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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
18/03/1996 |
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Data da última atualização: |
28/11/2023 |
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Autoria: |
DIAZ-APARICIO, E.; MARIN, C.; ALONSO-URMENETA, B.; ARAGON, V.; PEREZ-ORTIZ, S.; PARDO, M.; BLASCO, J. M.; DIAZ, R.; MORIYON, I. |
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Título: |
Evaluation of serological tests for diagnosis of Brucells melitensis infection of goats. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Journal of Clinical Microbiology, v. 32, n. 5, p. 1159-1165, May, 1994. |
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DOI: |
10.1128/jcm.32.5.1159-1165.1994 |
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Idioma: |
Inglês |
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Conteúdo: |
Five serological assays were evaluated for the diagnosis of brucellosis in goats: the rose bengal test (RBT), complement fixation test (CFT), radial immunodiffusion (RID) with Brucella and Yersinia enterocolitica O:9 polysaccharides, counterimmunoelectrophoresis (CIEP) with cytosol, and enzyme-linked immunosorbent assay (ELISA) with polyclonal and protein G conjugates and smooth lipopolysaccharide (S-LPS), native hapten polysaccharide (NH), or cytosol antigens. For optimal sensitivity, RBT had to be used with sera-antigen at a 3:1 dilution. In the RID test, Brucella melitensis biotype 1 NH could not be replaced by Brucella abortus biotype 1 or Y. enterocolitica 0:9 polysaccharides. In the ELISA, S-LPS and NH gave similar results and the protein G conjugate increased the specificity. With the sera from 55 B. melitensis culture-positive goats, the sensitivity was 100% for RBT, CFT (titer > or = 4), and ELISA with S-LPS or NH; 94% for RID; and 93% for CIEP. All tests were negative (100% specific) when testing the sera from 127 brucella-free goats. Larger discrepancies among the results of the serological tests were obtained with sera from goats of areas where brucellosis is endemic. When the sera of 20 young goats vaccinated subcutaneously (10(9) CFU of B. melitensis Rev 1) and bled 6 months later were examined, the specificities were as follows: NH ELISA, 60%; CFT and S-LPS ELISA, 75%; RBT, 80%; CIEP, 90%; and RID, 94%. With the sera from 10 young goats vaccinated conjunctivally (10(9) CFU of B. melitensis Rev 1) all tests were 100% specific 4 months after vaccination. The proportion of goats giving a positive reaction after vaccination decreased faster in RID than in other tests. MenosFive serological assays were evaluated for the diagnosis of brucellosis in goats: the rose bengal test (RBT), complement fixation test (CFT), radial immunodiffusion (RID) with Brucella and Yersinia enterocolitica O:9 polysaccharides, counterimmunoelectrophoresis (CIEP) with cytosol, and enzyme-linked immunosorbent assay (ELISA) with polyclonal and protein G conjugates and smooth lipopolysaccharide (S-LPS), native hapten polysaccharide (NH), or cytosol antigens. For optimal sensitivity, RBT had to be used with sera-antigen at a 3:1 dilution. In the RID test, Brucella melitensis biotype 1 NH could not be replaced by Brucella abortus biotype 1 or Y. enterocolitica 0:9 polysaccharides. In the ELISA, S-LPS and NH gave similar results and the protein G conjugate increased the specificity. With the sera from 55 B. melitensis culture-positive goats, the sensitivity was 100% for RBT, CFT (titer > or = 4), and ELISA with S-LPS or NH; 94% for RID; and 93% for CIEP. All tests were negative (100% specific) when testing the sera from 127 brucella-free goats. Larger discrepancies among the results of the serological tests were obtained with sera from goats of areas where brucellosis is endemic. When the sera of 20 young goats vaccinated subcutaneously (10(9) CFU of B. melitensis Rev 1) and bled 6 months later were examined, the specificities were as follows: NH ELISA, 60%; CFT and S-LPS ELISA, 75%; RBT, 80%; CIEP, 90%; and RID, 94%. With the sera from 10 young goats vaccinated conjunctival... Mostrar Tudo |
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Palavras-Chave: |
Dianostico; Sensitivity and specificity; Serologic Test methods. |
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Thesagro: |
Brucelose; Caprino; Doença. |
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Thesaurus Nal: |
Antigens; Brucellosis; Disease control; Disease diagnosis; Disease prevention; Goat diseases; Goats; Immunology; Pharmacology; Rose bengal; Vaccination. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02904naa a2200433 a 4500
001 1523385
005 2023-11-28
008 1994 bl uuuu u00u1 u #d
024 7 $a10.1128/jcm.32.5.1159-1165.1994$2DOI
100 1 $aDIAZ-APARICIO, E.
245 $aEvaluation of serological tests for diagnosis of Brucells melitensis infection of goats.$h[electronic resource]
260 $c1994
520 $aFive serological assays were evaluated for the diagnosis of brucellosis in goats: the rose bengal test (RBT), complement fixation test (CFT), radial immunodiffusion (RID) with Brucella and Yersinia enterocolitica O:9 polysaccharides, counterimmunoelectrophoresis (CIEP) with cytosol, and enzyme-linked immunosorbent assay (ELISA) with polyclonal and protein G conjugates and smooth lipopolysaccharide (S-LPS), native hapten polysaccharide (NH), or cytosol antigens. For optimal sensitivity, RBT had to be used with sera-antigen at a 3:1 dilution. In the RID test, Brucella melitensis biotype 1 NH could not be replaced by Brucella abortus biotype 1 or Y. enterocolitica 0:9 polysaccharides. In the ELISA, S-LPS and NH gave similar results and the protein G conjugate increased the specificity. With the sera from 55 B. melitensis culture-positive goats, the sensitivity was 100% for RBT, CFT (titer > or = 4), and ELISA with S-LPS or NH; 94% for RID; and 93% for CIEP. All tests were negative (100% specific) when testing the sera from 127 brucella-free goats. Larger discrepancies among the results of the serological tests were obtained with sera from goats of areas where brucellosis is endemic. When the sera of 20 young goats vaccinated subcutaneously (10(9) CFU of B. melitensis Rev 1) and bled 6 months later were examined, the specificities were as follows: NH ELISA, 60%; CFT and S-LPS ELISA, 75%; RBT, 80%; CIEP, 90%; and RID, 94%. With the sera from 10 young goats vaccinated conjunctivally (10(9) CFU of B. melitensis Rev 1) all tests were 100% specific 4 months after vaccination. The proportion of goats giving a positive reaction after vaccination decreased faster in RID than in other tests.
650 $aAntigens
650 $aBrucellosis
650 $aDisease control
650 $aDisease diagnosis
650 $aDisease prevention
650 $aGoat diseases
650 $aGoats
650 $aImmunology
650 $aPharmacology
650 $aRose bengal
650 $aVaccination
650 $aBrucelose
650 $aCaprino
650 $aDoença
653 $aDianostico
653 $aSensitivity and specificity
653 $aSerologic Test methods
700 1 $aMARIN, C.
700 1 $aALONSO-URMENETA, B.
700 1 $aARAGON, V.
700 1 $aPEREZ-ORTIZ, S.
700 1 $aPARDO, M.
700 1 $aBLASCO, J. M.
700 1 $aDIAZ, R.
700 1 $aMORIYON, I.
773 $tJournal of Clinical Microbiology$gv. 32, n. 5, p. 1159-1165, May, 1994.
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Embrapa Caprinos e Ovinos (CNPC) |
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