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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
18/03/1996 |
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Data da última atualização: |
28/11/2023 |
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Autoria: |
DIAZ-APARICIO, E.; MARIN, C.; ALONSO-URMENETA, B.; ARAGON, V.; PEREZ-ORTIZ, S.; PARDO, M.; BLASCO, J. M.; DIAZ, R.; MORIYON, I. |
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Título: |
Evaluation of serological tests for diagnosis of Brucells melitensis infection of goats. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Journal of Clinical Microbiology, v. 32, n. 5, p. 1159-1165, May, 1994. |
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DOI: |
10.1128/jcm.32.5.1159-1165.1994 |
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Idioma: |
Inglês |
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Conteúdo: |
Five serological assays were evaluated for the diagnosis of brucellosis in goats: the rose bengal test (RBT), complement fixation test (CFT), radial immunodiffusion (RID) with Brucella and Yersinia enterocolitica O:9 polysaccharides, counterimmunoelectrophoresis (CIEP) with cytosol, and enzyme-linked immunosorbent assay (ELISA) with polyclonal and protein G conjugates and smooth lipopolysaccharide (S-LPS), native hapten polysaccharide (NH), or cytosol antigens. For optimal sensitivity, RBT had to be used with sera-antigen at a 3:1 dilution. In the RID test, Brucella melitensis biotype 1 NH could not be replaced by Brucella abortus biotype 1 or Y. enterocolitica 0:9 polysaccharides. In the ELISA, S-LPS and NH gave similar results and the protein G conjugate increased the specificity. With the sera from 55 B. melitensis culture-positive goats, the sensitivity was 100% for RBT, CFT (titer > or = 4), and ELISA with S-LPS or NH; 94% for RID; and 93% for CIEP. All tests were negative (100% specific) when testing the sera from 127 brucella-free goats. Larger discrepancies among the results of the serological tests were obtained with sera from goats of areas where brucellosis is endemic. When the sera of 20 young goats vaccinated subcutaneously (10(9) CFU of B. melitensis Rev 1) and bled 6 months later were examined, the specificities were as follows: NH ELISA, 60%; CFT and S-LPS ELISA, 75%; RBT, 80%; CIEP, 90%; and RID, 94%. With the sera from 10 young goats vaccinated conjunctivally (10(9) CFU of B. melitensis Rev 1) all tests were 100% specific 4 months after vaccination. The proportion of goats giving a positive reaction after vaccination decreased faster in RID than in other tests. MenosFive serological assays were evaluated for the diagnosis of brucellosis in goats: the rose bengal test (RBT), complement fixation test (CFT), radial immunodiffusion (RID) with Brucella and Yersinia enterocolitica O:9 polysaccharides, counterimmunoelectrophoresis (CIEP) with cytosol, and enzyme-linked immunosorbent assay (ELISA) with polyclonal and protein G conjugates and smooth lipopolysaccharide (S-LPS), native hapten polysaccharide (NH), or cytosol antigens. For optimal sensitivity, RBT had to be used with sera-antigen at a 3:1 dilution. In the RID test, Brucella melitensis biotype 1 NH could not be replaced by Brucella abortus biotype 1 or Y. enterocolitica 0:9 polysaccharides. In the ELISA, S-LPS and NH gave similar results and the protein G conjugate increased the specificity. With the sera from 55 B. melitensis culture-positive goats, the sensitivity was 100% for RBT, CFT (titer > or = 4), and ELISA with S-LPS or NH; 94% for RID; and 93% for CIEP. All tests were negative (100% specific) when testing the sera from 127 brucella-free goats. Larger discrepancies among the results of the serological tests were obtained with sera from goats of areas where brucellosis is endemic. When the sera of 20 young goats vaccinated subcutaneously (10(9) CFU of B. melitensis Rev 1) and bled 6 months later were examined, the specificities were as follows: NH ELISA, 60%; CFT and S-LPS ELISA, 75%; RBT, 80%; CIEP, 90%; and RID, 94%. With the sera from 10 young goats vaccinated conjunctival... Mostrar Tudo |
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Palavras-Chave: |
Dianostico; Sensitivity and specificity; Serologic Test methods. |
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Thesagro: |
Brucelose; Caprino; Doença. |
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Thesaurus Nal: |
Antigens; Brucellosis; Disease control; Disease diagnosis; Disease prevention; Goat diseases; Goats; Immunology; Pharmacology; Rose bengal; Vaccination. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02904naa a2200433 a 4500
001 1523385
005 2023-11-28
008 1994 bl uuuu u00u1 u #d
024 7 $a10.1128/jcm.32.5.1159-1165.1994$2DOI
100 1 $aDIAZ-APARICIO, E.
