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Registro Completo |
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
10/01/2010 |
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Data da última atualização: |
22/02/2013 |
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Autoria: |
CHUNWONGSE, J.; MARTIN, G. B.; TANKSLEY, S. D. |
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Título: |
Pre-germination genotypic screening using PCR amplification of half-seeds. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Theoretical and Applied Genetics, v. 86, p. 694-698, 1993. |
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Idioma: |
Inglês |
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Conteúdo: |
A simple and rapid PCR-based method has been developed for determining the genotype of seeds before germination. Single half-seeds of rice (Oryza sativa L.) and wheat (Triticum aestivum L. em. Thell.) were preincubated, without grinding, in an aqueous extraction buffer. The resulting supernatants were then used in polymerase chain reaction (PCR) with oligonucleotide primers corresponding to rice single-copy sequences or a wheat microsatellite repeat. PCR products of identical size were amplified using either the half-seed extract or DNA isolated from leaf tissue. The remnant half-seeds can be maintained in ordered arrays using microtiter plates allowing the recovery of selected genotypes. Pre-germination genotypic screening of seed populations as described in this report should be useful for a variety of applications in plant breeding and genetics studies. |
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Palavras-Chave: |
extração de DNA; PCR; RAPD; RFLP; Seleção assistida por marcadores. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01434naa a2200205 a 4500
001 1840296
005 2013-02-22
008 1993 bl --- 0-- u #d
100 1 $aCHUNWONGSE, J.
245 $aPre-germination genotypic screening using PCR amplification of half-seeds.
260 $c1993
520 $aA simple and rapid PCR-based method has been developed for determining the genotype of seeds before germination. Single half-seeds of rice (Oryza sativa L.) and wheat (Triticum aestivum L. em. Thell.) were preincubated, without grinding, in an aqueous extraction buffer. The resulting supernatants were then used in polymerase chain reaction (PCR) with oligonucleotide primers corresponding to rice single-copy sequences or a wheat microsatellite repeat. PCR products of identical size were amplified using either the half-seed extract or DNA isolated from leaf tissue. The remnant half-seeds can be maintained in ordered arrays using microtiter plates allowing the recovery of selected genotypes. Pre-germination genotypic screening of seed populations as described in this report should be useful for a variety of applications in plant breeding and genetics studies.
653 $aextração de DNA
653 $aPCR
653 $aRAPD
653 $aRFLP
653 $aSeleção assistida por marcadores
700 1 $aMARTIN, G. B.
700 1 $aTANKSLEY, S. D.
773 $tTheoretical and Applied Genetics$gv. 86, p. 694-698, 1993.
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