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Registro Completo |
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Biblioteca(s): |
Embrapa Café; Embrapa Unidades Centrais. |
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Data corrente: |
01/03/2011 |
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Data da última atualização: |
04/03/2011 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
DIOLA, V.; BRITO, G. G. G.; GALLINA, A. P.; CAIXETA, E. T.; SAKYAMA, N. S.; LOUREIRO, M. E. |
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Afiliação: |
UFV; UFV; BIOAGRO/UFV; EVELINE TEIXEIRA CAIXETA, SAPC; BIOAGRO/UFV; UFV/BIOAGRO. |
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Título: |
High density genetic mapping of resistance gene to race II of Hemileia vastatrix in híbrido de timor genotype. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas. |
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Idioma: |
Inglês |
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Conteúdo: |
Coffee leaf rust caused by the fungus Hemileia vastatrix is the most important disease of Coffea Arabica, and can be responsible for reductions about 35 to 50% in coffee production in infected fields. In the Híbrido de Timor (HT) populations were detected resistance genes, that alone or in association, could confer resistance to several different physiologic races of H. vastatrix. The objective of this study was to generate a high density genetic map surrounding a single rust resistance gene. The mapping population (F2 UFV 421-4) was composed by 224 plants, derived from F1 self-fertilized individuals, which were derived from the cross between HT UFV 427-15 and the susceptible clone Catuai Amarelo IAC 30 (UFV 2143-236). Phenotypic characterization was performed by infection rate analysis of leaf disc inoculated with monospore cell culture rust race IIb spores. Phenotipic segregation in this population follow a rate of 3 resistant to 1 susceptible individual. Two BSAs (Bulked Segregant Analysis), one for resistant and other for susceptible plants, were composed by 5 genotypes each. About 770 primer combinations were used in AFLP analysis, and 15 markers were obtained. This marker delimited a chromosomal region of 90 cM. The nearest marker was positioned at 3.2 cM of the resistance gene, and it is inside a region of 6.6 cM between the markers limiting both gene sides. This make possible the obtaining of the physical map and to facility the studies of the signaling the defense answer in the interaction coffee x H. vastarix. MenosCoffee leaf rust caused by the fungus Hemileia vastatrix is the most important disease of Coffea Arabica, and can be responsible for reductions about 35 to 50% in coffee production in infected fields. In the Híbrido de Timor (HT) populations were detected resistance genes, that alone or in association, could confer resistance to several different physiologic races of H. vastatrix. The objective of this study was to generate a high density genetic map surrounding a single rust resistance gene. The mapping population (F2 UFV 421-4) was composed by 224 plants, derived from F1 self-fertilized individuals, which were derived from the cross between HT UFV 427-15 and the susceptible clone Catuai Amarelo IAC 30 (UFV 2143-236). Phenotypic characterization was performed by infection rate analysis of leaf disc inoculated with monospore cell culture rust race IIb spores. Phenotipic segregation in this population follow a rate of 3 resistant to 1 susceptible individual. Two BSAs (Bulked Segregant Analysis), one for resistant and other for susceptible plants, were composed by 5 genotypes each. About 770 primer combinations were used in AFLP analysis, and 15 markers were obtained. This marker delimited a chromosomal region of 90 cM. The nearest marker was positioned at 3.2 cM of the resistance gene, and it is inside a region of 6.6 cM between the markers limiting both gene sides. This make possible the obtaining of the physical map and to facility the studies of the signaling the defense a... Mostrar Tudo |
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Thesagro: |
Coffea Arábica; Hemileia Vastatrix. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02177nam a2200193 a 4500
001 1880082
005 2011-03-04
008 2008 bl uuuu u00u1 u #d
100 1 $aDIOLA, V.
245 $aHigh density genetic mapping of resistance gene to race II of Hemileia vastatrix in híbrido de timor genotype.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas.$c2008
520 $aCoffee leaf rust caused by the fungus Hemileia vastatrix is the most important disease of Coffea Arabica, and can be responsible for reductions about 35 to 50% in coffee production in infected fields. In the Híbrido de Timor (HT) populations were detected resistance genes, that alone or in association, could confer resistance to several different physiologic races of H. vastatrix. The objective of this study was to generate a high density genetic map surrounding a single rust resistance gene. The mapping population (F2 UFV 421-4) was composed by 224 plants, derived from F1 self-fertilized individuals, which were derived from the cross between HT UFV 427-15 and the susceptible clone Catuai Amarelo IAC 30 (UFV 2143-236). Phenotypic characterization was performed by infection rate analysis of leaf disc inoculated with monospore cell culture rust race IIb spores. Phenotipic segregation in this population follow a rate of 3 resistant to 1 susceptible individual. Two BSAs (Bulked Segregant Analysis), one for resistant and other for susceptible plants, were composed by 5 genotypes each. About 770 primer combinations were used in AFLP analysis, and 15 markers were obtained. This marker delimited a chromosomal region of 90 cM. The nearest marker was positioned at 3.2 cM of the resistance gene, and it is inside a region of 6.6 cM between the markers limiting both gene sides. This make possible the obtaining of the physical map and to facility the studies of the signaling the defense answer in the interaction coffee x H. vastarix.
650 $aCoffea Arábica
650 $aHemileia Vastatrix
700 1 $aBRITO, G. G. G.
700 1 $aGALLINA, A. P.
700 1 $aCAIXETA, E. T.
700 1 $aSAKYAMA, N. S.
700 1 $aLOUREIRO, M. E.
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Embrapa Café (CNPCa) |
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| 4. |  | FALESI, I. C. (coord.). Levantamento detalhado dos solos do IPEAAOc. Manaus: MA-DNPEA-IPEAAOc, 1972. 63 p. (Boletim técnico, n. 1). Autores: Tarciso Ewerton Rodrigues, Raimundo Sousa dos Reis, Ivo Katuji Morikawa, Italo Caludio Falesi, Benedito Nelson R. da Silva(fotointepretação), Geraldo de Assis Guimarães, Edna de Carvalho Lopes, Joaquim Braga Bastos.| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental. |
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| 18. | | FALESI, I. C. Solos na área de influência de Carajás. In: SEMINÁRIO SOBRE DESENVOLVIMENTO ECONÔMICO E IMPACTO AMBIENTAL EM ÁREAS DE TRÓPICO ÚMIDO BRASILEIRO: A EXPERIÊNCIA DA CVRD, 1., 1986, Belém, PA. Anais... Rio de Janeiro: CVRD, 1987. p. 7-34.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 20. | | FALESI, I. C. Solos de gleba Carajás II (Setores V, VI, VII). In: SIMPÓSIO DO TRÓPICO ÚMIDO, 1., 1984, Belém, PA. Resumos. Belém, PA: EMBRAPA-CPATU, 1984. p. 30-31. (EMBRAPA-CPATU. Documentos, 31).| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 90 | |
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