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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
26/11/1999 |
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Data da última atualização: |
15/05/2023 |
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Autoria: |
MIRANDA, M.; MOTOMURA, T.; IKEDA, F.; OHGAWARA, T.; SAITO, W.; ENDO, T.; OMURA, M.; MORIGUCHI, T. |
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Título: |
Somatic hybrids obtained by fusion between Poncirus trifoliata (2x) and fortunella hindsii (4x) protoplasts. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Plant Cell Reports, v.16, p.401-405, 1997. |
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Idioma: |
Inglês |
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Conteúdo: |
Somatic hybrids were obtained by the symmetric fusion of embryogenic callus cells from tetraploid `Mame' kumquat [Fortunella hindsii (Champ.) Swing.] and mesophyll cells from diploid trifoliate orange [Poncirus trifoliata (L.) Raf.]. Southern blot analysis of three regenerants revealed that they carried specific rDNA fragments from both fusion partners, thereby confirming their hybridity. In contrast, mitochondrial DNA (mtDNA) and chloroblast DNA (cpDNA) were unidirectionally transmitted from the callus parent without any evidence of recombination. No differences in the restriction fragment patterns of rDNA, mtDNA or cpDNA could be detected among the regenerants. Flow cytometry showed that two regenerants were hexaploids, as expected, but that one was pentaploid, probably due to elimination of chromosomes prior to the regeneration of this plant. |
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Palavras-Chave: |
Citros; Eletrofusao; Eliminacao de cromossomos; Poliplaides. |
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Thesaurus Nal: |
chromosome elimination; Citrus; electrofusion; flow cytometry; polyploidy. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01714naa a2200313 a 4500
001 1648429
005 2023-05-15
008 1997 bl uuuu u00u1 u #d
100 1 $aMIRANDA, M.
245 $aSomatic hybrids obtained by fusion between Poncirus trifoliata (2x) and fortunella hindsii (4x) protoplasts.$h[electronic resource]
260 $c1997
520 $aSomatic hybrids were obtained by the symmetric fusion of embryogenic callus cells from tetraploid `Mame' kumquat [Fortunella hindsii (Champ.) Swing.] and mesophyll cells from diploid trifoliate orange [Poncirus trifoliata (L.) Raf.]. Southern blot analysis of three regenerants revealed that they carried specific rDNA fragments from both fusion partners, thereby confirming their hybridity. In contrast, mitochondrial DNA (mtDNA) and chloroblast DNA (cpDNA) were unidirectionally transmitted from the callus parent without any evidence of recombination. No differences in the restriction fragment patterns of rDNA, mtDNA or cpDNA could be detected among the regenerants. Flow cytometry showed that two regenerants were hexaploids, as expected, but that one was pentaploid, probably due to elimination of chromosomes prior to the regeneration of this plant.
650 $achromosome elimination
650 $aCitrus
650 $aelectrofusion
650 $aflow cytometry
650 $apolyploidy
653 $aCitros
653 $aEletrofusao
653 $aEliminacao de cromossomos
653 $aPoliplaides
700 1 $aMOTOMURA, T.
700 1 $aIKEDA, F.
700 1 $aOHGAWARA, T.
700 1 $aSAITO, W.
700 1 $aENDO, T.
700 1 $aOMURA, M.
700 1 $aMORIGUCHI, T.
773 $tPlant Cell Reports$gv.16, p.401-405, 1997.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 4 | |
| 1. |  | ASSIS, L. M. B.; NICOLETTI, N. A.; COELHO, M. S. S.; PEIXOTO, W. M.; BARBOSA JUNIOR, J. B.; ALMEIDA, R. G. de; BARROS, L. V. de; ABREU, J. G. de. Pod, straw and root mass after soybean physiological maturity in integrated systems. In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FORESTRY SYSTEMS: 100% DIGITAL, 2., 2021. WCCLF 2021 proceedings. Campo Grande, MS: Embrapa Gado de Corte, 2021. p. 392-395.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 2. | | ASSIS, L. M. B.; COELHO, M. S. S.; PEIXOTO. W. M.; NICOLETTI, N. A.; BARBOSA JUNIOR, J. B.; ALMEIDA, R. G. de; BARROS, L. V. de; ABREU, J. G. de. Soybean height and yield in and integrated system with Eucalyptus. In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FORESTRY SYSTEMS: 100% DIGITAL, 2., 2021. WCCLF 2021 proceedings. Campo Grande, MS: Embrapa Gado de Corte, 2021. p. 685-689. WCCLF. Evento online.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 3. | | MOREIRA G. M.; ABREU, J. G. de; ALMEIDA, R. G. de; BARROS, L. V. de; AZEVEDO, V. H. de; SILVA NETO, I. M. da; CABRAL, C. E. A.; HERRERA, L. D. S.; PEIXOTO, W. M.; HERRERA, D. M. Sorghum intercropped with Piatã Grass in eucalyptus sub-forest. Journal of Experimental Agriculture International, v. 37, n. 1, p.1-9, 2019, article JEAI.49086.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 4. | | FAVARE, H. G. de; ABREU, J. G. de; BARROS, L. V. de; SILVA, F. G. da; FERREIRA, L. M. M.; BARELLI, M. A. A.; SILVA NETO, I. M. da; CABRAL, C. E. A.; PEIXOTO, W. M.; CAMPOS, F. I. da S.; LEDO, F. J. da S.; SILVA, V. Q. R. da; HERRERA, L. D. S. Effect of Elephant Grass Genotypes to Bioenergy Production. Journal of Experimental Agriculture International, v. 38, n. 1, article JEAI.49640, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Agrossilvipastoril; Embrapa Gado de Leite. |
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| Registros recuperados : 4 | |
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