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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
07/12/2011 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LESSARD, C.; SIQUEIRA, L. G. B.; D'AMOURS, O.; SULLIVAN, R.; LECLERC, P.; PALMER, C. |
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Afiliação: |
C. LESSARD, Agriculture and Agri-Food Canada, Western College of Veterinary Medicine; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; O. D'AMOURS, Université Laval, Canada; R. SULLIVAN, Université Laval, Canada; P. LECLERC, Université Laval, Canada; C. PALMER, University of Saskatchewan, Canada. |
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Título: |
Infertility in a beef bull due to a failure in the capacitation process. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Theriogenology, v. 76, n. 5, p. 891-899, 2011. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2011.04.020 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this case report was to identify the cause of apparent idiopathic infertility in a Red Angus (beef) bull. Semen was collected by electroejaculation and submitted to a series of assays, including evaluation of sperm motility by computer-assisted sperm analysis (CASA), sperm morphology and DNA integrity, semen cryopreservation, AI, IVF, induction of the acrosome reaction, and determination of the level of sperm proteins associated with bull fertility potential. Total (92 ± 2%) and progressive (79 ± 4%) sperm motility; sperm concentration (1647 ± 429 × 10(6) sperm/mL); proportions of morphologically normal sperm (83 ± 6%) and DNA integrity (96 ± 2), and acrosome-intact sperm (64 ± 4%) exceeded minimum acceptable values. Frozen sperm had good total (58.7 ± 6.7%) and progressive (43.9 ± 9.2%) motility immediately after thawing. However, AI of 16 heifers resulted in no pregnancies and blastocyst production rate (following IVF using sperm from this infertile bull) was nearly identical to that produced using dead sperm (a control of parthenogenesis; 2 ± 2 and 2 ± 3%; respectively P < 0.05). Treatment with a calcium ionophore (A23187) failed to induce the acrosome reaction in sperm from the infertile bull (P < 0.05). Evaluation of several proteins associated with the fertility potential of bulls revealed that the level of Binder Sperm Protein-1 (BSP1), known to be associated with the capacitation process, was much greater on sperm from the infertile bull compared to that of his sire. In conclusion, we inferred that the idiopathic infertility in this bull was caused by a failure to complete the capacitation process. MenosThe objective of this case report was to identify the cause of apparent idiopathic infertility in a Red Angus (beef) bull. Semen was collected by electroejaculation and submitted to a series of assays, including evaluation of sperm motility by computer-assisted sperm analysis (CASA), sperm morphology and DNA integrity, semen cryopreservation, AI, IVF, induction of the acrosome reaction, and determination of the level of sperm proteins associated with bull fertility potential. Total (92 ± 2%) and progressive (79 ± 4%) sperm motility; sperm concentration (1647 ± 429 × 10(6) sperm/mL); proportions of morphologically normal sperm (83 ± 6%) and DNA integrity (96 ± 2), and acrosome-intact sperm (64 ± 4%) exceeded minimum acceptable values. Frozen sperm had good total (58.7 ± 6.7%) and progressive (43.9 ± 9.2%) motility immediately after thawing. However, AI of 16 heifers resulted in no pregnancies and blastocyst production rate (following IVF using sperm from this infertile bull) was nearly identical to that produced using dead sperm (a control of parthenogenesis; 2 ± 2 and 2 ± 3%; respectively P < 0.05). Treatment with a calcium ionophore (A23187) failed to induce the acrosome reaction in sperm from the infertile bull (P < 0.05). Evaluation of several proteins associated with the fertility potential of bulls revealed that the level of Binder Sperm Protein-1 (BSP1), known to be associated with the capacitation process, was much greater on sperm from the infertile bull compared to ... Mostrar Tudo |
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Palavras-Chave: |
Absence of fertilization; Bovine infertility; Capacitation; Sperm morphology. |
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Thesaurus Nal: |
sperm motility. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02419naa a2200253 a 4500
001 1908898
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2011.04.020$2DOI
100 1 $aLESSARD, C.
