudo das diversas unidades de solos existentes na area, compreendendo investigacao das caracteristicas morfologicas, fisicas e quimicas, assim como a extensao das mesmas tendo em vista a sua utilizacao. O presente trabalho tem como finalidade fornecer tambem, subsidios basicos para planejamento e execusao de experimentos agricolas, visando obter resultados que possam ser extrapolados principalmente para areas de unidades de solos semelhantes, existentes em outras regioes, em todo o Estado do Piaui. 650 $aSoil 650 $aSoil classification 650 $aSoil surveys 650 $aClassificação do Solo 650 $aClima 650 $aGeologia 650 $aLevantamento 650 $aReconhecimento do Solo 650 $aRelevo 650 $aSolo 650 $aVegetação 653 $aClassificação 653 $aMapeamento 653 $aPiauí 653 $aRecursos naturais 653 $aSolos 653 $aTeresina 653 $aUEPAE 700 1 $aMEDEIROS, L. A. R. 700 1 $aJACOMINE, P. K. T.
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