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 | Acesso ao texto completo restrito à biblioteca da Embrapa Rondônia. Para informações adicionais entre em contato com cpafro.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
08/07/2010 |
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Data da última atualização: |
05/08/2024 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
LAUREANO, M. M. M.; OTAVIANO, A. R.; LIMA, A. L. F.; COSTA, R. B.; SALMAN, A. K. D.; SENA, J. A. D.; TONHATI, H.; ALBUQUERQUE, L. G. de. |
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Afiliação: |
MONYKA MARIANNA MASSOLINI LAUREANO, UNIVERSIDADE ESTADUAL PAULISTA "JÚLIO DE MESQUITA FILHO"; ANTÔNIO ROBERTO OTAVIANO, UNIVERSIDADE ESTADUAL PAULISTA "JÚLIO DE MESQUITA FILHO"; ANDRÉ LUÍS FERREIRA LIMA, UNIVERSIDADE ESTADUAL PAULISTA "JÚLIO DE MESQUITA FILHO"; RAPHAEL BERMAL COSTA, UNIVERSIDADE ESTADUAL PAULISTA "JÚLIO DE MESQUITA FILHO"; ANA KARINA DIAS SALMAN, CPAF-RO; JANETE APPARECIDA DESIDÉRIO SENA, UNIVERSIDADE ESTADUAL PAULISTA "JÚLIO DE MESQUITA FILHO"; HUMBERTO TONHATI, UNIVERSIDADE ESTADUAL PAULISTA "JÚLIO DE MESQUITA FILHO"; LÚCIA GALVÃO DE ALBUQUERQUE, UNIVERSIDADE ESTADUAL PAULISTA "JÚLIO DE MESQUITA FILHO". |
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Título: |
Characterization and polymorphism screening of IGF-I and prolactin genes in Nelore heifers. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Italian Journal of Animal Science, Bologna, v. 8, n. 2, p. 277-283, 2009. |
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DOI: |
https://doi.org/10.4081/ijas.2009.277 |
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Idioma: |
Inglês |
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Conteúdo: |
Insulin growth factor I (IGF-I) and prolactin (PRL) are peptide hormones that exert complementary effects on reproductive traits by acting on folliculogenesis. In view of the lack of information about the IGF-I and PRL genes in Bos indicus, the objective of this study was to partially characterize the promoter regions of these genes and to screen animals of different ages at first pregnancy for the presence of polymorphisms in these regions. In addition, we determined whether polymorphisms influence the regulation of the two hormone genes, evaluating their association with sexual precocity. The animals were divided into three groups according to age at first pregnancy: 1) 100 heifers considered to be sexually precocious that became pregnant at 15-16 months of age, 2) 100 heifers that became pregnant during the normal breeding season at 24 months of age, and 3) 100 heifers that did not become pregnant until 24 months of age. For the IGF-I gene, PCR-RFLP-SnaBI analysis showed the presence of genotypes AB and BB at frequencies of 0.02 and 0.98, respectively. Sequencing of the IGF-I gene fragment revealed a single nitrogen base change from cytosine to thymine, corresponding to the restriction site of SnaBI. The polymorphisms identified in the 5’-flanking region of the IGF-I gene may serve as a basis for future studies of molecular markers in cattle. For the PRL gene, PCR-RFLP-HaeIII analysis showed the presence of only one migration pattern, a finding characterizing the region studied as monomorphic. The study of other regions in the IGF-I and PRL genes might provide molecular data that can be used in the future for the selection of sexually precocious animals. MenosInsulin growth factor I (IGF-I) and prolactin (PRL) are peptide hormones that exert complementary effects on reproductive traits by acting on folliculogenesis. In view of the lack of information about the IGF-I and PRL genes in Bos indicus, the objective of this study was to partially characterize the promoter regions of these genes and to screen animals of different ages at first pregnancy for the presence of polymorphisms in these regions. In addition, we determined whether polymorphisms influence the regulation of the two hormone genes, evaluating their association with sexual precocity. The animals were divided into three groups according to age at first pregnancy: 1) 100 heifers considered to be sexually precocious that became pregnant at 15-16 months of age, 2) 100 heifers that became pregnant during the normal breeding season at 24 months of age, and 3) 100 heifers that did not become pregnant until 24 months of age. For the IGF-I gene, PCR-RFLP-SnaBI analysis showed the presence of genotypes AB and BB at frequencies of 0.02 and 0.98, respectively. Sequencing of the IGF-I gene fragment revealed a single nitrogen base change from cytosine to thymine, corresponding to the restriction site of SnaBI. The polymorphisms identified in the 5’-flanking region of the IGF-I gene may serve as a basis for future studies of molecular markers in cattle. For the PRL gene, PCR-RFLP-HaeIII analysis showed the presence of only one migration pattern, a finding characterizing the region s... Mostrar Tudo |
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Palavras-Chave: |
Molecular marker; Precocidade sexual; RFLP; Sexual precocity. |
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Thesagro: |
Bovinocultura; Gado de Corte; Marcador Molecular. |
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Thesaurus Nal: |
beef cattle. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02629naa a2200313 a 4500
001 1857176
005 2024-08-05
008 2009 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4081/ijas.2009.277$2DOI
100 1 $aLAUREANO, M. M. M.
