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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
26/02/1996 |
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Data da última atualização: |
23/06/2023 |
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Autoria: |
ELLIS, J. A.; HAWK, D. A.; MILLS, K. W.; PRATT, D. L. |
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Título: |
Antigen specificity of antibody responses to Corynebacterium pseudotuberculosis in naturally infected sheep with caseous lymphadenitis. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Veterinary Immunology and Immunopathology, v. 28, n. 3/4, p. 289-301, Jul. 1991. |
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DOI: |
10.1016/0165-2427(91)90121-r. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Constituents of Corynebacterium pseudotuberculosis were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Analysis of sonicated whole bacterial cells and ether-extracted cells revealed more than 35 bands in silver-stained gels. SDS-PAGE analysis of concentrated culture filtrates with exotoxin activity demonstrated more than 15 bands. Sera from sheep with C. pseudotuberculosis-induced disease of variable severity were used to probe immunoblots of electrophoresed ether-extracted cells and culture filtrates. Twenty or more corynebacterial molecules, ranging in molecular weight from 20 to 112 kDa, in ether-extracted cells were recognized by antibodies in the sera of naturally exposed sheep with positive ELISA titers. These sera also recognized up to six molecules, ranging from, 20 to 68.1 kDa, on immunoblots of ammonium sulfate-concentrated culture filtrate. There was no apparent relationship between the stage of disease and the response to specific corynebacterial antigens in these animals. |
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Thesagro: |
Corynebacterium Pseudotuberculosis; Doença; Linfadenite Caseosa; Ovino. |
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Thesaurus Nal: |
Bacterial antibodies; Biosynthesis; Caseous lymphadenitis; Enzyme-linked immunosorbent assay; Immunodominant epitopes; Immunology; Lymphadenitis; Polyacrylamide gel electrophoresis; Sheep diseases. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02109naa a2200325 a 4500
001 1523338
005 2023-06-23
008 1991 bl uuuu u00u1 u #d
024 7 $a10.1016/0165-2427(91)90121-r.$2DOI
100 1 $aELLIS, J. A.
245 $aAntigen specificity of antibody responses to Corynebacterium pseudotuberculosis in naturally infected sheep with caseous lymphadenitis.$h[electronic resource]
260 $c1991
520 $aAbstract: Constituents of Corynebacterium pseudotuberculosis were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Analysis of sonicated whole bacterial cells and ether-extracted cells revealed more than 35 bands in silver-stained gels. SDS-PAGE analysis of concentrated culture filtrates with exotoxin activity demonstrated more than 15 bands. Sera from sheep with C. pseudotuberculosis-induced disease of variable severity were used to probe immunoblots of electrophoresed ether-extracted cells and culture filtrates. Twenty or more corynebacterial molecules, ranging in molecular weight from 20 to 112 kDa, in ether-extracted cells were recognized by antibodies in the sera of naturally exposed sheep with positive ELISA titers. These sera also recognized up to six molecules, ranging from, 20 to 68.1 kDa, on immunoblots of ammonium sulfate-concentrated culture filtrate. There was no apparent relationship between the stage of disease and the response to specific corynebacterial antigens in these animals.
650 $aBacterial antibodies
650 $aBiosynthesis
650 $aCaseous lymphadenitis
650 $aEnzyme-linked immunosorbent assay
650 $aImmunodominant epitopes
650 $aImmunology
650 $aLymphadenitis
650 $aPolyacrylamide gel electrophoresis
650 $aSheep diseases
650 $aCorynebacterium Pseudotuberculosis
650 $aDoença
650 $aLinfadenite Caseosa
650 $aOvino
700 1 $aHAWK, D. A.
700 1 $aMILLS, K. W.
700 1 $aPRATT, D. L.
773 $tVeterinary Immunology and Immunopathology$gv. 28, n. 3/4, p. 289-301, Jul. 1991.
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