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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
22/03/2012 |
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Data da última atualização: |
22/03/2012 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FONSECA JÚNIOR, A. A.; MAGALHÃES, C. G.; SALES, E. B.; D'AMBROS, R. M.; ZANELLA, J. R. C.; HEINEMANN, M. B.; LEITE, R. C.; REIS, J. K. P. dos. |
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Afiliação: |
ANTONIO AUGUSTO FONSECA JÚNIOR, LANAGRO; CRISTINA GONÇALVES MAGALHÃES, LANAGRO; ERICA BRAVO SALES, LANAGRO; REGIA MARIA D'AMBROS, CEDISA; JANICE REIS CIACCI ZANELLA, CNPSA; MARCOS BRYAN HEINEMANN, UFMG; ROMULO CERQUEIRA LEITE, UFMG; JENNER KARLISSON PIMENTA DOS REIS, UFMG. |
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Título: |
Genotyping of the Pseudorabies vírus by multiplex PCR followed by restriction enzyme analysis. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
ISRN Microbiology, v. 2011. Doi:10.5402/2011/458294. |
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Idioma: |
Inglês |
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Notas: |
Projeto/Plano de Ação: 03.09.00.046. |
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Conteúdo: |
Suid herpesvirus 1 (SuHV-1) is the causative agent of Aujeszky?s disease. The infectious agent has only one serotype, but it was classified by restriction enzyme analysis of the whole genome into four genotypes, named I to IV. The aim of this study was to standardize a rapid method for genotyping SuHV-1 without virus isolation, using a multiplex-PCR followed by enzymatic restriction analysis. The complete genome of the virus was analyzed in silico to determine the restriction sites for the enzyme BamHI. Primers were designed to flank sites with emphasis on certain points of differentiation of genotypes. The standard PCRs were able to detect the SuHV-1 and also to differentiate genotypes from brain tissue of infected pigs. The BamHI-PCR is a rapid, practical, and sensitive way to genotype SuHV-1. |
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Palavras-Chave: |
PCR. |
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Thesagro: |
Genótipo; Vírus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01560naa a2200253 a 4500
001 1919948
005 2012-03-22
008 2011 bl uuuu u00u1 u #d
100 1 $aFONSECA JÚNIOR, A. A.
245 $aGenotyping of the Pseudorabies vírus by multiplex PCR followed by restriction enzyme analysis.$h[electronic resource]
260 $c2011
500 $aProjeto/Plano de Ação: 03.09.00.046.
520 $aSuid herpesvirus 1 (SuHV-1) is the causative agent of Aujeszky?s disease. The infectious agent has only one serotype, but it was classified by restriction enzyme analysis of the whole genome into four genotypes, named I to IV. The aim of this study was to standardize a rapid method for genotyping SuHV-1 without virus isolation, using a multiplex-PCR followed by enzymatic restriction analysis. The complete genome of the virus was analyzed in silico to determine the restriction sites for the enzyme BamHI. Primers were designed to flank sites with emphasis on certain points of differentiation of genotypes. The standard PCRs were able to detect the SuHV-1 and also to differentiate genotypes from brain tissue of infected pigs. The BamHI-PCR is a rapid, practical, and sensitive way to genotype SuHV-1.
650 $aGenótipo
650 $aVírus
653 $aPCR
700 1 $aMAGALHÃES, C. G.
700 1 $aSALES, E. B.
700 1 $aD'AMBROS, R. M.
700 1 $aZANELLA, J. R. C.
700 1 $aHEINEMANN, M. B.
700 1 $aLEITE, R. C.
700 1 $aREIS, J. K. P. dos
773 $tISRN Microbiology$gv. 2011. Doi:10.5402/2011/458294.
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Embrapa Suínos e Aves (CNPSA) |
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| Registros recuperados : 9 | |
| 3. |  | BAIER, A. C.; SOMERS, D. J.; GUSTAFSON, J. P. Hydroponic evaluation of aluminum tolerance in triticale, wheat, and rye. In: MONIZ, A. C.; FURLANI, A. M. C.; SCHAFFERT, R. E.; FAGERIA, N. K.; ROSOLEM, C. A.; CANTARELLA, H. (Ed.). Plant-soil interactions at low pH: sustainable agriculture and Forestry Production. Campinas: Brazilian Soil Science Society, 1997. p. 231. Proceedings of the IV International Symposium on Plant-Soil Interactions at Low pH, Belo Horizonte, 1996. Abstract P24.| Biblioteca(s): Embrapa Trigo. |
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| 5. | | ANDOW, D. A.; SOMERS, D. A.; AMUGUNE, N.; ARAGÃO, F. J. L.; GHOSH, K.; GUDU, S.; MAGIRI, E.; MOAR, W. J.; NJIHIA, S.; OSIR, E. Transgene locus structure and expression of BT maize. In: HILBECK, A.; ANDOW, D. A. Environmental risk assessment of genetically modified organisms: a case study of Bt Maize in Kenya. Wallingford, UK: CABI Publishing, 2004. 281 p. (Environmental risk assessment of genetically modified organisms series, v. 1). p. 83-116.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | COMEAU, A.; LANGEVIN, F.; CAETANO, V. da R.; HEBER, S.; SAVARD, M. E.; VOLDENG, H.; FEDAK, G.; DION, Y.; RIOUX, S.; GILBERT, J.; SOMERS, D.; MARTIN, R. A. A sistemic approach for the development of FHB resistant germplasm accelerates. Cereal research communications, Hungria, v. 36, n. 6, p. 5-9, 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 7. | | CAMEAU, A.; LANGEVIN, F.; CAETANO, V. da R.; HABER, S.; SAVARD, M. E.; VOLDENG, H.; FEDAK, G.; DION, Y.; RIOUX, S.; GILBERT, J.; SOMERS, D.; MARTIN, R. A. A systemic approach for the development of FHB resistant germplasm accelerates genetic progress. Cereal Research Communications, Hungria, v. 36, p. 6-9, 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 8. | | COMEAU, A.; LANGEVIN, F.; CAETANO, V. da R.; HABER, S.; SAVARD, M. E.; VOLDENG, H.; FEDAK, G.; DION, Y.; RIOUX, S.; GILBERT, J.; SOMERS, D.; MARTIN, R. A.; SCHEEREN, P. L. A different path to the summit of fusarium head blight resistance in wheat: developing germplasm with a systemic approach. In: EUROPEAN FUSARIUM SEMINAR 'FUSARIUM - MYCOTOXINS, TAXONOMY, PATHOGENICITY AND HOST RESISTANCE', 11., 2010, Radzików. Book of Abstracts Radzików: IHAR, 2010. p. 75-76| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 9. | | GROSSI-DE-SÁ, M. F.; LUCENA, W.; SOUZA, M. L.; NEPOMUCENO, A. L.; OSIR, E. O.; AMUGUNE, N.; HOA, T. T. C.; HAI, T. N.; SOMERS, D. A.; ROMANO, E. Transgene expression and locus structure of Bt cotton. In: HILBECK, A.; ANDOW, D. A.; FONTES, E. M. G.; KAPUSCINSKI, A. R.; SCHEI, P. J. (Ed.). Environmental risk assessment of genetically modified organisms: methodologies for assessing Bt cotton in Brazil. Wallingford, UK: CABI Publishing, 2006. v. 2. p. 93-107. (Environmental risk assessment of genetically modified organisms series, v. 2).| Biblioteca(s): Embrapa Algodão; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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| Registros recuperados : 9 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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