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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
19/11/2003 |
Data da última atualização: |
28/08/2023 |
Autoria: |
LEONI, G.; BOGLIOLO, L.; PINTUS, P.; LEDDA, S.; NAITANA, S. |
Título: |
Sheep embryos derived from FSH/eCG treatment have a lower in vitro viability after vitrification than those derived from FSH treatment. |
Ano de publicação: |
2001 |
Fonte/Imprenta: |
Reproduction, Nutrition, Development, v. 41, n. 3, p. 239-246, May./Jun. 2001. |
DOI: |
10.1051/rnd:2001127 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: In the non breeding period, the effect of two superovulatory treatments (eCG/FSH in single dose or FSH alone in four decreasing doses) on the production of embryo quality following in vitro viability after vitrification procedures was investigated using forty-four adult Sarda breed ewes. In sheep treated with eCG/FSH, the mean number of corpora lutea was significantly (P < 0.05) higher (11.8+/-4.0 vs. 8.05+/-3.8), although the recovery rate was significantly (P < 0.01) lower (74.6 vs. 59.9) than with FSH alone. After vitrification (ethylene glycol and glycerol) was repeated three times, the rates of re-expansion at first and second warming were significantly (P < 0.01) higher in embryos derived from FSH alone than in those with both gonadotrophins (94.9 and 41.9 vs. 72.8 and 18.6) and after the last vitrification the hatched blastocyst rates were 22.5 and 7.6. After differential stain, blastocysts derived from FSH alone showed a mean number of cells significantly higher than blastocysts from eCG/FSH (184.2 vs. 157.7). It was concluded that superovulatory treatment with eCG/FSH may increase the ovarian responses compared with FSH alone, but these embryos showed a reduction in viability rates after repeated vitrification. |
Palavras-Chave: |
Cryoprotective agents; Drug effects; eCG; Embryo quality; FSH; In Vitro Techniques; Vitrificação. |
Thesaurus Nal: |
Cryopreservation; Embryo transfer; Ethylene; Ethylene glycol; Follicle-stimulating hormone; Glycerol; Pharmacology; Pregnancy; Reproduction; Sheep; Superovulation; Vitrification. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02426naa a2200409 a 4500 001 1530039 005 2023-08-28 008 2001 bl uuuu u00u1 u #d 024 7 $a10.1051/rnd:2001127$2DOI 100 1 $aLEONI, G. 245 $aSheep embryos derived from FSH/eCG treatment have a lower in vitro viability after vitrification than those derived from FSH treatment.$h[electronic resource] 260 $c2001 520 $aAbstract: In the non breeding period, the effect of two superovulatory treatments (eCG/FSH in single dose or FSH alone in four decreasing doses) on the production of embryo quality following in vitro viability after vitrification procedures was investigated using forty-four adult Sarda breed ewes. In sheep treated with eCG/FSH, the mean number of corpora lutea was significantly (P < 0.05) higher (11.8+/-4.0 vs. 8.05+/-3.8), although the recovery rate was significantly (P < 0.01) lower (74.6 vs. 59.9) than with FSH alone. After vitrification (ethylene glycol and glycerol) was repeated three times, the rates of re-expansion at first and second warming were significantly (P < 0.01) higher in embryos derived from FSH alone than in those with both gonadotrophins (94.9 and 41.9 vs. 72.8 and 18.6) and after the last vitrification the hatched blastocyst rates were 22.5 and 7.6. After differential stain, blastocysts derived from FSH alone showed a mean number of cells significantly higher than blastocysts from eCG/FSH (184.2 vs. 157.7). It was concluded that superovulatory treatment with eCG/FSH may increase the ovarian responses compared with FSH alone, but these embryos showed a reduction in viability rates after repeated vitrification. 650 $aCryopreservation 650 $aEmbryo transfer 650 $aEthylene 650 $aEthylene glycol 650 $aFollicle-stimulating hormone 650 $aGlycerol 650 $aPharmacology 650 $aPregnancy 650 $aReproduction 650 $aSheep 650 $aSuperovulation 650 $aVitrification 653 $aCryoprotective agents 653 $aDrug effects 653 $aeCG 653 $aEmbryo quality 653 $aFSH 653 $aIn Vitro Techniques 653 $aVitrificação 700 1 $aBOGLIOLO, L. 700 1 $aPINTUS, P. 700 1 $aLEDDA, S. 700 1 $aNAITANA, S. 773 $tReproduction, Nutrition, Development$gv. 41, n. 3, p. 239-246, May./Jun. 2001.
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1. |  | SCHWENGBER, J. E.; SCHIEDECK, G.; VIZZOTTO, M.; CAPELLESSO, A. J.; AUMONDE, T. Z.; SILVA, J. B.; MOREIRA, V.; PEREIRA, M. C. Produção orgânica e qualidade de cenouras semeadas segundo o calendário astronômico agrícola. Pelotas: Embrapa Clima Temperado, 2009. 25 p. (Embrapa Clima Temperado. Boletim de pesquisa e desenvolvimento, 91).Tipo: Boletim de Pesquisa e Desenvolvimento |
Biblioteca(s): Embrapa Clima Temperado. |
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2. |  | SCHWENGBER, J. E.; VIZZOTTO, M.; CAPELLESSO, A. J.; MOREIRA, V.; SCHIEDECK, G.; AUMONDE, T. Z.; SILVA, J. B. e.; PEREIRA, M. C. Produção orgânica e qualidade de cenouras semeadas segundo o calendário astrônomico agrícola. Agricultura Biodinâmica, 96, p.10-17, 2009.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Clima Temperado. |
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