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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
07/03/2012 |
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Data da última atualização: |
21/10/2015 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; LIMA, M. R. de; NICIURA, S. C. M.; GARCIA, J. M. |
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Afiliação: |
UNESP/JABOTICABAL; UNESP/JABOTICABAL; UNESP/JABOTICABAL; UNESP/JABOTICABAL; SIMONE CRISTINA MEO NICIURA, CPPSE; UNESP/JABOTICABAL. |
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Título: |
Effects of demecolcine on the meiotic cell-cycle and microtubular kinetics of activated bovine oocytes submitted to chemical enucleation. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: ANUAL CONFERENCE OF INTERNATIONAL EMBRYO TRANSFER SOCIETY, 24., Phoenix, Arizona. Phoenix: CSIRO, 2012 |
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Páginas: |
p. 119 |
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Idioma: |
Português |
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Conteúdo: |
Enucleation in traditional nuclear transfer (NT) is an invasive procedure for which alternative protocols have been sought. The present study was designed to explore the time course effects of demecolcine, a microtubule-depolymerizing agent, in bovine-activated oocytes submitted to induced chemical enucleation. For that purpose, after 26 h of in vitro maturation, the oocytes were parthenogenetically activated (5 mMionomycin for 5 min and 10 mgmL 1 cycloheximide for 4 h) and treated with demecolcine (0.05 mgmL 1 for 2 h) 2 h after activation. Three groups were established: control (untreated oocytes), activated (oocytes exposed to activation) and deme (oocytes activated and treated with demecolcine). Then the nuclear and microtubular dynamics of the oocytes were evaluated by immunofluorescence microscopy of tubulin and chromatin (Liu et al. 1998 Biol. Reprod. 59, 537?545). In each one of 3 replicates, 15 to 30 oocytes were evaluated per group. Oocytes were classified according to microtubule (MT) patterns as follows: present (evident MT), reduced (MT with reduced density), or absent. The results in percentage were submitted toANOVAand means were compared by Tukey test, with a significance level of 5%. Effects of activation were observed after 2 h, when higher rates of oocytes presenting second polar body (2nd PB) extrusion were observed in the groups activated and deme (49.3% in both groups) compared with control (1.7%). At the end of activation treatment (4 h), the activated group presented 81.8% of oocytes with 2nd PB extrusion, whereas it was observed only in 37.8% of oocytes in the deme group. Effects of demecolcine on the microtubules initiated after only 0.5 h of treatment, when an increase (Po0.05) of oocytes with reduced MT was observed in the deme group (26%; control ? 3%; activated ? 0%). After 6 h of culture in demecolcine-free medium, the deme group displayed ,50% of oocytes with reduced MT (control ? 0%; activated ? 39%) and absence of MT in 23% of oocytes, which was superior to other groups (control ? 0%; activated ? 2%). Therefore, we detected a reduction of MT density after exposition of activated oocytes to demecolcine. However, MT were not completely absent in most of the evaluated oocytes, as previously described for bovine oocytes submitted to chemically assisted enucleation (Saraiva et al. 2009 Cloning Stem Cells 11, 141?152; Meng et al. 2011 Cell. Reprogram., in press). Apparently, there was no immediate repolymerization of MT after culture in demecolcine-free medium and this could result in negative consequences for subsequent embryo development. Moreover, demecolcine impaired the second PB extrusion during the activation process, probably due to inhibition of spindle rotation caused by the MT-disrupting drug. Nonetheless, considering the higher cytoplasmatic volume obtained with chemical enucleation and the lesser extent of injuries suffered by recipient oocytes, further studies focusing on the potential of embryo development and the quality of embryos are advisable. MenosEnucleation in traditional nuclear transfer (NT) is an invasive procedure for which alternative protocols have been sought. The present study was designed to explore the time course effects of demecolcine, a microtubule-depolymerizing agent, in bovine-activated oocytes submitted to induced chemical enucleation. For that purpose, after 26 h of in vitro maturation, the oocytes were parthenogenetically activated (5 mMionomycin for 5 min and 10 mgmL 1 cycloheximide for 4 h) and treated with demecolcine (0.05 mgmL 1 for 2 h) 2 h after activation. Three groups were established: control (untreated oocytes), activated (oocytes exposed to activation) and deme (oocytes activated and treated with demecolcine). Then the nuclear and microtubular dynamics of the oocytes were evaluated by immunofluorescence microscopy of tubulin and chromatin (Liu et al. 1998 Biol. Reprod. 59, 537?545). In each one of 3 replicates, 15 to 30 oocytes were evaluated per group. Oocytes were classified according to microtubule (MT) patterns as follows: present (evident MT), reduced (MT with reduced density), or absent. The results in percentage were submitted toANOVAand means were compared by Tukey test, with a significance level of 5%. Effects of activation were observed after 2 h, when higher rates of oocytes presenting second polar body (2nd PB) extrusion were observed in the groups activated and deme (49.3% in both groups) compared with control (1.7%). At the end of activation treatment (4 h), the activated... Mostrar Tudo |
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Palavras-Chave: |
Demecolcine; Meiotic cell cycle. |
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Thesaurus Nal: |
oocytes. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 03762nam a2200217 a 4500
001 1917825
005 2015-10-21
008 2012 bl uuuu u00u1 u #d
100 1 $aSARAIVA, N. Z.
