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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
02/05/2023 |
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Autoria: |
SZELL, A.; SHELTON, J. N.; SZELL, K. |
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Título: |
Osmotic characteristics of sheep and cattle embryos. |
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Ano de publicação: |
1989 |
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Fonte/Imprenta: |
Cryobiology, v. 26, n. 3, p.297-301, 1989. |
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DOI: |
10.1016/0011-2240(89)90026-6 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Ten minutes of exposure to increasing concentrations of sucrose caused a proportional decrease in the volume of sheep late morulae, their relative volume changed as a linear function of the reciprocal of the osmolality of the medium. Day 6 sheep and Day 7 cattle embryos responded to the addition of permeating cryoprotectants by an initial shrinkage which was followed by gradual reexpansion. After 1.25 min exposure the relative volumes of sheep and cattle embryos respectively were 20 and 25% smaller in glycerol than in ethylene glycol. The volumes of cattle and sheep embryos remained smaller in glycerol than in ethylene glycol up to the final observation at 30 min. The osmotic response of sheep late morulae to 2.0 M propylene glycol was intermediate between their response to 2.0 M glycerol and to 2.0 M ethylene glycol. These results indicate that Day 6 sheep and Day 7 cattle embryos are more permeable to ethylene glycol than to glycerol. |
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Palavras-Chave: |
Cryoprotective agents; Osmolar concentration. |
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Thesagro: |
Criopreservação; Fisiologia; Ovino; Reprodução. |
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Thesaurus Nal: |
Cattle; Freezing; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01662naa a2200265 a 4500
001 1522272
005 2023-05-02
008 1989 bl uuuu u00u1 u #d
024 7 $a10.1016/0011-2240(89)90026-6$2DOI
100 1 $aSZELL, A.
245 $aOsmotic characteristics of sheep and cattle embryos.$h[electronic resource]
260 $c1989
520 $aAbstract: Ten minutes of exposure to increasing concentrations of sucrose caused a proportional decrease in the volume of sheep late morulae, their relative volume changed as a linear function of the reciprocal of the osmolality of the medium. Day 6 sheep and Day 7 cattle embryos responded to the addition of permeating cryoprotectants by an initial shrinkage which was followed by gradual reexpansion. After 1.25 min exposure the relative volumes of sheep and cattle embryos respectively were 20 and 25% smaller in glycerol than in ethylene glycol. The volumes of cattle and sheep embryos remained smaller in glycerol than in ethylene glycol up to the final observation at 30 min. The osmotic response of sheep late morulae to 2.0 M propylene glycol was intermediate between their response to 2.0 M glycerol and to 2.0 M ethylene glycol. These results indicate that Day 6 sheep and Day 7 cattle embryos are more permeable to ethylene glycol than to glycerol.
650 $aCattle
650 $aFreezing
650 $aSheep
650 $aCriopreservação
650 $aFisiologia
650 $aOvino
650 $aReprodução
653 $aCryoprotective agents
653 $aOsmolar concentration
700 1 $aSHELTON, J. N.
700 1 $aSZELL, K.
773 $tCryobiology$gv. 26, n. 3, p.297-301, 1989.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
