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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
20/12/2010 |
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Data da última atualização: |
19/01/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
LOCALI-FABRIS, E. C.; MAFRA, V. S.; MARTINS, P. K.; RIBEIRO- ALVES, M.; FRANCISCO, C. S.; FREITAS ASTUA, J. de; KISHI, L. T.; MACHADO, M. A. |
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Afiliação: |
Eliane Cristina Locali-Fabris, APTA; Valéria S. Mafra, APTA; Polyana Kelly. Martins, APTA; Marcelo Ribeiro-Alves, Fiocruz; Carolina S. Francisco, APTA; JULIANA DE FREITAS ASTUA, CNPMF; Luciano T. Kishi, APTA; Marcelo A. Machado, APTA. |
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Título: |
Gene set enrichment analysis in sweet orange plants infected by Candidatus Liberibacter americanus. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. |
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Idioma: |
Inglês |
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Notas: |
0058 PSO Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010 |
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Conteúdo: |
Huanglongbing (HLB) is known as the most devastating disease of citrus. Two species of Candidatus Liberibacter have been reported in Brazil, Ca. L. asiaticus (CaLas) and Ca. L. americanus (CaLam). Since CaLas is prevalent worldwide, most of the studies have focused that species and very little information is available on CaLam. With the objective to investigate differences in metabolic pathways of Citrus sinensis in response to CaLam, we used a genome- wide microarray approach to identify differentially expressed genes in symptomatic plants when compared to control plants. Hybridizations were performed by Roche Nimblegen with a customized 385K chip containing 32,000 unigenes of C. sinensis. The raw data were pre-processed using the Robust Multiarray Average (RMA). Normalized data were imported to R/Bioconductor where statistical analyses were performed. A Bayesian moderated t-test was calculated and p-values were adjusted for multiple comparisons by the false discovery rate correction. A total of 634 genes with P-values ?0.05, fold change |FC| ? 2.0 and odds probability ? 0.95 were considered differentially expressed. From those, 419 were up- and 215 down-regulated in symptomatic plants. In order to identify enriched gene ontologies regulated in infected plants we applied a gene set enrichment analysis approach and found 45 different biological processes including those involved in response to jasmonic acid stimulus, signaling and metabolism, 17 molecular functions, including oxygen binding, monooxygenase activity and oxidoreductase activity, 6 cellular components, mostly related to chloroplast, enriched at a statistically significant level of 0.001. MenosHuanglongbing (HLB) is known as the most devastating disease of citrus. Two species of Candidatus Liberibacter have been reported in Brazil, Ca. L. asiaticus (CaLas) and Ca. L. americanus (CaLam). Since CaLas is prevalent worldwide, most of the studies have focused that species and very little information is available on CaLam. With the objective to investigate differences in metabolic pathways of Citrus sinensis in response to CaLam, we used a genome- wide microarray approach to identify differentially expressed genes in symptomatic plants when compared to control plants. Hybridizations were performed by Roche Nimblegen with a customized 385K chip containing 32,000 unigenes of C. sinensis. The raw data were pre-processed using the Robust Multiarray Average (RMA). Normalized data were imported to R/Bioconductor where statistical analyses were performed. A Bayesian moderated t-test was calculated and p-values were adjusted for multiple comparisons by the false discovery rate correction. A total of 634 genes with P-values ?0.05, fold change |FC| ? 2.0 and odds probability ? 0.95 were considered differentially expressed. From those, 419 were up- and 215 down-regulated in symptomatic plants. In order to identify enriched gene ontologies regulated in infected plants we applied a gene set enrichment analysis approach and found 45 different biological processes including those involved in response to jasmonic acid stimulus, signaling and metabolism, 17 molecular functions, includin... Mostrar Tudo |
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Palavras-Chave: |
Huanglongbing; Plant disease. |
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Thesagro: |
Doença de Planta; Fruta Cítrica. |
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Thesaurus Nal: |
Candidatus Liberibacter; Citrus. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02656nam a2200277 a 4500
001 1870461
005 2011-01-19
008 2010 bl uuuu u00u1 u #d
100 1 $aLOCALI-FABRIS, E. C.
245 $aGene set enrichment analysis in sweet orange plants infected by Candidatus Liberibacter americanus.
