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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
30/09/2010 |
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Data da última atualização: |
30/06/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SUAREZ, W. T.; PESSOA NETO, O. D.; SANTOS, V. B. dos; NOGUEIRA, A. R. de A.; FARIA, R. C.; FATIBELLO FILHO, O.; PUYOL, M.; ALONSO, J. |
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Afiliação: |
WILLIAN TOITO SUAREZ, UFSCar/SÃO CARLOS, SP; OSMUNDO DANTAS PESSOA-NETO, UFSCar/SÃO CARLOS, SP; VAGNER BEZERRA DOS SANTOS, UFSCar/SÃO CARLOS, SP; ANA RITA DE ARAUJO NOGUEIRA, CPPSE; RONALDO CENSI FARIA, UFSCar/SÃO CARLOS, SP; ORLANDO FATIBELLO-FILHO, UFSCar/SÃO CARLOS, SP; MAR PUYOL, UNIVERSIT AUTONOMA DE BARCELONA; J. ALONSO, UNIVERSIT AUTONOMA DE BARCELONA. |
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Título: |
A compact miniaturized continuous flow system for the determination of urea content in milk. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Analytical & Bioanalytical Chemistry, n. 398, p. 1525-1533, 2010 |
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DOI: |
10.1007/s00216-010-4052-6 |
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Idioma: |
Português |
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Conteúdo: |
A multicommutation-based flow system with photometric detection was developed, employing an analytical microsystem constructed with low temperature cofired ceramics (LTCC) technology, a solid-phase reactor containing particles of Canavalia ensiformis DC (urease source) immobilized with glutaraldehyde, and a miniphotometer coupled directly to the microsystem which monolithically integrates a continuous flow cell. The determination of urea in milk was based on the hydrolysis of urea in the solid-phase reactor and the ammonium ions produced were monitored using the Berthelot reaction. The analytical curve was linear in the urea concentration range from 1.0×10 -4 to 5.0×10 -3 mol L -1 with a limit of detection of 8.0×10 -6 mol L -1. The relative standard deviation (RSD) for a 2.0×10 -3 mol L -1 urea solution was lower than 0.4% (n=10) and the sample throughput was 13 h?1. To check the reproducibility of the flow system, calibration curves were obtained with freshly prepared solutions on different days and the RSD obtained was 4.7% (n=6). Accuracy was assessed by comparing the results of the proposed method with those from the official procedure and the data are in close agreement, at a 95% confidence level. |
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Palavras-Chave: |
Berthelot’s reaction; Low temperature co-fired ceramics. |
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Thesaurus Nal: |
milk; urea. |
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Categoria do assunto: |
W Química e Física |
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Marc: |
LEADER 02032naa a2200265 a 4500
001 1863311
005 2023-06-30
008 2010 bl uuuu u00u1 u #d
024 7 $a10.1007/s00216-010-4052-6$2DOI
100 1 $aSUAREZ, W. T.
245 $aA compact miniaturized continuous flow system for the determination of urea content in milk.$h[electronic resource]
260 $c2010
520 $aA multicommutation-based flow system with photometric detection was developed, employing an analytical microsystem constructed with low temperature cofired ceramics (LTCC) technology, a solid-phase reactor containing particles of Canavalia ensiformis DC (urease source) immobilized with glutaraldehyde, and a miniphotometer coupled directly to the microsystem which monolithically integrates a continuous flow cell. The determination of urea in milk was based on the hydrolysis of urea in the solid-phase reactor and the ammonium ions produced were monitored using the Berthelot reaction. The analytical curve was linear in the urea concentration range from 1.0×10 -4 to 5.0×10 -3 mol L -1 with a limit of detection of 8.0×10 -6 mol L -1. The relative standard deviation (RSD) for a 2.0×10 -3 mol L -1 urea solution was lower than 0.4% (n=10) and the sample throughput was 13 h?1. To check the reproducibility of the flow system, calibration curves were obtained with freshly prepared solutions on different days and the RSD obtained was 4.7% (n=6). Accuracy was assessed by comparing the results of the proposed method with those from the official procedure and the data are in close agreement, at a 95% confidence level.
650 $amilk
650 $aurea
653 $aBerthelot’s reaction
653 $aLow temperature co-fired ceramics
700 1 $aPESSOA NETO, O. D.
700 1 $aSANTOS, V. B. dos
700 1 $aNOGUEIRA, A. R. de A.
700 1 $aFARIA, R. C.
700 1 $aFATIBELLO FILHO, O.
700 1 $aPUYOL, M.
700 1 $aALONSO, J.
773 $tAnalytical & Bioanalytical Chemistry$gn. 398, p. 1525-1533, 2010
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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