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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
05/07/1995 |
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Data da última atualização: |
12/04/2023 |
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Autoria: |
SCHUMACHER, J.; RANDLES, J. W.; RIESNER D. |
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Afiliação: |
Institut fur Physikalische Biologie, Universitat Dusseldorf, Universitatsstrasse 1, D-4000 Dusseldorf, West Germany Received March 30, 1983. |
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Título: |
A two-dimensional electrophoretic technique for the detection of circular viroids and virusoids. |
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Ano de publicação: |
1983 |
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Fonte/Imprenta: |
Analytical Biochemistry, v.135, p.288-295, 1983. |
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Idioma: |
Inglês |
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Conteúdo: |
A mew gel electrophoretic technique for the rapid and sensitive detection of circular viroids and virusoids is described. Starting from plant material, a typical multisample analysis requires less than 8 h. Viroid concentrations as low as 60 ng/g tissue can be detected unambiguously without the use of radioactivity or highly specialized laboratory equipament. The technique presented here is compared to earlier methods of gel electrophoresis, nucleic acid fingerprinting, and currently employed hybridization techniques. A number of important technical advantages, including speed, simplicity, and sensitivity, suggest that the methods described here may have wide utility in checking the spread of viroid infections. |
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Palavras-Chave: |
2-D Electrophoresis; Pathogen; Virusoids. |
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Thesaurus Nal: |
detection; viroids. |
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Categoria do assunto: |
-- |
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Marc: |
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001 1646625
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008 1983 bl uuuu u00u1 u #d
100 1 $aSCHUMACHER, J.
245 $aA two-dimensional electrophoretic technique for the detection of circular viroids and virusoids.$h[electronic resource]
260 $c1983
520 $aA mew gel electrophoretic technique for the rapid and sensitive detection of circular viroids and virusoids is described. Starting from plant material, a typical multisample analysis requires less than 8 h. Viroid concentrations as low as 60 ng/g tissue can be detected unambiguously without the use of radioactivity or highly specialized laboratory equipament. The technique presented here is compared to earlier methods of gel electrophoresis, nucleic acid fingerprinting, and currently employed hybridization techniques. A number of important technical advantages, including speed, simplicity, and sensitivity, suggest that the methods described here may have wide utility in checking the spread of viroid infections.
650 $adetection
650 $aviroids
653 $a2-D Electrophoresis
653 $aPathogen
653 $aVirusoids
700 1 $aRANDLES, J. W.
700 1 $aRIESNER D.
773 $tAnalytical Biochemistry$gv.135, p.288-295, 1983.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| 1. |  | LOBO-JR, A. R.; DELGADO, E. F.; MOURÃO, G. B.; PEDREIRA, A. C. M. S.; BERNDT, A.; DEMARCHI, J. J. A. A. Interaction of dietary vitamin D3 and sunlight exposure on B. indicus cattle: animal performance, carcass traits, and meat quality. Livestock Science, v. 145, n. 1-3, p. 196-204, may 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 2. | | SANTOS, S. A.; MAZZA, M. C. M.; SERENO, J. R. B.; PEDREIRA, A. C. M. S.; MARIANTE, A. A. S.; SILVA, J. A.; MARQUES, M. C. de. Sistema de criação de cavalos pantaneiros no Pantanal. Archivos de Zootecnia, v. 53, p. 333-336, 2004.| Biblioteca(s): Embrapa Pantanal. |
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| 3. | | SANTOS, S. A.; MAZZA, M. C. M.; SERENO, J. R. B.; PEDREIRA, A. C. M. S.; MARIANTE, A. da S.; COMASTRI FILHO, J. A.; SILVA, J. A. da; MARQUES, M. C. de A. Descrição do manejo geral de cavalos pantaneiros na região do Pantanal. Corumbá: Embrapa Pantanal, 2005. 20 p. (Embrapa Pantanal. Boletim de Pesquisa e Desenvolvimento, 63).| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Pantanal. |
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| Registros recuperados : 3 | |
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