245 $aEffects of demecolcine on the meiotic cell-cycle and microtubular kinetics of activated bovine oocytes submitted to chemical enucleation.
260 $aIn: ANUAL CONFERENCE OF INTERNATIONAL EMBRYO TRANSFER SOCIETY, 24., Phoenix, Arizona. Phoenix: CSIRO$c2012
300 $ap. 119
520 $aEnucleation in traditional nuclear transfer (NT) is an invasive procedure for which alternative protocols have been sought. The present study was designed to explore the time course effects of demecolcine, a microtubule-depolymerizing agent, in bovine-activated oocytes submitted to induced chemical enucleation. For that purpose, after 26 h of in vitro maturation, the oocytes were parthenogenetically activated (5 mMionomycin for 5 min and 10 mgmL 1 cycloheximide for 4 h) and treated with demecolcine (0.05 mgmL 1 for 2 h) 2 h after activation. Three groups were established: control (untreated oocytes), activated (oocytes exposed to activation) and deme (oocytes activated and treated with demecolcine). Then the nuclear and microtubular dynamics of the oocytes were evaluated by immunofluorescence microscopy of tubulin and chromatin (Liu et al. 1998 Biol. Reprod. 59, 537?545). In each one of 3 replicates, 15 to 30 oocytes were evaluated per group. Oocytes were classified according to microtubule (MT) patterns as follows: present (evident MT), reduced (MT with reduced density), or absent. The results in percentage were submitted toANOVAand means were compared by Tukey test, with a significance level of 5%. Effects of activation were observed after 2 h, when higher rates of oocytes presenting second polar body (2nd PB) extrusion were observed in the groups activated and deme (49.3% in both groups) compared with control (1.7%). At the end of activation treatment (4 h), the activated group presented 81.8% of oocytes with 2nd PB extrusion, whereas it was observed only in 37.8% of oocytes in the deme group. Effects of demecolcine on the microtubules initiated after only 0.5 h of treatment, when an increase (Po0.05) of oocytes with reduced MT was observed in the deme group (26%; control ? 3%; activated ? 0%). After 6 h of culture in demecolcine-free medium, the deme group displayed ,50% of oocytes with reduced MT (control ? 0%; activated ? 39%) and absence of MT in 23% of oocytes, which was superior to other groups (control ? 0%; activated ? 2%). Therefore, we detected a reduction of MT density after exposition of activated oocytes to demecolcine. However, MT were not completely absent in most of the evaluated oocytes, as previously described for bovine oocytes submitted to chemically assisted enucleation (Saraiva et al. 2009 Cloning Stem Cells 11, 141?152; Meng et al. 2011 Cell. Reprogram., in press). Apparently, there was no immediate repolymerization of MT after culture in demecolcine-free medium and this could result in negative consequences for subsequent embryo development. Moreover, demecolcine impaired the second PB extrusion during the activation process, probably due to inhibition of spindle rotation caused by the MT-disrupting drug. Nonetheless, considering the higher cytoplasmatic volume obtained with chemical enucleation and the lesser extent of injuries suffered by recipient oocytes, further studies focusing on the potential of embryo development and the quality of embryos are advisable.
650 $aoocytes
653 $aDemecolcine
653 $aMeiotic cell cycle
700 1 $aOLIVEIRA, C. S.
700 1 $aTETZNER, T. A. D.
700 1 $aLIMA, M. R. de
700 1 $aNICIURA, S. C. M.
700 1 $aGARCIA, J. M.