245 $aEvaluation of serological tests for diagnosis of Brucells melitensis infection of goats.$h[electronic resource]
260 $c1994
520 $aFive serological assays were evaluated for the diagnosis of brucellosis in goats: the rose bengal test (RBT), complement fixation test (CFT), radial immunodiffusion (RID) with Brucella and Yersinia enterocolitica O:9 polysaccharides, counterimmunoelectrophoresis (CIEP) with cytosol, and enzyme-linked immunosorbent assay (ELISA) with polyclonal and protein G conjugates and smooth lipopolysaccharide (S-LPS), native hapten polysaccharide (NH), or cytosol antigens. For optimal sensitivity, RBT had to be used with sera-antigen at a 3:1 dilution. In the RID test, Brucella melitensis biotype 1 NH could not be replaced by Brucella abortus biotype 1 or Y. enterocolitica 0:9 polysaccharides. In the ELISA, S-LPS and NH gave similar results and the protein G conjugate increased the specificity. With the sera from 55 B. melitensis culture-positive goats, the sensitivity was 100% for RBT, CFT (titer > or = 4), and ELISA with S-LPS or NH; 94% for RID; and 93% for CIEP. All tests were negative (100% specific) when testing the sera from 127 brucella-free goats. Larger discrepancies among the results of the serological tests were obtained with sera from goats of areas where brucellosis is endemic. When the sera of 20 young goats vaccinated subcutaneously (10(9) CFU of B. melitensis Rev 1) and bled 6 months later were examined, the specificities were as follows: NH ELISA, 60%; CFT and S-LPS ELISA, 75%; RBT, 80%; CIEP, 90%; and RID, 94%. With the sera from 10 young goats vaccinated conjunctivally (10(9) CFU of B. melitensis Rev 1) all tests were 100% specific 4 months after vaccination. The proportion of goats giving a positive reaction after vaccination decreased faster in RID than in other tests.
650 $aAntigens
650 $aBrucellosis
650 $aDisease control
650 $aDisease diagnosis
650 $aDisease prevention
650 $aGoat diseases
650 $aGoats
650 $aImmunology
650 $aPharmacology
650 $aRose bengal
650 $aVaccination
650 $aBrucelose
650 $aCaprino
650 $aDoença
653 $aDianostico
653 $aSensitivity and specificity
653 $aSerologic Test methods
700 1 $aMARIN, C.
700 1 $aALONSO-URMENETA, B.
700 1 $aARAGON, V.
700 1 $aPEREZ-ORTIZ, S.
700 1 $aPARDO, M.
700 1 $aBLASCO, J. M.
700 1 $aDIAZ, R.
700 1 $aMORIYON, I.
