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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
12/12/2011 |
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Data da última atualização: |
10/04/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, T. L. P. O.; BARROS, E. G. de; BELLATO, C. M.; HWANG, E.-Y.; CREGAN, P. B.; PASTOR-CORRALES, M. A. |
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Afiliação: |
THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF; EVERALDO G. DE BARROS, UNIVERSIDADE FEDERAL DE VIÇOSA; CLAUDIA M. BELLATO, USDA; EUN-YOUNG HWANG, USDA; PERRY B. CREGAN, USDA; MARCIAL A. PASTOR-CORRALES, USDA. |
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Título: |
Single nucleotide polymorphism discovery in common bean. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Molecular Breeding, v. 30, p. 419-428, 2012. |
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DOI: |
DOI 10.1007/s11032-011-9632-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Single nucleotide polymorphisms (SNPs) were discovered in common bean (Phaseolus vulgaris L.) via resequencing of sequence-tagged sites (STSs) developed by PCR primers previously designed to soybean shotgun and bacterial artificial chromosome (BAC) end sequences, and by primers designed to common bean genes and microsatellite flanking regions. DNA fragments harboring SNPs were identified in single amplicons from six contrasting P. vulgaris genotypes of the Andean (Jalo EEP 558, G 19833, and AND 277) and Mesoamerican (BAT 93, DOR 364, and Ruda´) gene pools. These genotypes are the parents of three common bean recombinant inbred line mapping populations. From an initial set of 1,880 PCR primer pairs tested, 265 robust STSs were obtained, which could be sequenced in each one of the six common bean genotypes. In the resulting 131,120 bp of aligned sequence, a total of 677 SNPs were identified, including 555 single-base changes (295 transitions and 260 transversions) and 122 small nucleotide insertions/deletions (indels). The frequency of SNPs was 5.16 SNPs/kb and the mean nucleotide diversity, expressed as Halushka?s theta, was 0.00226. This work represents one of the first efforts aimed at detecting SNPs in P. vulgaris. The SNPs identified should be an important resource for common bean geneticists and breeders for quantitative trait locus discovery,marker-assisted selection, and map-based cloning. These SNPS will be also useful for diversity analysis and microsynteny studies among legume species. MenosSingle nucleotide polymorphisms (SNPs) were discovered in common bean (Phaseolus vulgaris L.) via resequencing of sequence-tagged sites (STSs) developed by PCR primers previously designed to soybean shotgun and bacterial artificial chromosome (BAC) end sequences, and by primers designed to common bean genes and microsatellite flanking regions. DNA fragments harboring SNPs were identified in single amplicons from six contrasting P. vulgaris genotypes of the Andean (Jalo EEP 558, G 19833, and AND 277) and Mesoamerican (BAT 93, DOR 364, and Ruda´) gene pools. These genotypes are the parents of three common bean recombinant inbred line mapping populations. From an initial set of 1,880 PCR primer pairs tested, 265 robust STSs were obtained, which could be sequenced in each one of the six common bean genotypes. In the resulting 131,120 bp of aligned sequence, a total of 677 SNPs were identified, including 555 single-base changes (295 transitions and 260 transversions) and 122 small nucleotide insertions/deletions (indels). The frequency of SNPs was 5.16 SNPs/kb and the mean nucleotide diversity, expressed as Halushka?s theta, was 0.00226. This work represents one of the first efforts aimed at detecting SNPs in P. vulgaris. The SNPs identified should be an important resource for common bean geneticists and breeders for quantitative trait locus discovery,marker-assisted selection, and map-based cloning. These SNPS will be also useful for diversity analysis and microsynteny studies a... Mostrar Tudo |
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Thesagro: |
DNA; Feijão; Phaseolus vulgaris; Polimorfismo genético. |
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Thesaurus Nal: |
Genome; Polymerase chain reaction; Single nucleotide polymorphism. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02292naa a2200277 a 4500
001 1909254
005 2025-04-10
008 2012 bl uuuu u00u1 u #d
024 7 $aDOI 10.1007/s11032-011-9632-4$2DOI
100 1 $aSOUZA, T. L. P. O.
245 $aSingle nucleotide polymorphism discovery in common bean.
