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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
02/01/2024 |
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Autoria: |
EMIK, L. O.; SIDWELL, G. M. |
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Título: |
Refining methods for using opal blue stain in evaluating ram semen. |
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Ano de publicação: |
1947 |
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Fonte/Imprenta: |
Journal of Animal Science, v. 6, n. 1, p. 67-71, Feb. 1947. |
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DOI: |
https://doi.org/10.2527/jas1947.6167 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: For ram's semen the optimum stain mixture for opal blue Breslau and eosin was found to be: 12.5 percent opal blue and 0.5 percent eosin in pH 7.15 phosphate buffer. It was necessary to stain for 30 seconds before smearing. The dry smears were most suitable for observation. Balsam or oil decreases the color contrast. No suitable method was found to incorporate opal blue and eosin into a diluting fluid for use in the haemocytometer. Under normal conditions of examination motile sperm took no stain, and unstained sperm were therefore regarded as the best estimate of the live sperm. Prediction of percent live normal spermatozoa (on smear) from a combination (EMC) of the motility score and estimated percent motile had significant reliability. The opal blue staining method was found to be simpler and more rapid than previous methods, allowing immediate examination for abnormalities of the sperm during routine examination of semen. The slides and the stain solution exhibited no noticeable depreciation after four years. |
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Palavras-Chave: |
Andrologia; Labeling. |
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Thesagro: |
Ovino; Reprodução; Sêmen. |
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Thesaurus Nal: |
Congenital abnormalities; Sheep; Spermatozoa; Staining. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01739naa a2200253 a 4500
001 1523053
005 2024-01-02
008 1947 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.2527/jas1947.6167$2DOI
100 1 $aEMIK, L. O.
245 $aRefining methods for using opal blue stain in evaluating ram semen.$h[electronic resource]
260 $c1947
520 $aAbstract: For ram's semen the optimum stain mixture for opal blue Breslau and eosin was found to be: 12.5 percent opal blue and 0.5 percent eosin in pH 7.15 phosphate buffer. It was necessary to stain for 30 seconds before smearing. The dry smears were most suitable for observation. Balsam or oil decreases the color contrast. No suitable method was found to incorporate opal blue and eosin into a diluting fluid for use in the haemocytometer. Under normal conditions of examination motile sperm took no stain, and unstained sperm were therefore regarded as the best estimate of the live sperm. Prediction of percent live normal spermatozoa (on smear) from a combination (EMC) of the motility score and estimated percent motile had significant reliability. The opal blue staining method was found to be simpler and more rapid than previous methods, allowing immediate examination for abnormalities of the sperm during routine examination of semen. The slides and the stain solution exhibited no noticeable depreciation after four years.
650 $aCongenital abnormalities
650 $aSheep
650 $aSpermatozoa
650 $aStaining
650 $aOvino
650 $aReprodução
650 $aSêmen
653 $aAndrologia
653 $aLabeling
700 1 $aSIDWELL, G. M.
773 $tJournal of Animal Science$gv. 6, n. 1, p. 67-71, Feb. 1947.
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| Registros recuperados : 3 | |
| 2. |  | MOCHIUTTI, S.; CAPORAL, F. J. M.; GUGLIERI, A.; HIGA, A. R.; BEHLING, M. Comportamento de forrageiras nativas em sistemas silvipastoris com acácia-negra no Rio Grande do Sul. In: CONGRESSO BRASILEIRO DE SISTEMAS AGROFLORESTAIS, 7., 2009, Luziânia. Diálogo e integração de saberes em sistemas agroflorestais para sociedades sustentáveis: anais. [Luziânia]: Sociedade Brasileira de Sistemas Agroflorestais; [Brasília, DF]: EMATER-DF: Embrapa, 2009. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amapá. |
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| 3. | | GOMES, K. E.; OLIVEIRA, J. C. P.; DUTRA, G. M.; GUGLIERI, A.; BENAVIDES, M. V. Coleta de germoplasma de Bromus auleticus nos estados do Rio Grande do Sul e Santa Catarina - Brasil. In: REUNIÃO TEMÁTICA INTERNACIONAL SOBRE O GÊNERO BROMUS, 2000, Bagé. Resultado da pesquisa em recursos genéticos sobre o genero Bromus: resumos. Bagé: Embrapa Pecuária Sul, 2001. p. 28. (Embrapa Pecuária Sul. Documentos, 36). Organizadores: Klecius Ellera Gomes, José Otávio Neto Gonçalves, João Carlos Pinto Oliveira.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| Registros recuperados : 3 | |
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