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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
05/07/1995 |
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Data da última atualização: |
12/04/2023 |
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Autoria: |
SCHUMACHER, J.; RANDLES, J. W.; RIESNER D. |
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Afiliação: |
Institut fur Physikalische Biologie, Universitat Dusseldorf, Universitatsstrasse 1, D-4000 Dusseldorf, West Germany Received March 30, 1983. |
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Título: |
A two-dimensional electrophoretic technique for the detection of circular viroids and virusoids. |
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Ano de publicação: |
1983 |
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Fonte/Imprenta: |
Analytical Biochemistry, v.135, p.288-295, 1983. |
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Idioma: |
Inglês |
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Conteúdo: |
A mew gel electrophoretic technique for the rapid and sensitive detection of circular viroids and virusoids is described. Starting from plant material, a typical multisample analysis requires less than 8 h. Viroid concentrations as low as 60 ng/g tissue can be detected unambiguously without the use of radioactivity or highly specialized laboratory equipament. The technique presented here is compared to earlier methods of gel electrophoresis, nucleic acid fingerprinting, and currently employed hybridization techniques. A number of important technical advantages, including speed, simplicity, and sensitivity, suggest that the methods described here may have wide utility in checking the spread of viroid infections. |
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Palavras-Chave: |
2-D Electrophoresis; Pathogen; Virusoids. |
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Thesaurus Nal: |
detection; viroids. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01307naa a2200205 a 4500
001 1646625
005 2023-04-12
008 1983 bl uuuu u00u1 u #d
100 1 $aSCHUMACHER, J.
245 $aA two-dimensional electrophoretic technique for the detection of circular viroids and virusoids.$h[electronic resource]
260 $c1983
520 $aA mew gel electrophoretic technique for the rapid and sensitive detection of circular viroids and virusoids is described. Starting from plant material, a typical multisample analysis requires less than 8 h. Viroid concentrations as low as 60 ng/g tissue can be detected unambiguously without the use of radioactivity or highly specialized laboratory equipament. The technique presented here is compared to earlier methods of gel electrophoresis, nucleic acid fingerprinting, and currently employed hybridization techniques. A number of important technical advantages, including speed, simplicity, and sensitivity, suggest that the methods described here may have wide utility in checking the spread of viroid infections.
650 $adetection
650 $aviroids
653 $a2-D Electrophoresis
653 $aPathogen
653 $aVirusoids
700 1 $aRANDLES, J. W.
700 1 $aRIESNER D.
773 $tAnalytical Biochemistry$gv.135, p.288-295, 1983.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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