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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
14/08/1995 |
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Data da última atualização: |
17/05/2023 |
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Autoria: |
REUVENI, O.; SHLESINGER, D. R.; LAVI, U. |
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Afiliação: |
Institute Horticulture, Agricultural Research Organization, the Volcani Center, Israel. |
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Título: |
In Vitro Clonal Propagation of Diocious Carica Papaya. |
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Ano de publicação: |
1990 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, v.20, p.41-46, 1990. |
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Idioma: |
Espanhol |
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Conteúdo: |
A procedure for in vitro propagation of dioecious papaya clones is described. A high rate of sucess in culture estbalishment was obtained when axiliary buds were taken from lateral shoots of hedged rooted cuttings grown in a greenhouse. Seasonal endophytic contamination was suppressed by shaking propagules for 24 h in 300 mgl rifampicin or by incorporating it at 50 mgl into the medium. Murashige & Skoog (MS) basal medium supplemented with 0.5 mgl 6-benzyladenine and 0.1mgl adenine sulfate improved multiplication and shoot growth. An elongation stage on MS medium supplemented with 1.0 mgl kinetin and 0.05 mgl naphthaleneacetic acid was necessary before rooting. Rooting was obtained at a high rate on half-strength macroelements of MS medium supplemented with 1.0mgl indole-3-butyric acid. Commercial plots of papya plants obtained through this procedure already exist. |
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Palavras-Chave: |
Endophytic contamination; Papaya. |
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Thesaurus Nal: |
micropropagation; rifampicin; tissue culture. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01447naa a2200205 a 4500
001 1646732
005 2023-05-17
008 1990 bl uuuu u00u1 u #d
100 1 $aREUVENI, O.
245 $aIn Vitro Clonal Propagation of Diocious Carica Papaya.$h[electronic resource]
260 $c1990
520 $aA procedure for in vitro propagation of dioecious papaya clones is described. A high rate of sucess in culture estbalishment was obtained when axiliary buds were taken from lateral shoots of hedged rooted cuttings grown in a greenhouse. Seasonal endophytic contamination was suppressed by shaking propagules for 24 h in 300 mgl rifampicin or by incorporating it at 50 mgl into the medium. Murashige & Skoog (MS) basal medium supplemented with 0.5 mgl 6-benzyladenine and 0.1mgl adenine sulfate improved multiplication and shoot growth. An elongation stage on MS medium supplemented with 1.0 mgl kinetin and 0.05 mgl naphthaleneacetic acid was necessary before rooting. Rooting was obtained at a high rate on half-strength macroelements of MS medium supplemented with 1.0mgl indole-3-butyric acid. Commercial plots of papya plants obtained through this procedure already exist.
650 $amicropropagation
650 $arifampicin
650 $atissue culture
653 $aEndophytic contamination
653 $aPapaya
700 1 $aSHLESINGER, D. R.
700 1 $aLAVI, U.
773 $tPlant Cell, Tissue and Organ Culture$gv.20, p.41-46, 1990.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