260 $aIn: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM.$c2010
500 $a0058 PSO Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010
520 $aHuanglongbing (HLB) is known as the most devastating disease of citrus. Two species of Candidatus Liberibacter have been reported in Brazil, Ca. L. asiaticus (CaLas) and Ca. L. americanus (CaLam). Since CaLas is prevalent worldwide, most of the studies have focused that species and very little information is available on CaLam. With the objective to investigate differences in metabolic pathways of Citrus sinensis in response to CaLam, we used a genome- wide microarray approach to identify differentially expressed genes in symptomatic plants when compared to control plants. Hybridizations were performed by Roche Nimblegen with a customized 385K chip containing 32,000 unigenes of C. sinensis. The raw data were pre-processed using the Robust Multiarray Average (RMA). Normalized data were imported to R/Bioconductor where statistical analyses were performed. A Bayesian moderated t-test was calculated and p-values were adjusted for multiple comparisons by the false discovery rate correction. A total of 634 genes with P-values ?0.05, fold change |FC| ? 2.0 and odds probability ? 0.95 were considered differentially expressed. From those, 419 were up- and 215 down-regulated in symptomatic plants. In order to identify enriched gene ontologies regulated in infected plants we applied a gene set enrichment analysis approach and found 45 different biological processes including those involved in response to jasmonic acid stimulus, signaling and metabolism, 17 molecular functions, including oxygen binding, monooxygenase activity and oxidoreductase activity, 6 cellular components, mostly related to chloroplast, enriched at a statistically significant level of 0.001.
650 $aCandidatus Liberibacter
650 $aCitrus
650 $aDoença de Planta
650 $aFruta Cítrica
653 $aHuanglongbing
653 $aPlant disease
700 1 $aMAFRA, V. S.
700 1 $aMARTINS, P. K.
700 1 $aRIBEIRO- ALVES, M.
700 1 $aFRANCISCO, C. S.
700 1 $aFREITAS ASTUA, J. de
700 1 $aKISHI, L. T.
700 1 $aMACHADO, M. A.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 124 | |
| 3. |  | FERANDES, M. F. R.; FRANCISCO, M. da S.; SOARES, L. H. de B.; ZILLI, J. E. Alternativa biológica para o tratamento de sementes contra fungos In: CONGRESSO BRASILEIRO DE SOJA, 9., 2022, Foz do iguaçu, PR. Desafios para a produtividade sustentável no Mercosul: resumos. Brasília, DF: Embrapa, 2022. 328 p. p. 305| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 11. | | URQUIAGA, S.; ALVES, B. J. R.; JANTALIA, C. P.; SOARES, L. H. B.; BODDEY, R. M. Mudanças ambientais: seqüestro de carbono, gases de efeito estufa e educação ambiental. In: REUNIÃO BRASILEIRA DE MANEJO E CONSERVAÇÃO DO SOLO E DA ÁGUA, 17., 2008, Rio de Janeiro. Manejo e conservação do solo e da água no contexto das mudanças ambientais. Rio de Janeiro: SBCS: Embrapa Solos: Embrapa Agrobiologia, 2008. 6 p. 1 CD-ROM. (Embrapa Solos. Documentos, 101).| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 13. | | URQUIAGA, S.; ALVES, B. J. R.; SOARES, L. H. B.; JANTALIA, C. P.; BODDEY, R. M. La fijación biológica de nitrógeno: suporte essensial para la agroenergia In: REUNIÓN CIENTIFICO TÉCNICA DE BIOLOGIA DEL SUELO Y FIJACIÓN BIOLÓGICA DE NITRÓGENIO- REBIOS, 7., 2009, San Miguel de Tucaumán, AR. Libro de resúmenes...San Miguel de Tucaumán: Universidad Nacional de Tucumán, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 16. | | BAHIA, P. P.; ARAUJO, J. L. S. de; SOARES, L. H. de B.; REIS, V. M. Desenvolvimento de bioprocesso para produção de inoculante de cana-de-açúcar através do co-cultivo de duas espécies de herbaspirillum In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., FERTBIO 2016. Goiânia. Rumo aos novos desafios: anais. Goiânia: UFG, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 17. | | SOARES, L. H. de B.; ALVES, B. J. R.; BODDEY, R. M.; URQUIAGA, S. Balanço energético da cultura da mandioca (Manihot esculenta Crantz) em um programa para produção de bioetanol. In: CONGRESSO BRASILEIRO DE AGRONOMIA, 25., 2007, Guarapari, ES. Agroenergia: o futuro do planeta em nossas mãos, resumo... Guarapari: CONFAEAB, 2007. 4 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 20. | | ALVES, B. J. R.; JANTALIA, C. P.; SOARES, L. H. B.; URQUIAGA, S.; BODDEY, R. M. Diversifying crop rotations with N2-fixing legumes. In: WORLD CONGRESS ON CONSERVATION AGRICULTURES, 4., 4 a 7 february 2009, New Delhi, India. Trabalhos... Nova Déli: Indian Council of Agricultural Research, 2009.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 124 | |
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