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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
29/03/2023 |
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Autoria: |
SEAMARK, R. F.; AMATO, F.; HENDRICKSON, S.; MOOR, R. M. |
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Afiliação: |
University of Adelaide, A.R.C. Unit of Reproductive Physiology and Biochemistry, Cambridge. |
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Título: |
Oxygen uptake, glucose utilization, lactate release and adenine nucleotide content of sheep pvarian follicles in culture: effect of human chorionic gonadotrophin. |
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Ano de publicação: |
1976 |
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Fonte/Imprenta: |
Australian Journal Biology Science, v. 29, n. 5/6, p. 557-563, Dec. 1976. |
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DOI: |
10.1071/bi9760557. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: A study has been made of the oxygen uptake, glucose utilization, lactate release and cellular content of adenine nucleotides of isolated sheep ovarian follicles (4-6 min in diameter) maintained in organ culture, and of the effects on these parameters of the addition of human chorionic gonadotrophin (hCG). The mean oxygen consumption of the entire follicles when freshly isolated and of the theca and membrana components was 0-56, 1-08 and 0-05 mumol per milligram wet weight of tissue per hour respectively. About 8 mumol of glucose was taken up and 16 mumol of lactate released per milligram wet weight of follicular tissue per hour during the first 24-h period of culture. This rate reduced by about 30% for each subsequent day of culture, but was significantly increased by the addition of hCG. The mean ATP content of theca and granulosa tissues was 4-6 and 2-8 nmol/mg wet weight of tissue respectively. There was no discernable change in tissue adenine nucleotide content or energy charge ratio during the 3-day culture period, and prolonged exposure to hCG was without effect. Untreated follicles produced both oestrogen and androgens throughout the culture period. The addition of hCG resulted in a transitory stimulation in oestrogen secretion, inhibition of androgen secretion, and a marked and sustained rise in progestin secretion. It is proposed that the increase in glycolytic activity following exposure to hCG may relate to the activation of the granulosa cells coincident with a transference of steroid synthetic capacity from theca interna to membrana granulosa. MenosAbstract: A study has been made of the oxygen uptake, glucose utilization, lactate release and cellular content of adenine nucleotides of isolated sheep ovarian follicles (4-6 min in diameter) maintained in organ culture, and of the effects on these parameters of the addition of human chorionic gonadotrophin (hCG). The mean oxygen consumption of the entire follicles when freshly isolated and of the theca and membrana components was 0-56, 1-08 and 0-05 mumol per milligram wet weight of tissue per hour respectively. About 8 mumol of glucose was taken up and 16 mumol of lactate released per milligram wet weight of follicular tissue per hour during the first 24-h period of culture. This rate reduced by about 30% for each subsequent day of culture, but was significantly increased by the addition of hCG. The mean ATP content of theca and granulosa tissues was 4-6 and 2-8 nmol/mg wet weight of tissue respectively. There was no discernable change in tissue adenine nucleotide content or energy charge ratio during the 3-day culture period, and prolonged exposure to hCG was without effect. Untreated follicles produced both oestrogen and androgens throughout the culture period. The addition of hCG resulted in a transitory stimulation in oestrogen secretion, inhibition of androgen secretion, and a marked and sustained rise in progestin secretion. It is proposed that the increase in glycolytic activity following exposure to hCG may relate to the activation of the granulosa cells coinciden... Mostrar Tudo |
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Palavras-Chave: |
Drug effects; Progestins. |
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Thesagro: |
Bioquímica; Endocrinologia; Fisiologia; Ovino; Reprodução. |
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Thesaurus Nal: |
Adenine nucleotides; Catechol estrogens; Chorionic gonadotropins; Glucose; Lactates; Ovarian follicles; Oxygen consumption; Pharmacology; Reproduction; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02703naa a2200373 a 4500
001 1522356
005 2023-03-29
008 1976 bl uuuu u00u1 u #d
024 7 $a10.1071/bi9760557.$2DOI
100 1 $aSEAMARK, R. F.