773 $tJournal of Clinical Microbiology$gv. 32, n. 5, p. 1159-1165, May, 1994.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 17 | |
| 2. |  | VEIGA, J. B. da; QUANZ, D.; CRUZ, E. D. Avaliação de forrageiras em estabelecimentos rurais de Uruará-PA, na fronteira agrícola da Amazônia. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 34., 1997, Juiz de Fora, MG. Anais. Juiz de Fora: SBZ, 1997. v. 2, p. 24-26.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 6. | | PESSOA, R. O.; QUANZ, D.; LUDOVINO, R. R.; VEIGA, J. B. da; TOURRAND, J.-F. A atividade pecuária nos sistema de produção dos pequenos agricultores do baixo Amazonas. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 34., 1997, Juiz de Fora, MG. Anais. Juiz de Fora: SBZ, 1997. v. 4, p. 305-307.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 9. | | TOURRAND, J.-F.; VEIGA, J. B. da; QUANZ, D.; FERREIRA, L. A.; SIMAO NETO, M. Produção leiteira em área de fronteira agrícola da Amazônia: o caso do município de Uruará (PA), na Transamazônica. In: HOMMA, A. K. O. (Ed.). Amazônia: meio ambiente e desenvolvimento agrícola. Brasília, DF: EMBRAPA-SPI; Belém, PA: EMBRAPA-CPATU, 1998. p. 345-365.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 10. | | CELESTINO FILHO, P.; QUANZ, D.; BARBOSA, T. M. F.; NAHUM, B. de S. Roça permanente de lavoura branca (RPLB) na transamazônica: alternativas para a produção de culturas alimentares em áreas alteradas. Revista Amazônia, Belém, PA, ano 4, n. 14, p. 30-31, 2009.| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 11. | | HOMEM, V. S. F.; HEINEMANN, M. B.; MORAES, Z. M.; VEIGA, J. B. da; LAU, H. D.; QUANZ, D.; TOURRAND, J. F.; FERREIRA, F.; FERREIRA NETO, J. S. Leptospirose bovina e humana em Uruará, PA, município da Amazônia oriental. In: CONGRESSO BRASILEIRO DE MEDICINA VETERINÁRIA, 26., 1999, Campo Grande. Resumo dos trabalhos científicos. Campo Grande, MS: SBMV: SOMVET: CRMV, 1999. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 12. | | CELESTINO FILHO, P.; QUANZ, D.; BARBOSA, T. M. F.; SCOPEL, E. J.; VEIGA, J. B. da; SIST, P. L. J.; TOURRAND, J.-F.; NAHUM, B. de S. Plantio direto em áreas alteradas na agricultura familiar. Belém, PA: Embrapa Amazônia Oriental: FLOAGRI: CIRAD, 2009. 23 p. il. Cartilha.| Tipo: Folder/Folheto/Cartilha |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 13. | | HOMEM, V. S. F.; HEINEMANN, M. B.; MORAES, Z. M.; VEIGA, J. B. da; LAU, H. D.; QUANZ, D.; TOURRAND, J. F.; FERREIRA, F.; FERREIRA NETO, J. S. Brucelose e tuberculose em Uruará, PA, município da Amazônia oriental. Estudo da população bovina e humana In: CONGRESSO BRASILEIRO DE MEDICINA VETERINÁRIA, 26., 1999, Campo Grande. Resumo dos trabalhos científicos. Campo Grande, MS: SBMV: SOMVET: CRMV, 1999. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 14. | | TOURRAND, J.-F.; FERREIRA, L. A.; VEIGA, J. B. da; QUANZ, D.; LUDOVINO, R. R.; LAU, H. D.; VIEIRA, L. C. A produção leiteira na fronteira agrícola da Amazônia Oriental brasileira: situação atual e perspectivas. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 34., 1997, Juiz de Fora, MG. Anais. Juiz de Fora: SBZ, 1997. v. 4, p. 314-316.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 15. | | BARBOSA, T. M. F.; TOURRAND, J.-F.; NAHUM, B. de S.; VEIGA, J. B. da; QUANZ, D.; SIST, P.; SCOPEL, E. The start of sustainability in Amazonia? A new paradigm based on fertility for sustainable land management. In: EUROPEAN IFSA SYMPOSIUM, 8., 2008, Clermont Ferrand. Empowerment of the rural actors: a renewal of farming systems perspectives. [S.l.]: IFSA, 2008. p. 591-600.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 16. | | BARBOSA, T. M. F.; TOURRAND, J. F.; NAHUM, B. S.; VEIGA, J. B. da; QUANZ, D.; SIST, P.; SCOPEL, E.; HOMEM, V. S. F. Well-being, work comfort and food security are better than maximizing production in the Amazon. In: WORLD CONGRESS OF RURAL SOCIOLOGY, 12., 2008, Goyang. Envisioning prosperous rural futures in a globalizing world. [S.l.]: IRSA, 2008.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 17. | | HOMEM, V. S. F.; HEINEMANN, M. B.; MORAES, Z. M.; VIANNA, M. C. B.; SILVA, J. C. R.; SAKAMOTO, S. M.; PINHEIRO, S. R.; VEIGA, J. B. da; LAU, H. D.; QUANZ, D.; TOURRAND, J.-F.; FERREIRA, F.; FERREIRA NETO, J. S. Leptospirose bovina em Uruará, PA, município da Amazônia Oriental. Arquivos do Instituto Biológico, v. 67, n. 1, p. 1-10, 2000.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 17 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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