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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
16/04/2008 |
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Data da última atualização: |
17/01/2024 |
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Autoria: |
MATOS, M. H. T.; HURK, R. van den; MARTINS, F. S.; SANTOS, R. R.; LUQUE, M. C. A.; SILVA, J. R. V.; CELESTINO, J. J. H.; BÁO, S. N.; FIGUEIREDO, J. R. |
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Título: |
Histological and ulrastructural features of aprine preantral follicles after in vitro culture in the presence or absence of indole-3-acetic acid. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Animal Reproduction, v. 3, n. 4, p. 415-422, Oct./Dec. 2006. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of the present study was to evaluate the effects of indole-3-acetic acid (IAA) on survival, activation, and growth of caprine preantral follicles using histological and ultrastructural criteria. Pieces of caprine ovarian cortex were cultured for 1 or 5 days in Minimum Essential Medium (MEM - control medium) supplemented with different concentrations of IAA (10, 20, 40, or 100 ng/ml). Small fragments from non-cultured ovarian tissue and from those cultured for 1 or 5 days in a specific medium were processed for classical histology and ultrastructural analysis. Follicle and oocyte growth were evaluated before and after 1 or 5 days of culture in the various media tested. The results showed a greater percentage of histologically-normal follicles in MEM alone or MEM supplemented with IAA (20 ng/ml) than other treatments. Indole-3-acetic acid added at a concentration of 20 or 40 ng/ml increased the proportion of primordial follicles that entered the growth phase after 5 days. In the presence of 10 or 20 ng/ml IAA, the follicles increased in diameter after 5 days of culture. Ultrastructural studies, however, did not confirm maintenance of the morphological integrity of caprine follicles cultured for 1 or 5 days in MEM supplemented with 20 ng/ml of IAA as was demonstrated by histological studies but in contrast showed distinct degenerative changes in both oocytes and granulosa cells. In conclusion, ultrastructural integrity of caprine preantral follicles can be successfully maintained after in vitro culture for 5 days in MEM without addition of IAA. In addition, ultrastructural analysis is necessary to judge the morphology of cultured preantral follicles since IAA negatively affects the ultrastructural composition of in vitro activated follicles, which was not detected by classical histology. MenosThe aim of the present study was to evaluate the effects of indole-3-acetic acid (IAA) on survival, activation, and growth of caprine preantral follicles using histological and ultrastructural criteria. Pieces of caprine ovarian cortex were cultured for 1 or 5 days in Minimum Essential Medium (MEM - control medium) supplemented with different concentrations of IAA (10, 20, 40, or 100 ng/ml). Small fragments from non-cultured ovarian tissue and from those cultured for 1 or 5 days in a specific medium were processed for classical histology and ultrastructural analysis. Follicle and oocyte growth were evaluated before and after 1 or 5 days of culture in the various media tested. The results showed a greater percentage of histologically-normal follicles in MEM alone or MEM supplemented with IAA (20 ng/ml) than other treatments. Indole-3-acetic acid added at a concentration of 20 or 40 ng/ml increased the proportion of primordial follicles that entered the growth phase after 5 days. In the presence of 10 or 20 ng/ml IAA, the follicles increased in diameter after 5 days of culture. Ultrastructural studies, however, did not confirm maintenance of the morphological integrity of caprine follicles cultured for 1 or 5 days in MEM supplemented with 20 ng/ml of IAA as was demonstrated by histological studies but in contrast showed distinct degenerative changes in both oocytes and granulosa cells. In conclusion, ultrastructural integrity of caprine preantral follicles can be successfully ... Mostrar Tudo |
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Palavras-Chave: |
Ácido acético; Ácido-3-indol acético; Foliculo pre-antral; Oocito; Ultraestrutura. |
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Thesagro: |
Caprino; Reprodução. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02748naa a2200301 a 4500
001 1533908
005 2024-01-17
008 2006 bl uuuu u00u1 u #d
100 1 $aMATOS, M. H. T.
245 $aHistological and ulrastructural features of aprine preantral follicles after in vitro culture in the presence or absence of indole-3-acetic acid.$h[electronic resource]
260 $c2006
520 $aThe aim of the present study was to evaluate the effects of indole-3-acetic acid (IAA) on survival, activation, and growth of caprine preantral follicles using histological and ultrastructural criteria. Pieces of caprine ovarian cortex were cultured for 1 or 5 days in Minimum Essential Medium (MEM - control medium) supplemented with different concentrations of IAA (10, 20, 40, or 100 ng/ml). Small fragments from non-cultured ovarian tissue and from those cultured for 1 or 5 days in a specific medium were processed for classical histology and ultrastructural analysis. Follicle and oocyte growth were evaluated before and after 1 or 5 days of culture in the various media tested. The results showed a greater percentage of histologically-normal follicles in MEM alone or MEM supplemented with IAA (20 ng/ml) than other treatments. Indole-3-acetic acid added at a concentration of 20 or 40 ng/ml increased the proportion of primordial follicles that entered the growth phase after 5 days. In the presence of 10 or 20 ng/ml IAA, the follicles increased in diameter after 5 days of culture. Ultrastructural studies, however, did not confirm maintenance of the morphological integrity of caprine follicles cultured for 1 or 5 days in MEM supplemented with 20 ng/ml of IAA as was demonstrated by histological studies but in contrast showed distinct degenerative changes in both oocytes and granulosa cells. In conclusion, ultrastructural integrity of caprine preantral follicles can be successfully maintained after in vitro culture for 5 days in MEM without addition of IAA. In addition, ultrastructural analysis is necessary to judge the morphology of cultured preantral follicles since IAA negatively affects the ultrastructural composition of in vitro activated follicles, which was not detected by classical histology.
