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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
06/05/2008 |
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Data da última atualização: |
16/03/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RODRIGUES, E. P.; RODRIGUES, L. da S.; OLIVEIRA, A. L. M. de; BALDANI, V. L. D.; TEIXEIRA, K. R. dos S.; URQUIAGA, S.; REIS, V. M. |
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Afiliação: |
Elisete Pains Rodrigues, UFRRJ; Luciana da Silva Rodrigues, UFRRJ; André Luiz Martinez de Oliveira, UFRRJ; Vera Lúcia Divan Baldani, Embrapa Agrobiologia; Kátia Regina dos Santos Teixeira, Embrapa Agrobiologia; Segundo Urquiaga, Embrapa Agrobiologia; Veronica Massena Reis, Embrapa Agrobiologia. |
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Título: |
Azospirillum amazonense inoculation: effects on growth, yield and N2 fixation of rice (Oryza sativa L.). |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Plant and Soil, The Netherlands, v. 302, n. 1-2, p. 249-261, jan. 2008. |
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Idioma: |
Inglês |
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Notas: |
Parceria: UFRRJ. |
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Conteúdo: |
Bacteria of the genus Azospirillum are considered to be plant-growth promoting bacteria (PGPR) and stimulate plant growth directly either by synthesising phyto-hormones or by promoting nutrition by the process of biological nitrogen fixation (BNF). Although this genus extensively studied, the effects of inoculation and the possible BNF contribution of the Azospirillum amazonense specie are very scarce. The aim of this study was to isolate, characterise and evaluate auxin production and nitrogenase activity of this species and to select, by inoculation of rice plants, promising isolates based on their ability to improve plant growth, yield and the BNF contribution. One hundred and ten isolates obtained ftom rice were characterised and grouped according to colony features. Forty-two isolates, confirmed as A. amazonense by the fluorescent in situ hybridization (FISH) technique, were tested for auxin production and nitrogenase activity in vitro. Subsequently plant growth promotion related to plant nutrition effect was evaluated, in vitro and in greenhouse experiments. The BNF contribution to plant growth was evaluated using the 15N isotope dilution technique. All A. amazonense strains tested produced indoles, but only 10% of them showed high production, above 1.33 µM mg protein-1. The nitro-genase activity also was variable and only 9% of isolates showed high nitrogenase activity and the majority (54%) exhibited a low potential. The inocu-lation of selected strains in rice under gnotobiotic conditions reduced the growth of root and aerial part when compared to the control, showing the negative effects of excess of phytohormone accumulation in the medium. However, in the greenhouse experiment, inoculation of strains of A. amazonense increased grain dry matter accumulation (7 to 11.6%), the number of panicles (3 to 18.6%) and nitrogen accumulation at grain maturation (3.5 to 18.5%). BNF contributions up to 27% were observed for A. amazonense Y2 (wild type strain). The data presented here is the first report that the PGPR effect of A. amazonense for rice plants grown under greenhouse conditions is mainly due the BNF contribution as measured by 15N isotope dilution technique, in contrast to the hormonal effect observed with other Azospirillum species studied. MenosBacteria of the genus Azospirillum are considered to be plant-growth promoting bacteria (PGPR) and stimulate plant growth directly either by synthesising phyto-hormones or by promoting nutrition by the process of biological nitrogen fixation (BNF). Although this genus extensively studied, the effects of inoculation and the possible BNF contribution of the Azospirillum amazonense specie are very scarce. The aim of this study was to isolate, characterise and evaluate auxin production and nitrogenase activity of this species and to select, by inoculation of rice plants, promising isolates based on their ability to improve plant growth, yield and the BNF contribution. One hundred and ten isolates obtained ftom rice were characterised and grouped according to colony features. Forty-two isolates, confirmed as A. amazonense by the fluorescent in situ hybridization (FISH) technique, were tested for auxin production and nitrogenase activity in vitro. Subsequently plant growth promotion related to plant nutrition effect was evaluated, in vitro and in greenhouse experiments. The BNF contribution to plant growth was evaluated using the 15N isotope dilution technique. All A. amazonense strains tested produced indoles, but only 10% of them showed high production, above 1.33 µM mg protein-1. The nitro-genase activity also was variable and only 9% of isolates showed high nitrogenase activity and the majority (54%) exhibited a low potential. The inocu-lation of selected strains in rice under... Mostrar Tudo |
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Palavras-Chave: |
Biological nitrogen fixation; BNF; FBN; Fixação biológica de nitrogênio; Growth. |
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Thesagro: |
Arroz; Crescimento; Oryza Sativa. |
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Thesaurus Nal: |
Azospirillum amazonense; rice. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03231naa a2200325 a 4500
001 1629942
005 2015-03-16
008 2008 bl --- 0-- u #d
100 1 $aRODRIGUES, E. P.
