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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
20/12/2010 |
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Data da última atualização: |
19/01/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
LOCALI-FABRIS, E. C.; MAFRA, V. S.; MARTINS, P. K.; RIBEIRO- ALVES, M.; FRANCISCO, C. S.; FREITAS ASTUA, J. de; KISHI, L. T.; MACHADO, M. A. |
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Afiliação: |
Eliane Cristina Locali-Fabris, APTA; Valéria S. Mafra, APTA; Polyana Kelly. Martins, APTA; Marcelo Ribeiro-Alves, Fiocruz; Carolina S. Francisco, APTA; JULIANA DE FREITAS ASTUA, CNPMF; Luciano T. Kishi, APTA; Marcelo A. Machado, APTA. |
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Título: |
Gene set enrichment analysis in sweet orange plants infected by Candidatus Liberibacter americanus. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. |
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Idioma: |
Inglês |
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Notas: |
0058 PSO Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010 |
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Conteúdo: |
Huanglongbing (HLB) is known as the most devastating disease of citrus. Two species of Candidatus Liberibacter have been reported in Brazil, Ca. L. asiaticus (CaLas) and Ca. L. americanus (CaLam). Since CaLas is prevalent worldwide, most of the studies have focused that species and very little information is available on CaLam. With the objective to investigate differences in metabolic pathways of Citrus sinensis in response to CaLam, we used a genome- wide microarray approach to identify differentially expressed genes in symptomatic plants when compared to control plants. Hybridizations were performed by Roche Nimblegen with a customized 385K chip containing 32,000 unigenes of C. sinensis. The raw data were pre-processed using the Robust Multiarray Average (RMA). Normalized data were imported to R/Bioconductor where statistical analyses were performed. A Bayesian moderated t-test was calculated and p-values were adjusted for multiple comparisons by the false discovery rate correction. A total of 634 genes with P-values ?0.05, fold change |FC| ? 2.0 and odds probability ? 0.95 were considered differentially expressed. From those, 419 were up- and 215 down-regulated in symptomatic plants. In order to identify enriched gene ontologies regulated in infected plants we applied a gene set enrichment analysis approach and found 45 different biological processes including those involved in response to jasmonic acid stimulus, signaling and metabolism, 17 molecular functions, including oxygen binding, monooxygenase activity and oxidoreductase activity, 6 cellular components, mostly related to chloroplast, enriched at a statistically significant level of 0.001. MenosHuanglongbing (HLB) is known as the most devastating disease of citrus. Two species of Candidatus Liberibacter have been reported in Brazil, Ca. L. asiaticus (CaLas) and Ca. L. americanus (CaLam). Since CaLas is prevalent worldwide, most of the studies have focused that species and very little information is available on CaLam. With the objective to investigate differences in metabolic pathways of Citrus sinensis in response to CaLam, we used a genome- wide microarray approach to identify differentially expressed genes in symptomatic plants when compared to control plants. Hybridizations were performed by Roche Nimblegen with a customized 385K chip containing 32,000 unigenes of C. sinensis. The raw data were pre-processed using the Robust Multiarray Average (RMA). Normalized data were imported to R/Bioconductor where statistical analyses were performed. A Bayesian moderated t-test was calculated and p-values were adjusted for multiple comparisons by the false discovery rate correction. A total of 634 genes with P-values ?0.05, fold change |FC| ? 2.0 and odds probability ? 0.95 were considered differentially expressed. From those, 419 were up- and 215 down-regulated in symptomatic plants. In order to identify enriched gene ontologies regulated in infected plants we applied a gene set enrichment analysis approach and found 45 different biological processes including those involved in response to jasmonic acid stimulus, signaling and metabolism, 17 molecular functions, includin... Mostrar Tudo |
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Palavras-Chave: |
Huanglongbing; Plant disease. |
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Thesagro: |
Doença de Planta; Fruta Cítrica. |
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Thesaurus Nal: |
Candidatus Liberibacter; Citrus. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02656nam a2200277 a 4500
001 1870461
005 2011-01-19
008 2010 bl uuuu u00u1 u #d
100 1 $aLOCALI-FABRIS, E. C.
245 $aGene set enrichment analysis in sweet orange plants infected by Candidatus Liberibacter americanus.
260 $aIn: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM.$c2010
500 $a0058 PSO Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010
520 $aHuanglongbing (HLB) is known as the most devastating disease of citrus. Two species of Candidatus Liberibacter have been reported in Brazil, Ca. L. asiaticus (CaLas) and Ca. L. americanus (CaLam). Since CaLas is prevalent worldwide, most of the studies have focused that species and very little information is available on CaLam. With the objective to investigate differences in metabolic pathways of Citrus sinensis in response to CaLam, we used a genome- wide microarray approach to identify differentially expressed genes in symptomatic plants when compared to control plants. Hybridizations were performed by Roche Nimblegen with a customized 385K chip containing 32,000 unigenes of C. sinensis. The raw data were pre-processed using the Robust Multiarray Average (RMA). Normalized data were imported to R/Bioconductor where statistical analyses were performed. A Bayesian moderated t-test was calculated and p-values were adjusted for multiple comparisons by the false discovery rate correction. A total of 634 genes with P-values ?0.05, fold change |FC| ? 2.0 and odds probability ? 0.95 were considered differentially expressed. From those, 419 were up- and 215 down-regulated in symptomatic plants. In order to identify enriched gene ontologies regulated in infected plants we applied a gene set enrichment analysis approach and found 45 different biological processes including those involved in response to jasmonic acid stimulus, signaling and metabolism, 17 molecular functions, including oxygen binding, monooxygenase activity and oxidoreductase activity, 6 cellular components, mostly related to chloroplast, enriched at a statistically significant level of 0.001.
