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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
19/03/2010 |
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Data da última atualização: |
28/05/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, L. R.; MARCELINO, F. C.; BARCELLOS, F. G.; RODRIGUES, E. P.; MEGIAS, M.; HUNGRIA, M. |
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Afiliação: |
LUCIANA RUANO OLIVEIRA, UEL; FRANCISMAR CORREA MARCELINO, CNPSO; FERNANDO GOMES BARCELLOS; ELISETE PAINS RODRIGUES; MANUEL MEGIAS, UNIVERSIDAD DE SEVILHA; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
The nodC, nodG, and glgX genes of Rhizobium tropici strain PRF 81. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Functional & Integrative Genomics, v. 10, n. 3, p. 425-431, Aug. 2010. |
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DOI: |
10.1007/s10142-009-0151-x |
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Idioma: |
Inglês |
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Conteúdo: |
Rhizobium tropici is a diazotrophic microsymbiont of common bean (Phaseolus vulgaris L.) that encompasses important but still poorly studied tropical strains, and a recent significant contribution to the knowledge of the species was the publication of a genomic draft of strain PRF 81, which revealed several novel genes [Pinto et al. Funct Int Gen 9:263?270, 2009]. In this study, we investigated the transcription of nodC, nodG, and glgX genes, located in the nod operon of PRF 81 strain, by reverse-transcription quantitative PCR. All three genes showed low levels of transcription when the cells were grown until exponential growth phase in the presence of common-bean-seed exudates or of the root nod-gene inducer naringenin. However, when cells at the exponential phase of growth were incubated with seed exudates, transcription occurred after only 5 min, and nodC, nodG, and glgX were transcribed 121.97-, 14.86-, and 50.29-fold more than the control, respectively, followed by a rapid decrease in gene transcription. Much lower levels of transcription were observed in the presence of naringenin; furthermore, maximum transcription required 8 h of incubation for all three genes. In light of these results, the mechanisms of induction of the nodulation genes by flavonoids are discussed. |
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Palavras-Chave: |
Reverse transcription quantitative-PCR. |
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Thesagro: |
Feijao; Fixacao de nitrogenio; Gene; Phaseolus Vulgaris; Simbiose. |
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Thesaurus Nal: |
Beans; Genes; Nitrogen fixation; Rhizobium tropici; Symbiosis. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02216naa a2200325 a 4500
001 1661862
005 2025-05-28
008 2010 bl uuuu u00u1 u #d
024 7 $a10.1007/s10142-009-0151-x$2DOI
100 1 $aOLIVEIRA, L. R.
245 $aThe nodC, nodG, and glgX genes of Rhizobium tropici strain PRF 81.$h[electronic resource]
260 $c2010
520 $aRhizobium tropici is a diazotrophic microsymbiont of common bean (Phaseolus vulgaris L.) that encompasses important but still poorly studied tropical strains, and a recent significant contribution to the knowledge of the species was the publication of a genomic draft of strain PRF 81, which revealed several novel genes [Pinto et al. Funct Int Gen 9:263?270, 2009]. In this study, we investigated the transcription of nodC, nodG, and glgX genes, located in the nod operon of PRF 81 strain, by reverse-transcription quantitative PCR. All three genes showed low levels of transcription when the cells were grown until exponential growth phase in the presence of common-bean-seed exudates or of the root nod-gene inducer naringenin. However, when cells at the exponential phase of growth were incubated with seed exudates, transcription occurred after only 5 min, and nodC, nodG, and glgX were transcribed 121.97-, 14.86-, and 50.29-fold more than the control, respectively, followed by a rapid decrease in gene transcription. Much lower levels of transcription were observed in the presence of naringenin; furthermore, maximum transcription required 8 h of incubation for all three genes. In light of these results, the mechanisms of induction of the nodulation genes by flavonoids are discussed.
650 $aBeans
650 $aGenes
650 $aNitrogen fixation
650 $aRhizobium tropici
650 $aSymbiosis
650 $aFeijao
650 $aFixacao de nitrogenio
650 $aGene
650 $aPhaseolus Vulgaris
650 $aSimbiose
653 $aReverse transcription quantitative-PCR
700 1 $aMARCELINO, F. C.
700 1 $aBARCELLOS, F. G.
700 1 $aRODRIGUES, E. P.
700 1 $aMEGIAS, M.
700 1 $aHUNGRIA, M.
773 $tFunctional & Integrative Genomics$gv. 10, n. 3, p. 425-431, Aug. 2010.
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Embrapa Soja (CNPSO) |
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| 5. |  | GUERRA, J. V. S.; SOARES, T. A. de S.; OLIVEIRA, S. M. de; SOUZA, T. L. P. O. de; ASPIAZÚ, I.; CARVALHO, A. J. de. Produtividade de grãos de linhagens-elite de feijão roxo no Norte de Minas Gerais. Ciclo de avaliação 2018-2019. In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 13., 2021, Goiânia. Conectividade tecnológica, intensificação sustentável: resumos. Brasília, DF: Embrapa; Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2021. p. 129. Evento online.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 6. | | TEIXEIRA, N. de C.; QUEIROZ, V. A. V.; ROCHA, M. C.; AMORIM, A. C. P.; SOARES, T. A.; MONTEIRO, M. A. M.; MENEZES, C. B. de; SCHAFFERT, R. E.; GARCIA, M. A. V. T.; JUNQUEIRA, R. G. Resistant starch content among several sorghum (Sorghum bicolor) genotypes and the effect of heat treatment on resistant starch retention in two genotypes. Food Chemistry, Essex, v. 197, p. 291-296, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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