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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
30/06/2023 |
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Autoria: |
LINZELL, J. L.; FLEET, I. R.; MEPHAM, T. B.; PEAKER, M. |
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Título: |
Perfusion of the isolated mammary gland of the goat. |
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Ano de publicação: |
1972 |
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Fonte/Imprenta: |
Quarterly Journal of Experimental Physiology, v. 57, p. 139-161, 1972. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Improvements to a previously reported technique for the perfusion of isolated goat mammary glands are described. Secretion of milk of relatively normal composition in vitro has been maintained at approximately 50% of the in vivo rate for about 12 hr. Factors found to be important for successful perfusion are a normal rate of mammary blood flow, the provision of adequate glucose, acetate and amino acids, and the inclusion in the circuit of an efficient oxygenator and dialyser. Contrary to earlier results successful perfusions have been performed with glands from anaesthetized and from shot animals, although removal of glands from the tranquillized animal under spinal anaesthesia is still preferred. There was a marked dependence of milk secretion rate on the rate of blood flow below a critical value, but at supranormal flows there was no correlation between these variables. Evidence has been obtained suggesting that kinins or similar substances may be formed during perfusion. The specific dependence of milk secretion on glucose is again stressed. Other carbohydrates sustained oxygen consumption similar to that shown with glucose, but by contrast did not support the secretion of water, a small amount of milk low in lactose but high in fat and protein being produced. Although milk fat secretion was dependent on the provision of acetate, the omission of fl-hydroxybutyrate, lactate and neutral fat (all shown to be absorbed by the gland in vivo) from the substrate mixture did not seriously impair milk fat secretion in vitro. Amino acids were shown to be necessary for normal rates of milk protein secretion, but the alteration of the substrate mixture to correspond to the amino acid absorption observed in vivo produced no significant change in milk secretion from that observed when amino acids were infused at rates corresponding to their occurrence in casein. The infusion of B vitamins and various hormones had no effect on milk secretion. Perfusions carried out with a conscious goat in circuit were no more successful than the completely in vitro system, but glands grafted onto recipient goats (even males) following complete removal from the donors produced substantially more milk than in vitro preparations. In some experiments milk [Na] and [Cl] increased during perfusion while [K] and [lactose] decreased, these trends being apparent in milk secreted before perfusion was begun and being probably due to the oxytocin used to produce milk ejection. In other experiments milk [Na] and [Cl] showed little change but [K] and [lactose] tended to rise. Possible factors limiting the performance of perfused organs are discussed. MenosAbstract: Improvements to a previously reported technique for the perfusion of isolated goat mammary glands are described. Secretion of milk of relatively normal composition in vitro has been maintained at approximately 50% of the in vivo rate for about 12 hr. Factors found to be important for successful perfusion are a normal rate of mammary blood flow, the provision of adequate glucose, acetate and amino acids, and the inclusion in the circuit of an efficient oxygenator and dialyser. Contrary to earlier results successful perfusions have been performed with glands from anaesthetized and from shot animals, although removal of glands from the tranquillized animal under spinal anaesthesia is still preferred. There was a marked dependence of milk secretion rate on the rate of blood flow below a critical value, but at supranormal flows there was no correlation between these variables. Evidence has been obtained suggesting that kinins or similar substances may be formed during perfusion. The specific dependence of milk secretion on glucose is again stressed. Other carbohydrates sustained oxygen consumption similar to that shown with glucose, but by contrast did not support the secretion of water, a small amount of milk low in lactose but high in fat and protein being produced. Although milk fat secretion was dependent on the provision of acetate, the omission of fl-hydroxybutyrate, lactate and neutral fat (all shown to be absorbed by the gland in vivo) from the substrate mixture... Mostrar Tudo |
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Palavras-Chave: |
In Vitro Techniques. |
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Thesagro: |
Caprino; Fisiologia; Glândula Mamaria; Lactose; Reprodução. |
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Thesaurus Nal: |
Amino acids; Bradykinin; Goats; Lactation; Lymph; Mammary glands; Mannose; Oxygen consumption; Pharmacology; Potassium; Reproduction. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03594naa a2200361 a 4500
001 1522698
005 2023-06-30
008 1972 bl uuuu u00u1 u #d
100 1 $aLINZELL, J. L.