650 $aCandidatus Liberibacter
650 $aCitrus
650 $aDoença de Planta
650 $aFruta Cítrica
653 $aHuanglongbing
653 $aPlant disease
700 1 $aMAFRA, V. S.
700 1 $aMARTINS, P. K.
700 1 $aRIBEIRO- ALVES, M.
700 1 $aFRANCISCO, C. S.
700 1 $aFREITAS ASTUA, J. de
700 1 $aKISHI, L. T.
700 1 $aMACHADO, M. A.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
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| Registros recuperados : 9 | |
| 1. |  | CADORE, G. C.; CAMILLO, G.; SANGIONI, L. A.; VOGEL, F. S. F. Virulência e multiplicação de isolados de Toxoplasma gondii da região central do Rio Grande do Sul. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 6, p. 1026-1029, junho 2018 Título em inglês: Virulence and multiplication of Toxoplasma gondii isolates from the central region of Rio Grande do Sul, Brazil.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 2. | | PORTELLA, L. P.; CADORE, G. C.; LIMA, M. de; SANGIONI, L. A.; FISCHER, G.; VOGEL, F. S. F. Antibodies against Neospora caninum, Sarcocystis spp. and Toxoplasma gondii detected in buffaloes from Rio Grande do Sul, Brazil. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 10, p. 947-950, out. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 3. | | OLIVEIRA, C. S.; BRAUNIG, P.; KRAWCZAK, F.; LABRUNA, M. B.; BOTTON, S. A.; VOGEL, F. S. F.; SANGIONI, L. A. Detecção de proteínas imunorreativas de Rickettsia sp. cepa Mata Atlântica. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 1, p. 52-57, jan. 2017.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 4. | | ALVES, M. E. M.; CADORE, G. C.; OLIVEIRA, C. S.; PORTELLA, L. P.; SANGIONI, L. A.; VOGEL, F. S. F. Caracterização molecular de Sarcocystis spp. em amostras de carne. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 3, p. 425-429, março 2018 Título em inglês: Molecular characterization of Sarcocystis spp. in samples of meat.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 5. | | VASCONCELOS, J. S. P.; FERNANDES, F.; CADORE, G. C.; RATZLAFF, F. R.; FELIPETTO, L. G.; VOGEL, F. S. F.; BOTTON, S. A.; SANGIONI, L. A. Atividade acaricida de diferentes solventes sobre fêmeas ingurgitadas e larvas de Rhipicephalus microplus. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 6, p. 1125-1129, junho 2018 Título em inglês: Acaricidal activity of different solvents on engorged females and larvae of Rhipicephalus microplus.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 6. | | RAMOS, F.; PORTELLA, L. P.; RODRIGUES, F. de S.; REGINATO, C. Z.; CEZAR, A. S.; SANGIONI, L. A.; VOGEL, F. S. F. Anthelminthic resistance of gastrointestinal nematodes in sheep to monepantel treatment in central region of Rio Grande do Sul, Brazil. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 1, p. 48-52, janeiro 2018. Título em português: Resistência anti-helmíntica de nematódeos gastrointestinais de ovinos ao monepantel na região central do Rio Grande do Sul.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 7. | | RECH, D. V.; POTTER, L.; VAZ, C. S. L.; PEREIRA, D. I. B.; SANGIONI, L. A.; VARGAS, A. C.; BOTTON, S. de A. Antimicrobial resistance in nontyphoidal Salmonella isolated from and poultry-related samples in Brazil: 20-year meta-analysis. Foodborne Pathogens and Disease, v. 14, n. 2, p. 116-124, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 8. | | GRESSLER, L. T.; VARGAS, A. C. de; COSTA, M. M. da; SUTILI, F. J.; SCHWAB, M.; PEREIRA, D. I. B.; SANGIONI, L. A.; BOTTON, S. de A. Biofilm formation by Rhodococcus equi and putative association with macrolide resistance. Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 10, p. 835-841, out. 2015.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 9. | | CAMILLO, G.; MACHADO, M. E. A.; WEBER, A.; CADORE, G. C.; MENEZES, F. R.; PARDINI, L.; SANGIONI, L. A.; VOGEL, F. S. F. Prevalência de anticorpos e fatores de risco associados à infecção por Toxoplasma gondii em galinhas domésticas da zona rural de Santa Maria, Rio Grande do Sul. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 7, p. 1351-1357, julho 2018 Título em inglês: Prevalence of antibodies and risk factors associated with infection by Toxoplasma gondii in free range chickens of rural area of Santa Maria, Rio Grande do Sul, Brazil.| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 9 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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