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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/01/2011 |
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Data da última atualização: |
19/01/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, M. F. e; MIROLI, D. M. B. P.; CARDINALI, M. C. B.; MASCARENHAS, Y. P.; MARTINS, P. K.; FREITAS ASTUA, J. de; MACHADO, M. A. |
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Afiliação: |
Marina França e Silva, CNPDIA; Débora Marcondes Bastos Pereira Miroli, CNPDIA; Marcelo C. B. Cardinali, CNPDIA; Yvonne P. Mascarenhas, USP; Polyanna kelly Martins, APTA; JULIANA DE FREITAS ASTUA, CNPMF; Marcos Antonio Machado, APTA. |
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Título: |
Application of fourier transform infrared spectroscopy (FTIR) for early diagnosis of citrus greening disease. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. |
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Idioma: |
Inglês |
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Notas: |
120_PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010 |
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Conteúdo: |
Several diseases have caused great damage to citrus production worldwide. Among them, Greening is the most feared for its aggressiveness, speed of spread, and a lack of resistant variety. Infected plants goes through a long asymptomatic period (around 6 to 36 months), allowing the permanence of inocullum in the field. This study purpose to evaluate the capability of early diagnosis of spectroscopy at infrared with transformed Fourier (FTIR) for the Greening disease. For both, 60 citrus seedlings were inoculated with Candidatus Liberibater asiaticus (CLas) and 60 healthy seedlings (control) were kept in a greenhouse, which grown under controlled conditions of soil, irrigation and nutrients. Their leaves were measured monthly using FTIR spectroscopy during eight months. The evolution of the bacteria on inoculated plants was monitored by RT-qPCR (real-time quantitative PCR) amplification of CLas sequences. The spectra were analyzed using Principal Component Analysis (PCA). For all the months, it was shown separation between the asymptomatic and healthy leaves, emphasizing that significant chemical changes are occurring before symptomatic phase. Preliminary analyses showed that infected plants suffered a decreasing in concentrations of carbohydrate and chlorophyll. The results also showed that is possible to recognize a typical signature of sick leaf using FTIR spectroscopy, even in symptomatic phase, which must be studied as a feasible tool to develop a methodology for early citrus greening detection. MenosSeveral diseases have caused great damage to citrus production worldwide. Among them, Greening is the most feared for its aggressiveness, speed of spread, and a lack of resistant variety. Infected plants goes through a long asymptomatic period (around 6 to 36 months), allowing the permanence of inocullum in the field. This study purpose to evaluate the capability of early diagnosis of spectroscopy at infrared with transformed Fourier (FTIR) for the Greening disease. For both, 60 citrus seedlings were inoculated with Candidatus Liberibater asiaticus (CLas) and 60 healthy seedlings (control) were kept in a greenhouse, which grown under controlled conditions of soil, irrigation and nutrients. Their leaves were measured monthly using FTIR spectroscopy during eight months. The evolution of the bacteria on inoculated plants was monitored by RT-qPCR (real-time quantitative PCR) amplification of CLas sequences. The spectra were analyzed using Principal Component Analysis (PCA). For all the months, it was shown separation between the asymptomatic and healthy leaves, emphasizing that significant chemical changes are occurring before symptomatic phase. Preliminary analyses showed that infected plants suffered a decreasing in concentrations of carbohydrate and chlorophyll. The results also showed that is possible to recognize a typical signature of sick leaf using FTIR spectroscopy, even in symptomatic phase, which must be studied as a feasible tool to develop a methodology for early ci... Mostrar Tudo |
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Palavras-Chave: |
Candidatus Liberibater asiaticus; Plant disease. |
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Thesagro: |
Bactéria; Doença de Planta; Fruta Cítrica; Greening. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02492nam a2200265 a 4500
001 1872725
005 2011-01-19
008 2010 bl uuuu u00u1 u #d
100 1 $aSILVA, M. F. e
245 $aApplication of fourier transform infrared spectroscopy (FTIR) for early diagnosis of citrus greening disease.
260 $aIn: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM.$c2010
500 $a120_PS2 Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010
520 $aSeveral diseases have caused great damage to citrus production worldwide. Among them, Greening is the most feared for its aggressiveness, speed of spread, and a lack of resistant variety. Infected plants goes through a long asymptomatic period (around 6 to 36 months), allowing the permanence of inocullum in the field. This study purpose to evaluate the capability of early diagnosis of spectroscopy at infrared with transformed Fourier (FTIR) for the Greening disease. For both, 60 citrus seedlings were inoculated with Candidatus Liberibater asiaticus (CLas) and 60 healthy seedlings (control) were kept in a greenhouse, which grown under controlled conditions of soil, irrigation and nutrients. Their leaves were measured monthly using FTIR spectroscopy during eight months. The evolution of the bacteria on inoculated plants was monitored by RT-qPCR (real-time quantitative PCR) amplification of CLas sequences. The spectra were analyzed using Principal Component Analysis (PCA). For all the months, it was shown separation between the asymptomatic and healthy leaves, emphasizing that significant chemical changes are occurring before symptomatic phase. Preliminary analyses showed that infected plants suffered a decreasing in concentrations of carbohydrate and chlorophyll. The results also showed that is possible to recognize a typical signature of sick leaf using FTIR spectroscopy, even in symptomatic phase, which must be studied as a feasible tool to develop a methodology for early citrus greening detection.
650 $aBactéria
650 $aDoença de Planta
650 $aFruta Cítrica
650 $aGreening
653 $aCandidatus Liberibater asiaticus
653 $aPlant disease
700 1 $aMIROLI, D. M. B. P.
700 1 $aCARDINALI, M. C. B.
700 1 $aMASCARENHAS, Y. P.
700 1 $aMARTINS, P. K.
700 1 $aFREITAS ASTUA, J. de
700 1 $aMACHADO, M. A.
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