245 $aAzospirillum amazonense inoculation$beffects on growth, yield and N2 fixation of rice (Oryza sativa L.).
260 $c2008
500 $aParceria: UFRRJ.
520 $aBacteria of the genus Azospirillum are considered to be plant-growth promoting bacteria (PGPR) and stimulate plant growth directly either by synthesising phyto-hormones or by promoting nutrition by the process of biological nitrogen fixation (BNF). Although this genus extensively studied, the effects of inoculation and the possible BNF contribution of the Azospirillum amazonense specie are very scarce. The aim of this study was to isolate, characterise and evaluate auxin production and nitrogenase activity of this species and to select, by inoculation of rice plants, promising isolates based on their ability to improve plant growth, yield and the BNF contribution. One hundred and ten isolates obtained ftom rice were characterised and grouped according to colony features. Forty-two isolates, confirmed as A. amazonense by the fluorescent in situ hybridization (FISH) technique, were tested for auxin production and nitrogenase activity in vitro. Subsequently plant growth promotion related to plant nutrition effect was evaluated, in vitro and in greenhouse experiments. The BNF contribution to plant growth was evaluated using the 15N isotope dilution technique. All A. amazonense strains tested produced indoles, but only 10% of them showed high production, above 1.33 µM mg protein-1. The nitro-genase activity also was variable and only 9% of isolates showed high nitrogenase activity and the majority (54%) exhibited a low potential. The inocu-lation of selected strains in rice under gnotobiotic conditions reduced the growth of root and aerial part when compared to the control, showing the negative effects of excess of phytohormone accumulation in the medium. However, in the greenhouse experiment, inoculation of strains of A. amazonense increased grain dry matter accumulation (7 to 11.6%), the number of panicles (3 to 18.6%) and nitrogen accumulation at grain maturation (3.5 to 18.5%). BNF contributions up to 27% were observed for A. amazonense Y2 (wild type strain). The data presented here is the first report that the PGPR effect of A. amazonense for rice plants grown under greenhouse conditions is mainly due the BNF contribution as measured by 15N isotope dilution technique, in contrast to the hormonal effect observed with other Azospirillum species studied.
650 $aAzospirillum amazonense
650 $arice
650 $aArroz
650 $aCrescimento
650 $aOryza Sativa
653 $aBiological nitrogen fixation
653 $aBNF
653 $aFBN
653 $aFixação biológica de nitrogênio
653 $aGrowth
700 1 $aRODRIGUES, L. da S.
700 1 $aOLIVEIRA, A. L. M. de
700 1 $aBALDANI, V. L. D.
700 1 $aTEIXEIRA, K. R. dos S.
700 1 $aURQUIAGA, S.
700 1 $aREIS, V. M.
773 $tPlant and Soil, The Netherlands$gv. 302, n. 1-2, p. 249-261, jan. 2008.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
26/10/2015 |
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Data da última atualização: |
26/10/2015 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
VIANNA, G. R.; ARAGAO, F. J. L.; RECH, E. L. |
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Afiliação: |
GIOVANNI RODRIGUES VIANNA, CENARGEN; FRANCISCO JOSE LIMA ARAGAO, CENARGEN; ELIBIO LEOPOLDO RECH FILHO, CENARGEN. |
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Título: |
O sistema biobalístico. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: BRASILEIRO, A. C. M.; CARNEIRO, V. T. de C. (Ed.). Manual de transformação genética de plantas. 2.ed. rev. e ampl. Brasília, DF: Embrapa, 2015. p. 89-104. |
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Idioma: |
Português |
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Palavras-Chave: |
Bombardeamento; Esterilização e precipitação de DNA sobre os micropartículas. |
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Thesagro: |
Algodão; Feijão; Planta transgênica; Soja. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00673naa a2200205 a 4500
001 2027150
005 2015-10-26
008 2015 bl uuuu u00u1 u #d
100 1 $aVIANNA, G. R.
245 $aO sistema biobalístico.
260 $c2015
650 $aAlgodão
650 $aFeijão
650 $aPlanta transgênica
650 $aSoja
653 $aBombardeamento
653 $aEsterilização e precipitação de DNA sobre os micropartículas
700 1 $aARAGAO, F. J. L.
700 1 $aRECH, E. L.
773 $tIn: BRASILEIRO, A. C. M.; CARNEIRO, V. T. de C. (Ed.). Manual de transformação genética de plantas. 2.ed. rev. e ampl. Brasília, DF: Embrapa, 2015. p. 89-104.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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