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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
08/01/2010 |
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Data da última atualização: |
15/01/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
FERRARI, F.; ZANCA, A. S.; JORDÃO, H.; GIACHETTO, P. F.; OKURA, V. K.; MACCHERONI, W.; FORNI-MARTINS, E. R. |
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Afiliação: |
FERNANDA FERRARI, CanaVialis S.A., IB/Unicamp; A. S. ZANCA, CanaVialis S.A.; H. JORDÃO, CanaVialis S.A.; POLIANA FERNANDA GIACHETTO, CNPTIA; V. K. OKURA, CanaVialis S.A.; W. MACCHERONI, CanaVialis S.A.; E. R. FORNI-MARTINS, IB/Unicamp. |
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Título: |
Sugarcane BAC selection for BAC-FISH in currents hybrids varieties. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia, SP. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009. |
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Páginas: |
p. 96. |
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Idioma: |
Inglês |
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Conteúdo: |
In order to better understand the organization and support sugarcane breeding programs we are beginning a construction of a physical map using BAC-FISH. We began the study with six clones BACs available at Alellyx S.A. These BACs were pre-selected for specific promoters search project. Filters of the sugarcane BAC library and the clones were obtained from Clemson University Genomics Institutes (CUGI, Clemson, South Carolina). This BAC library was constructed from genomic DNA of the hybrid 'R570. BAC DNA was shotgun sub cloned and was sequenced using BigDye system and ABI Prism 3730 DNA Sequencer (Applied Biosystems). Sequence homology was determined by BLAST searches of the nucleotide sequence database at the National Center for Biotechnology Information (NCBI). We analyzed the BLAST results and we could classify these six BACs when compared with sorghum genome database. |
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Palavras-Chave: |
BAC library; BAC-FISH. |
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Thesagro: |
Cana de açúcar; Genética. |
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Thesaurus Nal: |
Breeding; Genetics; Physical chromosome mapping; Sugarcane. |
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Categoria do assunto: |
-- |
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Marc: |
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001 1580031
005 2020-01-15
008 2009 bl uuuu u00u1 u #d
100 1 $aFERRARI, F.
245 $aSugarcane BAC selection for BAC-FISH in currents hybrids varieties.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia, SP. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética$c2009
300 $ap. 96.
520 $aIn order to better understand the organization and support sugarcane breeding programs we are beginning a construction of a physical map using BAC-FISH. We began the study with six clones BACs available at Alellyx S.A. These BACs were pre-selected for specific promoters search project. Filters of the sugarcane BAC library and the clones were obtained from Clemson University Genomics Institutes (CUGI, Clemson, South Carolina). This BAC library was constructed from genomic DNA of the hybrid 'R570. BAC DNA was shotgun sub cloned and was sequenced using BigDye system and ABI Prism 3730 DNA Sequencer (Applied Biosystems). Sequence homology was determined by BLAST searches of the nucleotide sequence database at the National Center for Biotechnology Information (NCBI). We analyzed the BLAST results and we could classify these six BACs when compared with sorghum genome database.
650 $aBreeding
650 $aGenetics
650 $aPhysical chromosome mapping
650 $aSugarcane
650 $aCana de açúcar
650 $aGenética
653 $aBAC library
653 $aBAC-FISH
700 1 $aZANCA, A. S.
700 1 $aJORDÃO, H.
700 1 $aGIACHETTO, P. F.
700 1 $aOKURA, V. K.
700 1 $aMACCHERONI, W.
700 1 $aFORNI-MARTINS, E. R.
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Embrapa Agricultura Digital (CNPTIA) |
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