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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
03/01/2024 |
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Autoria: |
DOTT, H. M.; WHITE, I. G. |
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Título: |
Effect of potassium on ram spermatozoa studied by a flow dialysis technique. |
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Ano de publicação: |
1964 |
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Fonte/Imprenta: |
Journal of Reproduction and Fertility, v. 7, p. 127-138, Apr., 1964. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The continuous flow dialysis apparatus (cfda) has been used to investigate the importance ofpotassium for the maintenance of the activity of ram spermatozoa. Ram spermatozoa contain 136 mg K+/100 g and the seminal plasma 78 mg K+/100 g. When semen is diluted one in three with a phosphate diluent, there is a rapid fall in the potassium concentration within the spermatozoa. Dialysis in the cfda causes a further exponential fall unless potassium is included in the dialysis fluid. Unless the phosphate buffer used in the cfda contains potassium ions, there is a sudden decrease in the impedance-change frequency (icf) between 3 and 5 hr from the start of dialysis. Washing or keeping unwashed spermatozoa for 2 hr prior to dialysis does not alter this response or the time ofdialysis at which it takes place. Semen diluted one in three with fresh seminal plasma or with plasma obtained from stored semen gave the same response (fall in icf). The decline in icf due to the absence of potassium was less rapid when undiluted semen was used. Semen washed with a bicarbonate buffer had a lower initial icf than semen washed with phosphate, but the icf declined more slowly, and in the absence of potassium there was no sudden drop in the icf. Samples with potassium maintained a higher icf than those without. The most marked potassium effect was obtained under aerobic conditions using a phosphate fluid containing fructose, lactate or acetate. Under anaerobic conditions the presence ofpotassium ions in the dialysis fluid had little or no effect on icf. MenosAbstract: The continuous flow dialysis apparatus (cfda) has been used to investigate the importance ofpotassium for the maintenance of the activity of ram spermatozoa. Ram spermatozoa contain 136 mg K+/100 g and the seminal plasma 78 mg K+/100 g. When semen is diluted one in three with a phosphate diluent, there is a rapid fall in the potassium concentration within the spermatozoa. Dialysis in the cfda causes a further exponential fall unless potassium is included in the dialysis fluid. Unless the phosphate buffer used in the cfda contains potassium ions, there is a sudden decrease in the impedance-change frequency (icf) between 3 and 5 hr from the start of dialysis. Washing or keeping unwashed spermatozoa for 2 hr prior to dialysis does not alter this response or the time ofdialysis at which it takes place. Semen diluted one in three with fresh seminal plasma or with plasma obtained from stored semen gave the same response (fall in icf). The decline in icf due to the absence of potassium was less rapid when undiluted semen was used. Semen washed with a bicarbonate buffer had a lower initial icf than semen washed with phosphate, but the icf declined more slowly, and in the absence of potassium there was no sudden drop in the icf. Samples with potassium maintained a higher icf than those without. The most marked potassium effect was obtained under aerobic conditions using a phosphate fluid containing fructose, lactate or acetate. Under anaerobic conditions the presence ofpota... Mostrar Tudo |
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Palavras-Chave: |
Bioquimica seminal; Tecnologia do semen. |
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Thesagro: |
Espermatozóide; Ovino; Potássio; Reprodução. |
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Thesaurus Nal: |
Acetates; Bicarbonates; Cations; Dialysis; Fructose; Metabolism; Pharmacology; Phosphates; Potassium; Rams; Reproduction; Sheep; Spermatozoa. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02498naa a2200361 a 4500
001 1522245
005 2024-01-03
008 1964 bl uuuu u00u1 u #d
100 1 $aDOTT, H. M.
245 $aEffect of potassium on ram spermatozoa studied by a flow dialysis technique.$h[electronic resource]
260 $c1964
520 $aAbstract: The continuous flow dialysis apparatus (cfda) has been used to investigate the importance ofpotassium for the maintenance of the activity of ram spermatozoa. Ram spermatozoa contain 136 mg K+/100 g and the seminal plasma 78 mg K+/100 g. When semen is diluted one in three with a phosphate diluent, there is a rapid fall in the potassium concentration within the spermatozoa. Dialysis in the cfda causes a further exponential fall unless potassium is included in the dialysis fluid. Unless the phosphate buffer used in the cfda contains potassium ions, there is a sudden decrease in the impedance-change frequency (icf) between 3 and 5 hr from the start of dialysis. Washing or keeping unwashed spermatozoa for 2 hr prior to dialysis does not alter this response or the time ofdialysis at which it takes place. Semen diluted one in three with fresh seminal plasma or with plasma obtained from stored semen gave the same response (fall in icf). The decline in icf due to the absence of potassium was less rapid when undiluted semen was used. Semen washed with a bicarbonate buffer had a lower initial icf than semen washed with phosphate, but the icf declined more slowly, and in the absence of potassium there was no sudden drop in the icf. Samples with potassium maintained a higher icf than those without. The most marked potassium effect was obtained under aerobic conditions using a phosphate fluid containing fructose, lactate or acetate. Under anaerobic conditions the presence ofpotassium ions in the dialysis fluid had little or no effect on icf.
650 $aAcetates
650 $aBicarbonates
650 $aCations
650 $aDialysis
650 $aFructose
650 $aMetabolism
650 $aPharmacology
650 $aPhosphates
650 $aPotassium
650 $aRams
650 $aReproduction
650 $aSheep
650 $aSpermatozoa
650 $aEspermatozóide
650 $aOvino
650 $aPotássio
650 $aReprodução
653 $aBioquimica seminal
653 $aTecnologia do semen
700 1 $aWHITE, I. G.
773 $tJournal of Reproduction and Fertility$gv. 7, p. 127-138, Apr., 1964.
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