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Embrapa Pecuária Sudeste (CPPSE) |
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| 9. |  | BORGES, R.; KLAIC, R.; CALEGARI, R. P.; RIBEIRO, C.; FARINAS, C. S. Estudo da degradação das fibras de crisotila a partir do tratamento com ácido oxálico. In: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 4., 2019, São Carlos, SP. Ciência, inovação e mercado: anais. São Carlos, SP: Embrapa Instrumentação, 2019. Editores: Paulino Ribeiro Villas-Boas, Maria Alice Martins, Débora Marcondes Bastos Pereira Milori, Ladislau Martin Neto. SIAGRO 2019. 540 - 544| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 10. | | CALEGARI, L.; LOPES, P. J. G.; OLIVEIRA, E. de; GATTO, D. A.; STANGERLIN, D. M. Quantificação de taninos nas cascas de jurema-preta e acácia-negra. Pesquisa Florestal Brasileira, Colombo, v. 36, n. 85, p. 61-69, jan./mar. 2016.| Biblioteca(s): Embrapa Florestas. |
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| 12. | | CALEGARI, A.; HERNANI, L. C.; PITOL, C.; PRIMAVESI, O.; RESCK, D. V. S. Manejo do material orgânico. In: SALTON, J. C.; HERNANI, L. C.; FONTES, C. Z. (Org.). Sistema plantio direto: o produtor pergunta, a Embrapa responde. Brasília, DF: Embrapa Serviço de Produção de Informação; Dourados: Embrapa Agropecuária Oeste, 1998. p. 51-57. (Coleção 500 perguntas, 500 respostas).| Biblioteca(s): Embrapa Cerrados. |
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| 13. | | WUTKE, E. B.; CALEGARI, A.; WILDNER, L. do P.; AMABILE, R. F. Leguminosas alimentícias e adubos verdes. In: ALBUQUERQUE, A. C. S.; SILVA, A. G. da (Ed.). Agricultura tropical: quatro décadas de inovações tecnológicas, institucionais e políticas. Brasília, DF: Embrapa Informação Tecnológica, 2008. pt. 2, cap. 8, p. 251-274.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Cerrados. |
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| 14. | | MACEDO, R. S.; MADELLA, M.; CALEGARI, M. R.; TEIXEIRA, W. G.; MARTINS, G. C.; VIDAL-TORRADO, P. Coleção de referência de fitólitos de plantas como subsídio para identificação e classificação de fitólitos em solos. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 35., 2015, Natal. O solo e suas múltiplas funções: anais. Natal: Sociedade Brasileira de Ciência do Solo, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 15. | | MACEDO, R. S.; MADELLA, M.; CALEGARI, M. R.; TEIXEIRA, W. G.; MARTINS, G. C.; VIDAL-TORRADO, P. Coleção de referência de fitólitos de plantas como subsídio para identificação e classificação de fitólitos em solos. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 35., 2015, Natal. O solo e suas múltiplas funções: anais. Natal: Sociedade Brasileira de Ciência do Solo, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| 16. | | SCHOMBERG, H. H.; ENDALE, D. M.; CALEGARI, A.; PEIXOTO, R. dos G. T.; MIYAZAWA, M.; CABRERA, M. L. Influence of cover crops on potential nitrogen availability to succeeding crops in a Southern Piedmont soil. Biology and Fertility of Soils, Berlin, v. 42, n. 4, p. 299-307, mar. 2006.| Biblioteca(s): Embrapa Agrobiologia. |
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| 17. | | SCHOMBERG, H. H.; ENDALE, D. M.; CALEGARI, A.; PEIXOTO, R. T. dos G.; MIYAZAWA, M.; CABRERA, M. L. Influence of cover crops on potential nitrogen availability to succeeding crops in a southern Piedmont soil. Biology and Fertility of Soils, Boca Raton, v. 42, n. 4, p. 299-307, mar. 2006.| Biblioteca(s): Embrapa Agrobiologia. |
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| 18. | | MACEDO, R. S.; MADELLA, M.; CALEGARI, M. R.; TEIXEIRA, W. G.; MARTINS, G. C.; VIDAL-TORRADO, P. Fitólitos como subsídio para estudos de reconstrução da vegetação e indicador de mudanças hidrológicas em solos tropicais. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 35., 2015, Natal. O solo e suas múltiplas funções: anais. Natal: Sociedade Brasileira de Ciência do Solo, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 19. | | MACEDO, R. S.; MADELLA, M.; CALEGARI, M. R.; TEIXEIRA, W. G.; MARTINS, G. C.; VIDAL-TORRADO, P. Fitólitos como subsídio para estudos de reconstrução da vegetação e indicador de mudanças hidrológicas em solos tropicais. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 35., 2015, Natal. O solo e suas múltiplas funções: anais. Natal: Sociedade Brasileira de Ciência do Solo, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| 20. | | CALEGARI, A.; HERNANI, L. C.; PITOL, C.; PRIMAVESI, O. M. A. S. P. R.; RESCK, D. V. S. Manejo do material orgânico. In: SALON, J.C.; HERNANI, L.C.; FONTES, C.Z. (Org.). Sistema de Plantio direto: O produtor pergunta, a Embrapa responde. Brasilia : Embrapa-SPI/Dourados : Embrapa agropecuária Oeste, 1998. (Coleção 500 Perguntas 500 Respostas)| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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