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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
23/02/2010 |
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Data da última atualização: |
07/07/2025 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
MATSUMOTO, K.; COELHO, M. C. F.; MONTE, D. de C.; TEIXEIRA, J. B. |
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Afiliação: |
KAZUMITSU MATSUMOTO, CENARGEN; MARLY CATARINA FELIPE COELHO, CENARGEN; DAMARES DE CASTRO MONTE, CENARGEN; JOAO BATISTA TEIXEIRA, CENARGEN. |
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Título: |
Sterilization of non-autoclavable vessels and culture media by sodium hypochlorite for in vitro culture. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Acta Horticulturae, Leuven, n. 839, p. 329-335, 2009. |
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Idioma: |
Inglês |
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Notas: |
Edição de Proceedings of the 1st. International Symposium on Biotechnology of Fruit Species, Dresden, Germany, 2008. v. 1. |
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Conteúdo: |
As an alternative for sterilization by autoclaving, disinfection protocols for vessels and media using sodium hypochlorite (NaOCI) were developed for banana (Musa spp.) shoot and Agrobacterium tumefaciens cultures. The NaOCI concentration of 0.002% was sufficient to prevent microbial growth when the disinfested vessels were incubated with a modified MS medium for banana shoot cultures. However, instead of the MS medium, when they were incubated with a MYA medium for Agrobacterium cell cultures, bacterial contamination appeared, and a higher concentration of NaOCI was necessary to have an efficient disinfection. Culture media disinfected by NaOCl were successfully achieved for banana but not for Agrobacterium cell cultures. For plant tissue cultures, non-autoclavable vessels are suggested to be disinfected by the solution of 0.01% NaOCl at pH 5.4, and culture media by supplementing with 0.002% NaOCl. For Agrobacterium cell cultures, vessel disinfections by 0.01% NaOCl are suggested, but media could not be disinfected by the NaOCI supplementation. |
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Palavras-Chave: |
Desinfection. |
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Thesagro: |
Agrobacterium Tumefaciens; Ph. |
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Thesaurus Nal: |
micropropagation; Musa. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01832nam a2200217 a 4500
001 1658552
005 2025-07-07
008 2009 bl uuuu u00u1 u #d
100 1 $aMATSUMOTO, K.
245 $aSterilization of non-autoclavable vessels and culture media by sodium hypochlorite for in vitro culture.$h[electronic resource]
260 $aActa Horticulturae, Leuven, n. 839, p. 329-335, 2009.$c2009
500 $aEdição de Proceedings of the 1st. International Symposium on Biotechnology of Fruit Species, Dresden, Germany, 2008. v. 1.
520 $aAs an alternative for sterilization by autoclaving, disinfection protocols for vessels and media using sodium hypochlorite (NaOCI) were developed for banana (Musa spp.) shoot and Agrobacterium tumefaciens cultures. The NaOCI concentration of 0.002% was sufficient to prevent microbial growth when the disinfested vessels were incubated with a modified MS medium for banana shoot cultures. However, instead of the MS medium, when they were incubated with a MYA medium for Agrobacterium cell cultures, bacterial contamination appeared, and a higher concentration of NaOCI was necessary to have an efficient disinfection. Culture media disinfected by NaOCl were successfully achieved for banana but not for Agrobacterium cell cultures. For plant tissue cultures, non-autoclavable vessels are suggested to be disinfected by the solution of 0.01% NaOCl at pH 5.4, and culture media by supplementing with 0.002% NaOCl. For Agrobacterium cell cultures, vessel disinfections by 0.01% NaOCl are suggested, but media could not be disinfected by the NaOCI supplementation.
650 $amicropropagation
650 $aMusa
650 $aAgrobacterium Tumefaciens
650 $aPh
653 $aDesinfection
700 1 $aCOELHO, M. C. F.
700 1 $aMONTE, D. de C.
700 1 $aTEIXEIRA, J. B.
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| 1. |  | PINTO, J. F. S. K. C.; SETZER, A.; MORELLI, F.; GOMES, A. R.; ADAMI, M.; VENTURIERI, A.; GUIMARÃES, T. de F. P. L. Dinâmica do uso e cobertura do solo em áreas queimadas de municípios na Amazônia brasileira. In: SIMPÓSIO BRASILEIRO DE SENSORIAMENTO REMOTO, 18., 2017, Santos. Anais... São José dos Campos: INPE, 2017. p. 1353-1360.| Tipo: Artigo em Anais de Congresso |
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