260 $c2012
520 $aSingle nucleotide polymorphisms (SNPs) were discovered in common bean (Phaseolus vulgaris L.) via resequencing of sequence-tagged sites (STSs) developed by PCR primers previously designed to soybean shotgun and bacterial artificial chromosome (BAC) end sequences, and by primers designed to common bean genes and microsatellite flanking regions. DNA fragments harboring SNPs were identified in single amplicons from six contrasting P. vulgaris genotypes of the Andean (Jalo EEP 558, G 19833, and AND 277) and Mesoamerican (BAT 93, DOR 364, and Ruda´) gene pools. These genotypes are the parents of three common bean recombinant inbred line mapping populations. From an initial set of 1,880 PCR primer pairs tested, 265 robust STSs were obtained, which could be sequenced in each one of the six common bean genotypes. In the resulting 131,120 bp of aligned sequence, a total of 677 SNPs were identified, including 555 single-base changes (295 transitions and 260 transversions) and 122 small nucleotide insertions/deletions (indels). The frequency of SNPs was 5.16 SNPs/kb and the mean nucleotide diversity, expressed as Halushka?s theta, was 0.00226. This work represents one of the first efforts aimed at detecting SNPs in P. vulgaris. The SNPs identified should be an important resource for common bean geneticists and breeders for quantitative trait locus discovery,marker-assisted selection, and map-based cloning. These SNPS will be also useful for diversity analysis and microsynteny studies among legume species.
650 $aGenome
650 $aPolymerase chain reaction
650 $aSingle nucleotide polymorphism
650 $aDNA
650 $aFeijão
650 $aPhaseolus vulgaris
650 $aPolimorfismo genético
700 1 $aBARROS, E. G. de
700 1 $aBELLATO, C. M.
700 1 $aHWANG, E.-Y.
700 1 $aCREGAN, P. B.
700 1 $aPASTOR-CORRALES, M. A.
773 $tMolecular Breeding$gv. 30, p. 419-428, 2012.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| Registros recuperados : 54 | |
| 3. |  | DELGADO, J. V.; CABELLO, A.; SERENO, J. R. B.; BARBA, C.; SERENO, F. Programa de conservacion "ex situ" de las variedades del cerdo iberico en la Provincia de Cordoba. In: CONGRESO DE LA SOCIEDAD ESPANOLA PARA LOS RECURSOS GENETICOS ANIMALES, 3.; CONGRESO IBERICO SOBRE LOS RECURSOS GENETICOS ANIMALES, 1., 1999, Lugo, Galícia. Libro de Actas... Lugo: Sociedad Espanola para los Recursos Geneticos Animales, 1999. p.38.| Biblioteca(s): Embrapa Pantanal. |
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| 4. | | DELGADO, J. V.; CABELLO, A.; SERENO, J. R. B.; BARBA, C.; SERENO, F. T. P. S. Programa de conservacion ex situ de las variedades del cerdo iberico en la provincia de cordoba. Archivos de Zootecnia, Cordoba, v.50, n.189-190, p.139-144, 2001.| Biblioteca(s): Embrapa Pantanal. |
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| 6. | | SERENO, J. R. B.; DELGADO, J. V.; SERENO, F. T. P. S.; BARBA, C.; CABELLO, A. Comparative study of Iberian pigs fertility in natural service and artificial insemination with cryopreserved or refrigerated semen. In: WORLD CONFERENCE ON ANIMAL PRODUCTION, 9.; REUNIAO DA ASSOCIACAO LATINOAMERICANA DE PRODUCAO ANIMAL, 18., 2003, Porto Alegre. Abstracts. Porto Alegre: [s.n.], 2003. nao paginado. CD-ROM.| Biblioteca(s): Embrapa Pantanal. |
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| 7. | | SERENO, J. R. B.; BARBA, C.; DELGADO, J. V.; SERENO, F. T. P. S.; CABELLO, A. Razones para la eliminación de sementales ibéricos en programas de reproducción asistida. In: Porci. Aula Veterinária - Monografias de actualidad, Madrid, n.73, p.43-52, ene., 2003.| Biblioteca(s): Embrapa Pantanal. |
