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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
19/03/2010 |
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Data da última atualização: |
28/05/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, L. R.; MARCELINO, F. C.; BARCELLOS, F. G.; RODRIGUES, E. P.; MEGIAS, M.; HUNGRIA, M. |
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Afiliação: |
LUCIANA RUANO OLIVEIRA, UEL; FRANCISMAR CORREA MARCELINO, CNPSO; FERNANDO GOMES BARCELLOS; ELISETE PAINS RODRIGUES; MANUEL MEGIAS, UNIVERSIDAD DE SEVILHA; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
The nodC, nodG, and glgX genes of Rhizobium tropici strain PRF 81. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Functional & Integrative Genomics, v. 10, n. 3, p. 425-431, Aug. 2010. |
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DOI: |
10.1007/s10142-009-0151-x |
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Idioma: |
Inglês |
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Conteúdo: |
Rhizobium tropici is a diazotrophic microsymbiont of common bean (Phaseolus vulgaris L.) that encompasses important but still poorly studied tropical strains, and a recent significant contribution to the knowledge of the species was the publication of a genomic draft of strain PRF 81, which revealed several novel genes [Pinto et al. Funct Int Gen 9:263?270, 2009]. In this study, we investigated the transcription of nodC, nodG, and glgX genes, located in the nod operon of PRF 81 strain, by reverse-transcription quantitative PCR. All three genes showed low levels of transcription when the cells were grown until exponential growth phase in the presence of common-bean-seed exudates or of the root nod-gene inducer naringenin. However, when cells at the exponential phase of growth were incubated with seed exudates, transcription occurred after only 5 min, and nodC, nodG, and glgX were transcribed 121.97-, 14.86-, and 50.29-fold more than the control, respectively, followed by a rapid decrease in gene transcription. Much lower levels of transcription were observed in the presence of naringenin; furthermore, maximum transcription required 8 h of incubation for all three genes. In light of these results, the mechanisms of induction of the nodulation genes by flavonoids are discussed. |
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Palavras-Chave: |
Reverse transcription quantitative-PCR. |
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Thesagro: |
Feijao; Fixacao de nitrogenio; Gene; Phaseolus Vulgaris; Simbiose. |
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Thesaurus Nal: |
Beans; Genes; Nitrogen fixation; Rhizobium tropici; Symbiosis. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02216naa a2200325 a 4500
001 1661862
005 2025-05-28
008 2010 bl uuuu u00u1 u #d
024 7 $a10.1007/s10142-009-0151-x$2DOI
100 1 $aOLIVEIRA, L. R.
245 $aThe nodC, nodG, and glgX genes of Rhizobium tropici strain PRF 81.$h[electronic resource]
260 $c2010
520 $aRhizobium tropici is a diazotrophic microsymbiont of common bean (Phaseolus vulgaris L.) that encompasses important but still poorly studied tropical strains, and a recent significant contribution to the knowledge of the species was the publication of a genomic draft of strain PRF 81, which revealed several novel genes [Pinto et al. Funct Int Gen 9:263?270, 2009]. In this study, we investigated the transcription of nodC, nodG, and glgX genes, located in the nod operon of PRF 81 strain, by reverse-transcription quantitative PCR. All three genes showed low levels of transcription when the cells were grown until exponential growth phase in the presence of common-bean-seed exudates or of the root nod-gene inducer naringenin. However, when cells at the exponential phase of growth were incubated with seed exudates, transcription occurred after only 5 min, and nodC, nodG, and glgX were transcribed 121.97-, 14.86-, and 50.29-fold more than the control, respectively, followed by a rapid decrease in gene transcription. Much lower levels of transcription were observed in the presence of naringenin; furthermore, maximum transcription required 8 h of incubation for all three genes. In light of these results, the mechanisms of induction of the nodulation genes by flavonoids are discussed.
650 $aBeans
650 $aGenes
650 $aNitrogen fixation
650 $aRhizobium tropici
650 $aSymbiosis
650 $aFeijao
650 $aFixacao de nitrogenio
650 $aGene
650 $aPhaseolus Vulgaris
650 $aSimbiose
653 $aReverse transcription quantitative-PCR
700 1 $aMARCELINO, F. C.
700 1 $aBARCELLOS, F. G.
700 1 $aRODRIGUES, E. P.
700 1 $aMEGIAS, M.
700 1 $aHUNGRIA, M.
773 $tFunctional & Integrative Genomics$gv. 10, n. 3, p. 425-431, Aug. 2010.
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Embrapa Soja (CNPSO) |
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| 1. |  | SILVA, L. A. R.; OFICO, A. V.; BATISTA, N. R.; SOUZA, K. M. R. de; FREITAS, H. B. de; JULIANO, R. S.; KIEFER, C. Associação de diferentes níveis de mandioca, moringa e bocaiuva sobre a qualidade interna de ovos brancos tipo caipira. MOSTRA CIENTÍFICA FAMEZ, 8., 2015, Campo Grande. Anais... Campo Grande, MS: UFMS, 2015. p. 72-74| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 2. | | BARTZ, I.; OFICO, A. V.; BATISTA, N. R.; SOUZA, K. M. R. de; KIEFER, C.; JULIANO, R. S.; BERNO, P. R. Qualidade externa de ovos brancos do tipo caipira: associação de níveis de mandioca, moringa e bocaiúva na alimentação de poedeiras. MOSTRA CIENTÍFICA FAMEZ, 8., 2015, Campo Grande. Anais... Campo Grande, MS: UFMS, 2015. p. 112-114| Tipo: Resumo em Anais de Congresso |
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| 3. | | MACIE, V. A.; NASCIMENTO, K. R. de S.; KIEFER, C.; JULIANO, R. S.; LISITA, F. O.; SILVA, T. R. da; COPAT, L. L. P.; CHAVES, N. R. B.; FREITAS, H. B. de; ROSA, M. S. R. Metabolizable energy and metabolizability coefficients of moringa and bocaiuva for slow-growing broilers at different ages. Journal of Agricultural Studies, v. 8, n. 3, p. 207-220, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Pantanal. |
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| 4. | | OFIÇO, A. V.; NASCIMENTO, K. M. R. de S.; KIEFER, C.; JULIANO, R. S.; LISITA, F. O.; FREITAS, H. B. de; SILVA, T. R. da; COPAT, L. L. P.; CHAVES, N. R. B.; SILVA, L. A. R.; LEITE, J. V.; SANTANA, P. G.; OLIVEIRA, N. G. de. Egg quality of laying hens fed with cassava (Manihot esculenta), moringa (Moringa oleifera) and bocaiuva (Acrocomia aculeata) in semi-intensive rearing system. Research, Society and Development, v. 10, n. 6, e1541064828, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Pantanal. |
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