245 $aCharacterization and polymorphism screening of IGF-I and prolactin genes in Nelore heifers.$h[electronic resource]
260 $c2009
520 $aInsulin growth factor I (IGF-I) and prolactin (PRL) are peptide hormones that exert complementary effects on reproductive traits by acting on folliculogenesis. In view of the lack of information about the IGF-I and PRL genes in Bos indicus, the objective of this study was to partially characterize the promoter regions of these genes and to screen animals of different ages at first pregnancy for the presence of polymorphisms in these regions. In addition, we determined whether polymorphisms influence the regulation of the two hormone genes, evaluating their association with sexual precocity. The animals were divided into three groups according to age at first pregnancy: 1) 100 heifers considered to be sexually precocious that became pregnant at 15-16 months of age, 2) 100 heifers that became pregnant during the normal breeding season at 24 months of age, and 3) 100 heifers that did not become pregnant until 24 months of age. For the IGF-I gene, PCR-RFLP-SnaBI analysis showed the presence of genotypes AB and BB at frequencies of 0.02 and 0.98, respectively. Sequencing of the IGF-I gene fragment revealed a single nitrogen base change from cytosine to thymine, corresponding to the restriction site of SnaBI. The polymorphisms identified in the 5’-flanking region of the IGF-I gene may serve as a basis for future studies of molecular markers in cattle. For the PRL gene, PCR-RFLP-HaeIII analysis showed the presence of only one migration pattern, a finding characterizing the region studied as monomorphic. The study of other regions in the IGF-I and PRL genes might provide molecular data that can be used in the future for the selection of sexually precocious animals.
650 $abeef cattle
650 $aBovinocultura
650 $aGado de Corte
650 $aMarcador Molecular
653 $aMolecular marker
653 $aPrecocidade sexual
653 $aRFLP
653 $aSexual precocity
700 1 $aOTAVIANO, A. R.
700 1 $aLIMA, A. L. F.
700 1 $aCOSTA, R. B.
700 1 $aSALMAN, A. K. D.
700 1 $aSENA, J. A. D.
700 1 $aTONHATI, H.
700 1 $aALBUQUERQUE, L. G. de
773 $tItalian Journal of Animal Science, Bologna$gv. 8, n. 2, p. 277-283, 2009.
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Embrapa Rondônia (CPAF-RO) |
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| Registros recuperados : 2 | |
| 1. |  | NAVES, R. de L.; SANTANA, A. P. S.; PAPA, M. F. S.; SOUZA, R. T. de; CELOTO, M. I. B.; SANTOS, J. A. Atividade fungitóxica in vitro de extrato de folhas de pacari sobre uredioniósporos de Phakosora euvitis. Tropical Plant Pathology, Brasília, DF, v. 34, p. S45, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia; XLII Annual Meeting of the Brazilian Pthytopathologiacal Society, Rio de Janeiro, ago. 2009. Resumo 163.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 2. | | SANTANA, A. P. S.; PAPA, M. A. S.; NAVES, R. de L.; SOUZA, R. T. de; SANTOS, J. A.; CELOTO, M. I. B. Inibição da germinação in vitro de uredioniósporos de Phakosora euvitis por extrato de folhas de melão-de-sãocaetano em diferentes concentrações. Tropical Plant Pathology, Brasília, DF, v. 34, p. S45, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia; XLII Annual Meeting of the Brazilian Pthytopathologiacal Society, Rio de Janeiro, ago. 2009. Resumo 164.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| Registros recuperados : 2 | |
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