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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
19/03/2010 |
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Data da última atualização: |
19/01/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, L. R.; MARCELINO, F. C.; BARCELLOS, F. G.; RODRIGUES, E. P.; MEGIAS, M.; HUNGRIA, M. |
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Afiliação: |
LUCIANA RUANO OLIVEIRA, UEL - CNPq; FRANCISMAR CORREA MARCELINO, CNPSo; FERNANDO GOMES BARCELLOS, CNPSo; ELISETE PAINS RODRIGUES, CNPSo - CNPq; MANUEL MEGIAS, Universidad de Sevilha; MARIANGELA HUNGRIA DA CUNHA, CNPSo. |
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Título: |
The nodC, nodG, and glgX genes of Rhizobium tropici strain PRF 81. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Functional & Integrative Genomics, Heidelberg, v. 10, n. 3, p. 425-431, Aug. 2010. |
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DOI: |
DOI 10.1007/s10142-009-0151-x |
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Idioma: |
Inglês |
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Conteúdo: |
Rhizobium tropici is a diazotrophic microsymbiont of common bean (Phaseolus vulgaris L.) that encompasses important but still poorly studied tropical strains, and a recent significant contribution to the knowledge of the species was the publication of a genomic draft of strain PRF 81, which revealed several novel genes [Pinto et al. Funct Int Gen 9:263?270, 2009]. In this study, we investigated the transcription of nodC, nodG, and glgX genes, located in the nod operon of PRF 81 strain, by reverse-transcription quantitative PCR. All three genes showed low levels of transcription when the cells were grown until exponential growth phase in the presence of common-bean-seed exudates or of the root nod-gene inducer naringenin. However, when cells at the exponential phase of growth were incubated with seed exudates, transcription occurred after only 5 min, and nodC, nodG, and glgX were transcribed 121.97-, 14.86-, and 50.29-fold more than the control, respectively, followed by a rapid decrease in gene transcription. Much lower levels of transcription were observed in the presence of naringenin; furthermore, maximum transcription required 8 h of incubation for all three genes. In light of these results, the mechanisms of induction of the nodulation genes by flavonoids are discussed. |
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Palavras-Chave: |
Reverse transcription quantitative-PCR. |
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Thesagro: |
Feijao; Fixacao de nitrogenio; Gene; Phaseolus Vulgaris; Simbiose. |
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Thesaurus Nal: |
Beans; Genes; Nitrogen fixation; Rhizobium tropici; Symbiosis. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02209naa a2200325 a 4500
001 1661862
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008 2010 bl uuuu u00u1 u #d
024 7 $aDOI 10.1007/s10142-009-0151-x$2DOI
100 1 $aOLIVEIRA, L. R.
245 $aThe nodC, nodG, and glgX genes of Rhizobium tropici strain PRF 81.
260 $c2010
520 $aRhizobium tropici is a diazotrophic microsymbiont of common bean (Phaseolus vulgaris L.) that encompasses important but still poorly studied tropical strains, and a recent significant contribution to the knowledge of the species was the publication of a genomic draft of strain PRF 81, which revealed several novel genes [Pinto et al. Funct Int Gen 9:263?270, 2009]. In this study, we investigated the transcription of nodC, nodG, and glgX genes, located in the nod operon of PRF 81 strain, by reverse-transcription quantitative PCR. All three genes showed low levels of transcription when the cells were grown until exponential growth phase in the presence of common-bean-seed exudates or of the root nod-gene inducer naringenin. However, when cells at the exponential phase of growth were incubated with seed exudates, transcription occurred after only 5 min, and nodC, nodG, and glgX were transcribed 121.97-, 14.86-, and 50.29-fold more than the control, respectively, followed by a rapid decrease in gene transcription. Much lower levels of transcription were observed in the presence of naringenin; furthermore, maximum transcription required 8 h of incubation for all three genes. In light of these results, the mechanisms of induction of the nodulation genes by flavonoids are discussed.
650 $aBeans
650 $aGenes
650 $aNitrogen fixation
650 $aRhizobium tropici
650 $aSymbiosis
650 $aFeijao
650 $aFixacao de nitrogenio
650 $aGene
650 $aPhaseolus Vulgaris
650 $aSimbiose
653 $aReverse transcription quantitative-PCR
700 1 $aMARCELINO, F. C.
700 1 $aBARCELLOS, F. G.
700 1 $aRODRIGUES, E. P.
700 1 $aMEGIAS, M.
700 1 $aHUNGRIA, M.
773 $tFunctional & Integrative Genomics, Heidelberg$gv. 10, n. 3, p. 425-431, Aug. 2010.
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Registro original: |
Embrapa Soja (CNPSO) |
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