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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
30/10/2000 |
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Data da última atualização: |
22/11/2002 |
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Autoria: |
PELLICER-RUBIO, M. T.; COMBARNOUS, Y. |
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Título: |
Deterioration of goat spermatozoa in skimmed milk-based extenders as a result of oleic acid released by the bulbourethral lipase BUSgp60. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Journal of Reproduction and Fertility, v. 112, n. 1, p. 95-105, 1998. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The aim of the present study was to elucidate the mode of action of goat bulbourethral lipase (BUSgp60 lipase) previously identified as responsible for the deterioration of goat sperm viability in skimmed milk-based extenders. Milk fractions were purified by micro- and ultrafiltration and characterized by SDS-PAGE, thin layer chromatography, triglyceride quantitative analysis and by their ability to potentiate the lipase and the sperm-deteriorating activity of the bulbourethral lipase. Components in both the phosphocaseinate and soluble whey protein fractions enhanced the lipase activity of BUSgp60 but only the phosphocaseinate fraction, which contains triglycerides, promoted deterioration of spermatozoa in the presence of bulbourethral gland secretion. These data suggest that the sperm-deteriorating effect of bulbourethral gland secretion is due to the catalysis of triglyceride hydrolysis, and that proteins increase this activity. BUSgp60 hydrolysed milk triglycerides and triolein very effectively, and its lipase activity was enhanced by several highly purified milk proteins. The major cis-unsaturated fatty acid from milk (oleic acid) but not the major saturated fatty acid (palmitic acid) exhibited dose-dependent detrimental effects on goat spermatozoa. Therefore, the catalysis of oleic acid formation from residual milk triglycerides by BUSgp60 appears responsible for the deterioration of goat spermatozoa when unwashed semen is diluted in skimmed milk-based extenders. The precise mechanism of action of oleic acid remains to be elucidated but the drawbacks of washing buck semen might be avoided by inhibiting BUSgp60 or by depriving it of substrate. MenosAbstract: The aim of the present study was to elucidate the mode of action of goat bulbourethral lipase (BUSgp60 lipase) previously identified as responsible for the deterioration of goat sperm viability in skimmed milk-based extenders. Milk fractions were purified by micro- and ultrafiltration and characterized by SDS-PAGE, thin layer chromatography, triglyceride quantitative analysis and by their ability to potentiate the lipase and the sperm-deteriorating activity of the bulbourethral lipase. Components in both the phosphocaseinate and soluble whey protein fractions enhanced the lipase activity of BUSgp60 but only the phosphocaseinate fraction, which contains triglycerides, promoted deterioration of spermatozoa in the presence of bulbourethral gland secretion. These data suggest that the sperm-deteriorating effect of bulbourethral gland secretion is due to the catalysis of triglyceride hydrolysis, and that proteins increase this activity. BUSgp60 hydrolysed milk triglycerides and triolein very effectively, and its lipase activity was enhanced by several highly purified milk proteins. The major cis-unsaturated fatty acid from milk (oleic acid) but not the major saturated fatty acid (palmitic acid) exhibited dose-dependent detrimental effects on goat spermatozoa. Therefore, the catalysis of oleic acid formation from residual milk triglycerides by BUSgp60 appears responsible for the deterioration of goat spermatozoa when unwashed semen is diluted in skimmed milk-based extend... Mostrar Tudo |
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Palavras-Chave: |
Bulburetral lipase; Cell Survival; Cromotografia; Drug effects; Plasma seminal; Semen Preservation. |
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Thesagro: |
Caprino; Espermatozóide; Lípase; Sêmen. |
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Thesaurus Nal: |
Analysis of variance; Dose response; Goats; Lipids; Milk proteins; Oleic acid; Pharmacology; Regression analysis; Spermatozoa. |
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Categoria do assunto: |
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L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02733naa a2200361 a 4500
001 1528872
005 2002-11-22
008 1998 bl uuuu u00u1 u #d
100 1 $aPELLICER-RUBIO, M. T.
245 $aDeterioration of goat spermatozoa in skimmed milk-based extenders as a result of oleic acid released by the bulbourethral lipase BUSgp60.$h[electronic resource]
260 $c1998
520 $aAbstract: The aim of the present study was to elucidate the mode of action of goat bulbourethral lipase (BUSgp60 lipase) previously identified as responsible for the deterioration of goat sperm viability in skimmed milk-based extenders. Milk fractions were purified by micro- and ultrafiltration and characterized by SDS-PAGE, thin layer chromatography, triglyceride quantitative analysis and by their ability to potentiate the lipase and the sperm-deteriorating activity of the bulbourethral lipase. Components in both the phosphocaseinate and soluble whey protein fractions enhanced the lipase activity of BUSgp60 but only the phosphocaseinate fraction, which contains triglycerides, promoted deterioration of spermatozoa in the presence of bulbourethral gland secretion. These data suggest that the sperm-deteriorating effect of bulbourethral gland secretion is due to the catalysis of triglyceride hydrolysis, and that proteins increase this activity. BUSgp60 hydrolysed milk triglycerides and triolein very effectively, and its lipase activity was enhanced by several highly purified milk proteins. The major cis-unsaturated fatty acid from milk (oleic acid) but not the major saturated fatty acid (palmitic acid) exhibited dose-dependent detrimental effects on goat spermatozoa. Therefore, the catalysis of oleic acid formation from residual milk triglycerides by BUSgp60 appears responsible for the deterioration of goat spermatozoa when unwashed semen is diluted in skimmed milk-based extenders. The precise mechanism of action of oleic acid remains to be elucidated but the drawbacks of washing buck semen might be avoided by inhibiting BUSgp60 or by depriving it of substrate.
650 $aAnalysis of variance
650 $aDose response
650 $aGoats
650 $aLipids
650 $aMilk proteins
650 $aOleic acid
650 $aPharmacology
650 $aRegression analysis
650 $aSpermatozoa
650 $aCaprino
650 $aEspermatozóide
650 $aLípase
650 $aSêmen
653 $aBulburetral lipase
653 $aCell Survival
653 $aCromotografia
653 $aDrug effects
653 $aPlasma seminal
653 $aSemen Preservation
700 1 $aCOMBARNOUS, Y.
773 $tJournal of Reproduction and Fertility$gv. 112, n. 1, p. 95-105, 1998.
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Embrapa Caprinos e Ovinos (CNPC) |
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| 1. |  | GOICOCHEA, S. L. K.; DEL SANTO, V. R.; BONANI, W. L.; SOUZA, M. H. DE; PIEROBON, V. A. DE O.; CAVALLARI, M. M. Caracterização morfológica de espiguetas de acessos do Banco Ativo de Germoplasma de Paspalum L. da Embrapa Pecuária Sudeste. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 14., 2022, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação; Embrapa Pecuária Sudeste, 2022. p. 71. (Embrapa Instrumentação. Documentos, 73).| Tipo: Resumo em Anais de Congresso |
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