650 $aCaprino
650 $aReprodução
653 $aÁcido acético
653 $aÁcido-3-indol acético
653 $aFoliculo pre-antral
653 $aOocito
653 $aUltraestrutura
700 1 $aHURK, R. van den
700 1 $aMARTINS, F. S.
700 1 $aSANTOS, R. R.
700 1 $aLUQUE, M. C. A.
700 1 $aSILVA, J. R. V.
700 1 $aCELESTINO, J. J. H.
700 1 $aBÁO, S. N.
700 1 $aFIGUEIREDO, J. R.
773 $tAnimal Reproduction$gv. 3, n. 4, p. 415-422, Oct./Dec. 2006.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 8 | |
| 1. |  | FRIES, D. D.; ALVES, J. D.; DELÚ FILHO, N.; MAGALHAES, P. C.; GOULART, P. de F. P.; MAGALHAES, M. M. Crescimento de plântulas do milho 'Saracura' e atividade de a-amilase e invertases associados ao aumento da tolerância ao alagamento exercido pelo cálcio exógeno. Bragantia, Campinas, v. 66, n. 1, p. 1-9, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - C |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 2. | | FERRER, J. L. R.; ALVES, J. D.; MAGALHAES, P. C.; DELU FILHO, N.; FRIES, D. D.; DURAES, F. O. M.; PURCINO, A. A. C. Aplicação de cálcio alivia parcialmente o efeito deletério da hipoxia em uma cultivar de milho selecionada para tolerância ao encharcamento. Brazilian Journal of Plant Physiology, Campinas, v. 15, p. 371, 2003. Suplemento. Edição dos resumos do IX Congresso Brasileiro de Fisiologia Vegetal, 2003, Atibaia, SP.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 3. | | FERRER, J. L. R.; ALVES, J. D.; MAGALHAES, P. C.; DELU FILHO, N.; FRIES, D.; MAGALHAES, M.; PURCINO, A. A. C. A aplicação de cálcio alivia parcialmente o efeito deletério da hipoxia em uma linhagem de milho selecionada para tolerância ao encharcamento. In: CONGRESSO NACIONAL DE MILHO E SORGO, 25.; SIMPOSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 1., 2004, Cuiabá, MT. Da agricultura familiar ao agronegócio: tecnologia, competitividade e sustentabilidade: [resumos expandidos]. Sete Lagoas: ABMS: Embrapa Milho e Sorgo; Cuiabá: Empaer, 2004. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 4. | | FERRER, J. L. R.; MAGALHAES, P. C.; ALVES, J. D.; VASCONCELLOS, C. A.; DELU FILHO, N.; FRIES, D. D.; MAGALHAES, M. M.; PURCINO, A. A. C. Calcium partially relieves the deleterius effects of hypoxia on a maize cultivar selected for waterlogging tolerance. Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 4, n. 3, p. 381-389, 2005.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 5. | | VIEIRA, C. V.; FERRER, J. R.; CASTRO, E. M.; ALVES, J. D.; MAGALHAES, P. C.; FRIES, D. D.; ALENCAR, M. A. Efeito do cálcio sobre as caracteristicas anatômicas de raízes de milho (Zea mays L.) "Saracura" BRS-4154 submetido ao alongamento em casa de vegetação. Brazilian Journal of Plant Physiology, Campinas, v.15, p. 395, 2003. Suplemento. Edição dos resumos do IX Congresso Brasileiro de Fisiologia Vegetal, 2003, Atibaia, SP.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 6. | | MELO, H. C. de; CASTRO, E. M. de; ROMERO, J. L.; ALVES, J. D.; FRIES, D. D.; MELO, L. A. de; MAGALHAES, P. C. Influência da aplicação de cálcio e alagamento do solo sobre características anatômicas das folhas de milho (Zea mays L.) "Saracura" BRS-4154. Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 3, n. 3, p. 333-342, 2004.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 7. | | RAMOS, B. L. P.; PEDREIRA, M. dos S.; SANTOS, H. P. dos; CRUZ, N. T.; PEZENTI, E.; SILVA, A. S.; DIAVÃO, J.; MORENZ, M. J. F.; PITTA, B. S. L.; FRIES, D. D. Forage production, morphogenetic and structural components, and nutritional value of tropical grasses in the semiarid condition. Semina: Ciências Agrárias, v. 43, n. 6, p. 2499-2516, nov./dez. 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 8. | | ALVES, J. D.; MAGALHAES, M. M.; GOULART, P. de F. P.; DANTAS, B. F.; GOUVEA, J. A. de; PURCINO, R. P.; MAGALHAES, P. C.; FRIES, D. D.; LIVRAMENTO, D. E. do; MEYER, L. E.; SEIFFERT, M.; SILVEIRA, T. Mecanismos de tolerância da variedade de milho Saracura (BRS 4154) ao alagamento. Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 1, n. 1, p.41-52, jan./abr. 2002.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - C |
| Biblioteca(s): Embrapa Milho e Sorgo; Embrapa Semiárido. |
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| Registros recuperados : 8 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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