245 $aPerfusion of the isolated mammary gland of the goat.$h[electronic resource]
260 $c1972
520 $aAbstract: Improvements to a previously reported technique for the perfusion of isolated goat mammary glands are described. Secretion of milk of relatively normal composition in vitro has been maintained at approximately 50% of the in vivo rate for about 12 hr. Factors found to be important for successful perfusion are a normal rate of mammary blood flow, the provision of adequate glucose, acetate and amino acids, and the inclusion in the circuit of an efficient oxygenator and dialyser. Contrary to earlier results successful perfusions have been performed with glands from anaesthetized and from shot animals, although removal of glands from the tranquillized animal under spinal anaesthesia is still preferred. There was a marked dependence of milk secretion rate on the rate of blood flow below a critical value, but at supranormal flows there was no correlation between these variables. Evidence has been obtained suggesting that kinins or similar substances may be formed during perfusion. The specific dependence of milk secretion on glucose is again stressed. Other carbohydrates sustained oxygen consumption similar to that shown with glucose, but by contrast did not support the secretion of water, a small amount of milk low in lactose but high in fat and protein being produced. Although milk fat secretion was dependent on the provision of acetate, the omission of fl-hydroxybutyrate, lactate and neutral fat (all shown to be absorbed by the gland in vivo) from the substrate mixture did not seriously impair milk fat secretion in vitro. Amino acids were shown to be necessary for normal rates of milk protein secretion, but the alteration of the substrate mixture to correspond to the amino acid absorption observed in vivo produced no significant change in milk secretion from that observed when amino acids were infused at rates corresponding to their occurrence in casein. The infusion of B vitamins and various hormones had no effect on milk secretion. Perfusions carried out with a conscious goat in circuit were no more successful than the completely in vitro system, but glands grafted onto recipient goats (even males) following complete removal from the donors produced substantially more milk than in vitro preparations. In some experiments milk [Na] and [Cl] increased during perfusion while [K] and [lactose] decreased, these trends being apparent in milk secreted before perfusion was begun and being probably due to the oxytocin used to produce milk ejection. In other experiments milk [Na] and [Cl] showed little change but [K] and [lactose] tended to rise. Possible factors limiting the performance of perfused organs are discussed.
650 $aAmino acids
650 $aBradykinin
650 $aGoats
650 $aLactation
650 $aLymph
650 $aMammary glands
650 $aMannose
650 $aOxygen consumption
650 $aPharmacology
650 $aPotassium
650 $aReproduction
650 $aCaprino
650 $aFisiologia
650 $aGlândula Mamaria
650 $aLactose
650 $aReprodução
653 $aIn Vitro Techniques
700 1 $aFLEET, I. R.
700 1 $aMEPHAM, T. B.
700 1 $aPEAKER, M.
773 $tQuarterly Journal of Experimental Physiology$gv. 57, p. 139-161, 1972.
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| 1. |  | FERREIRA, I. C.; SILVA, P. M. R. S.; MALAQUIAS, J. V.; FONSECA NETO, A. M. da; OLIVEIRA, S. A. S.; PINHO, G. A. S.; MARTINS, C. F. Habituação de novilhas Zebuínas no pré-parto para entrar na sala de ordenha: avaliação qualitativa. Planaltina, DF : Embrapa Cerrados, 2020. 22 p. (Embrapa Cerrados. Boletim de Pesquisa e Desenvolvimento, 365).| Tipo: Boletim de Pesquisa e Desenvolvimento |
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| 3. | | FONSECA NETO, A. M. da; MARTINS, C. F.; MIZIARA, F.; PACHECO, G. R.; CUMPA, H. C. B.; FERREIRA, I. C.; BORGES, J. R. J.; BALBINO, L. C.; TOLETO, M. R. de; SILVA, P. M. R. S. da; GODOY, S. D. Terceira prova brasileira de produção de leite a pasto do Zebu Leiteiro no Centro de Tecnologias para Raças Zebuínas Leiteiras (CTZL) da Embrapa Cerrados: caderno de resultados para novilhas da raça Gir Leiteiro. Planaltina, DF: Embrapa Cerrados, 2019. 41 p. (Embrapa Cerrados. Documentos, 351).| Biblioteca(s): Embrapa Cerrados. |
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| Registros recuperados : 3 | |
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