245 $aInfertility in a beef bull due to a failure in the capacitation process.$h[electronic resource]
260 $c2011
520 $aThe objective of this case report was to identify the cause of apparent idiopathic infertility in a Red Angus (beef) bull. Semen was collected by electroejaculation and submitted to a series of assays, including evaluation of sperm motility by computer-assisted sperm analysis (CASA), sperm morphology and DNA integrity, semen cryopreservation, AI, IVF, induction of the acrosome reaction, and determination of the level of sperm proteins associated with bull fertility potential. Total (92 ± 2%) and progressive (79 ± 4%) sperm motility; sperm concentration (1647 ± 429 × 10(6) sperm/mL); proportions of morphologically normal sperm (83 ± 6%) and DNA integrity (96 ± 2), and acrosome-intact sperm (64 ± 4%) exceeded minimum acceptable values. Frozen sperm had good total (58.7 ± 6.7%) and progressive (43.9 ± 9.2%) motility immediately after thawing. However, AI of 16 heifers resulted in no pregnancies and blastocyst production rate (following IVF using sperm from this infertile bull) was nearly identical to that produced using dead sperm (a control of parthenogenesis; 2 ± 2 and 2 ± 3%; respectively P < 0.05). Treatment with a calcium ionophore (A23187) failed to induce the acrosome reaction in sperm from the infertile bull (P < 0.05). Evaluation of several proteins associated with the fertility potential of bulls revealed that the level of Binder Sperm Protein-1 (BSP1), known to be associated with the capacitation process, was much greater on sperm from the infertile bull compared to that of his sire. In conclusion, we inferred that the idiopathic infertility in this bull was caused by a failure to complete the capacitation process.
650 $asperm motility
653 $aAbsence of fertilization
653 $aBovine infertility
653 $aCapacitation
653 $aSperm morphology
700 1 $aSIQUEIRA, L. G. B.
700 1 $aD'AMOURS, O.
700 1 $aSULLIVAN, R.
700 1 $aLECLERC, P.
700 1 $aPALMER, C.
773 $tTheriogenology$gv. 76, n. 5, p. 891-899, 2011.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca |
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Origem |
Tipo/Formato |
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Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
30/11/1993 |
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Data da última atualização: |
13/05/2011 |
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Autoria: |
CALZAVARA, B. B. G. |
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Afiliação: |
BATISTA BENITO GABRIEL CALZAVARA, CPATU. |
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Título: |
Cajueiro. |
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Ano de publicação: |
1987 |
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Fonte/Imprenta: |
Belém, PA: EMBRAPA-CPATU, 1987. |
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Páginas: |
6 p. |
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Série: |
(EMBRAPA-CPATU. Recomendações básicas, 4). |
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Idioma: |
Português |
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Conteúdo: |
Clima e solo; Variedades; Ciclo vegetativo; Métodos de propagação; Produção de muda; Preparo da área; Espaçamento recomendado e concentração por área; Plantio; Tratos culturais; Floração e frutificação; Colheita; Produção; Consorciação. |
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Palavras-Chave: |
Brasil; Cajueiro; Cashew; Cultivation; Cultivo; Cultting; Fruit; Plantation; Production. |
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Thesagro: |
Anacardium Occidentale; Caju; Fruta; Muda; Plantio; Produção. |
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Thesaurus NAL: |
Amazonia. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/34607/1/RecBas-04.pdf
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Marc: |
LEADER 00989nam a2200325 a 4500
001 1383805
005 2011-05-13
008 1987 bl uuuu u0uu1 u #d
100 1 $aCALZAVARA, B. B. G.
245 $aCajueiro.
260 $aBelém, PA: EMBRAPA-CPATU$c1987
300 $a6 p.
490 $a(EMBRAPA-CPATU. Recomendações básicas, 4).
520 $aClima e solo; Variedades; Ciclo vegetativo; Métodos de propagação; Produção de muda; Preparo da área; Espaçamento recomendado e concentração por área; Plantio; Tratos culturais; Floração e frutificação; Colheita; Produção; Consorciação.
650 $aAmazonia
650 $aAnacardium Occidentale
650 $aCaju
650 $aFruta
650 $aMuda
650 $aPlantio
650 $aProdução
653 $aBrasil
653 $aCajueiro
653 $aCashew
653 $aCultivation
653 $aCultivo
653 $aCultting
653 $aFruit
653 $aPlantation
653 $aProduction
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