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| 8. | | SERENO, J. R. B.; DELGADO, J. V.; SERENO, F. T. P. S.; BARBA, C.; CABELLO, A. Banco de semen de las variedades minoritarias del cerdo Iberico. In: CONGRESO IBEROAMERICANO DE RAZAS CRIOLLAS, 6.; SIMPOSIO IBEROAMERICANO SOBRE CONSERVACION Y UTILIZACION DE RECURSOS ZOOGENETICOS, 4., 2003, Recife. Resumenes de Ponencias y Comunicaciones. Recife: FIRC / CYTED, 2003. nao paginado.| Biblioteca(s): Embrapa Pantanal. |
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| 10. | | SERENO, J. R. B.; DELGADO, J. V.; SERENO, F. T. P. S.; BARBA, C.; CABELLO, A. Bases de la caracterización del cerdo ibérico por la aptitud sexual del macho como clave para su conservación. In: Porci. Aula Veterinária - Monografias de actualidad, Madrid, n.73, p.11-19, ene., 2003.| Biblioteca(s): Embrapa Pantanal. |
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| 11. | | SERENO, J. R. B.; BARBA, C.; DELGADO, J. V.; SERENO, F. T. P. S.; CABELLO, A. Efecto de algunos factores en la recolección de semen sobre maniquí en el cerdo ibérico. In: Porci. Aula Veterinária - Monografias de actualidad, Madrid, n.73, p.31-41, ene., 2003.| Biblioteca(s): Embrapa Pantanal. |
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| 12. | | SERENO, J. R. B.; BARBA, C.; SERENO, F. T. P. S.; CABELLO, A.; DELGADO, J. V. Estudio comparativo de la fertilidad del cerdo ibérico en monta natural e inseminación artificial utilizando semen refrigerado y congelado. In: Porci. Aula Veterinária - Monografias de actualidad, Madrid, n.73, p.61-66, ene., 2003.| Biblioteca(s): Embrapa Pantanal. |
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| 13. | | BARBA, C.; DELGADO, J. V.; SERENO, J. R. B.; CABELLO, A.; SANZ, R.; DIEGUEZ, E. Diversidad genetica intrarracial del cerdo iberico. Porci, Madrid, n.61, p.11-21, ene., 2001.| Biblioteca(s): Embrapa Pantanal. |
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| 15. | | SERENO, J. R. B.; CABELLO, A.; SERENO, F. T. P. S.; BARBA, C.; DELGADO, J. V. Protocolo de criocongelación espermática propuesto en el cerdo ibérico. In: Porci. Aula Veterinária - Monografias de actualidad, Madrid, n.73, p.53-60, ene., 2003.| Biblioteca(s): Embrapa Pantanal. |
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| 17. | | BENAVENTE, M.; SILVEIRA, C.; BARBA, C.; CAMACHO, M. E.; SERENO, J. R. B.; DELGADO, J. V. Tecnologia de la conservacion: II. programas "ex situ". Canis et Felis, Madrid, n.47, p.61-68, oct., 2000.| Biblioteca(s): Embrapa Pantanal. |
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| 18. | | SERENO, J. R. B.; SERENO, F. T. P. S.; BARBA, C.; CABELLO, A.; DELGADO, J. V. Training of Iberian pigs on mounting ONTO a DUMMY at an artificial insemination programme. In: WORLD CONFERENCE ON ANIMAL PRODUCTION, 9.; REUNIAO DA ASSOCIACAO LATINOAMERICANA DE PRODUCAO ANIMAL, 18., 2003, Porto Alegre. Abstracts. Porto Alegre: [s.n.], 2003. nao paginado. CD-ROM.| Biblioteca(s): Embrapa Pantanal. |
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| 19. | | SERENO, J. R. B.; GARFIA, B.; SERENO, F. T. P. S.; BARBA, C.; CABELLO, A.; DELGADO, J. V. Sementales porcinos ibericos en programas de inseminacion artificial: razones de eliminacion. In: CONGRESO EUROPEO DE REPRODUCCION ANIMAL, 14., 2002, Lugo. Meeting European A.I. Vets. Lugo: Xunta de Galicia: A.I. Vets, 2002. p.72-77.| Biblioteca(s): Embrapa Pantanal. |
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| 20. | | SERENO, J. R. B.; DELGADO, J. V.; SERENO, F. T. P. S.; BARBA, C.; ALEJANDRO CABELLO, A. Banco de semen das diferentes variedades do suino Iberico. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 41., 2004, Campo Grande-MS. A producao animal e a seguranca alimentar - anais. Campo Grande, MS: SBZ, 2004. CD ROM 3 p.| Biblioteca(s): Embrapa Pantanal. |
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| Registros recuperados : 54 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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