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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
28/02/1996 |
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Data da última atualização: |
02/01/2024 |
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Autoria: |
DE SMEDT, V.; CROZET, N.; AHMED-ALI, M.; MARTINO, A.; COGNIE, Y. |
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Título: |
In vitro maturation and fertilization of goat oocytes. |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
Theriogenology, v.37, n.5, p.1049-1060, 1992. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Follicular cumulus-enclosed goat oocytes were matured in vitro in the presence of granulosa ceils, follicle stimulating hormone (FSH), luteinizing hormone (LH) and estradiol-178. While 86% of the oocytes from follicles 2 to 6 mm in diameter achieved meiotic maturation, only 24% of the oocytes from follicles 1 to 2 mm in diameter progressed to Metaphase II. Exposure of follicle-enclosed cumulus-oocyte complexes to 20°C prior to culture resulted in 11.5% of the oocytes exhibiting abnormal meiotic spindle. This indicated that immature goat oocytes are particularly sensitive to temperature. |
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Palavras-Chave: |
Cultura; Fertilizacao in vitro; Maturation. |
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Thesagro: |
Caprino. |
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Thesaurus Nal: |
Goats; In vitro fertilization; Oocytes; Reproduction. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01288naa a2200265 a 4500
001 1522472
005 2024-01-02
008 1992 bl uuuu u00u1 u #d
100 1 $aDE SMEDT, V.
245 $aIn vitro maturation and fertilization of goat oocytes.$h[electronic resource]
260 $c1992
520 $aAbstract: Follicular cumulus-enclosed goat oocytes were matured in vitro in the presence of granulosa ceils, follicle stimulating hormone (FSH), luteinizing hormone (LH) and estradiol-178. While 86% of the oocytes from follicles 2 to 6 mm in diameter achieved meiotic maturation, only 24% of the oocytes from follicles 1 to 2 mm in diameter progressed to Metaphase II. Exposure of follicle-enclosed cumulus-oocyte complexes to 20°C prior to culture resulted in 11.5% of the oocytes exhibiting abnormal meiotic spindle. This indicated that immature goat oocytes are particularly sensitive to temperature.
650 $aGoats
650 $aIn vitro fertilization
650 $aOocytes
650 $aReproduction
650 $aCaprino
653 $aCultura
653 $aFertilizacao in vitro
653 $aMaturation
700 1 $aCROZET, N.
700 1 $aAHMED-ALI, M.
700 1 $aMARTINO, A.
700 1 $aCOGNIE, Y.
773 $tTheriogenology$gv.37, n.5, p.1049-1060, 1992.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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