245 $aOxygen uptake, glucose utilization, lactate release and adenine nucleotide content of sheep pvarian follicles in culture$beffect of human chorionic gonadotrophin.$h[electronic resource]
260 $c1976
520 $aAbstract: A study has been made of the oxygen uptake, glucose utilization, lactate release and cellular content of adenine nucleotides of isolated sheep ovarian follicles (4-6 min in diameter) maintained in organ culture, and of the effects on these parameters of the addition of human chorionic gonadotrophin (hCG). The mean oxygen consumption of the entire follicles when freshly isolated and of the theca and membrana components was 0-56, 1-08 and 0-05 mumol per milligram wet weight of tissue per hour respectively. About 8 mumol of glucose was taken up and 16 mumol of lactate released per milligram wet weight of follicular tissue per hour during the first 24-h period of culture. This rate reduced by about 30% for each subsequent day of culture, but was significantly increased by the addition of hCG. The mean ATP content of theca and granulosa tissues was 4-6 and 2-8 nmol/mg wet weight of tissue respectively. There was no discernable change in tissue adenine nucleotide content or energy charge ratio during the 3-day culture period, and prolonged exposure to hCG was without effect. Untreated follicles produced both oestrogen and androgens throughout the culture period. The addition of hCG resulted in a transitory stimulation in oestrogen secretion, inhibition of androgen secretion, and a marked and sustained rise in progestin secretion. It is proposed that the increase in glycolytic activity following exposure to hCG may relate to the activation of the granulosa cells coincident with a transference of steroid synthetic capacity from theca interna to membrana granulosa.
650 $aAdenine nucleotides
650 $aCatechol estrogens
650 $aChorionic gonadotropins
650 $aGlucose
650 $aLactates
650 $aOvarian follicles
650 $aOxygen consumption
650 $aPharmacology
650 $aReproduction
650 $aSheep
650 $aBioquímica
650 $aEndocrinologia
650 $aFisiologia
650 $aOvino
650 $aReprodução
653 $aDrug effects
653 $aProgestins
700 1 $aAMATO, F.
700 1 $aHENDRICKSON, S.
700 1 $aMOOR, R. M.
773 $tAustralian Journal Biology Science$gv. 29, n. 5/6, p. 557-563, Dec. 1976.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
09/01/2015 |
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Data da última atualização: |
19/02/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
DIAS, N. dos S.; MIRANDA, C. M.; QUEIROZ, I. dos S.; BARBOSA, A. C. O.; PESTANA, K. N.; SILVA, P. H.; SANTOS, R. M. F.; OLIVEIRA, E. J. de; SANTOS, V. da S.; FERREIRA, C. F. |
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Afiliação: |
NAIRA DOS SANTOS DIAS, UFRB; CAROLINA MACEDO MIRANDA, UFRB; IANE DOS SANTOS QUEIROZ, UFRB; ANA CLAUDIA OLIVEIRA BARBOSA, UFRB; KÁTIA NOGUEIRA PESTANA; PAULO HENRIQUE SILVA, UFRB; ROGÉRIO MERCES FERREIRA SANTOS, UFRB; EDER JORGE DE OLIVEIRA, CNPMF; VANDERLEI DA SILVA SANTOS, CNPMF; CLAUDIA FORTES FERREIRA, CNPMF. |
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Título: |
Utilização de PCR multiplex a partir de marcadores VNTR em mandioca. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 8., 2014, Cruz das Almas, Ba. Pesquisa: despertando mentes para a inovação e transformando o futuro :[anais]. Cruz das Almas, Ba, Embrapa Mandioca e Fruticultura, 2014. |
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Idioma: |
Português |
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Conteúdo: |
O uso de marcadores moleculares do tipo VNTR (Variable Number of Tandem Repeats) no equipamento Fragment Analyzer ? Automated TE System da Advanced Analytical,Bocasiona custos elevados por unidade de dado a ser analisado. |
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Palavras-Chave: |
Genetic marker. |
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Thesagro: |
Mandioca; Marcador molecular. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139489/1/086-14.pdf
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Marc: |
LEADER 01129nam a2200253 a 4500
001 2005004
005 2016-02-19
008 2014 bl uuuu u00u1 u #d
100 1 $aDIAS, N. dos S.
245 $aUtilização de PCR multiplex a partir de marcadores VNTR em mandioca.$h[electronic resource]
260 $aIn: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 8., 2014, Cruz das Almas, Ba. Pesquisa: despertando mentes para a inovação e transformando o futuro :[anais]. Cruz das Almas, Ba, Embrapa Mandioca e Fruticultura$c2014
520 $aO uso de marcadores moleculares do tipo VNTR (Variable Number of Tandem Repeats) no equipamento Fragment Analyzer ? Automated TE System da Advanced Analytical,Bocasiona custos elevados por unidade de dado a ser analisado.
650 $aMandioca
650 $aMarcador molecular
653 $aGenetic marker
700 1 $aMIRANDA, C. M.
700 1 $aQUEIROZ, I. dos S.
700 1 $aBARBOSA, A. C. O.
700 1 $aPESTANA, K. N.
700 1 $aSILVA, P. H.
700 1 $aSANTOS, R. M. F.
700 1 $aOLIVEIRA, E. J. de
700 1 $aSANTOS, V. da S.
700 1 $aFERREIRA, C. F.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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