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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
27/04/2023 |
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Autoria: |
DOTT, H. M. |
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Título: |
Results of inseminations with ram spermatozoa in different media. |
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Ano de publicação: |
1964 |
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Fonte/Imprenta: |
Journal Reproduction and Fertility, v. 8, n. 2, p. 257-258, 1964. |
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DOI: |
10.1530/jrf.0.0080257 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Previous studies have shown that stored ram semen (ram spermatozoa kept in contact with seminal plasma for 4\m=1/2\hr at 30\s=deg\C) is less likely to cause conception when inseminated into a ewe than fresh semen with a lower impedance change frequency (Dott, 1961). In the same paper it was shown that epididymal semen could be stored at 30\s=deg\C and ejaculated semen at 20\s=deg\C for 4\m=1/2\hr without causing a serious drop in the conception rate obtained with them. It could be concluded from these experiments that some factor in the seminal plasma adversely affected the fertilizing ability of ram spermatozoa. This effect is dependent on time and temperature but it is not known whether it is dependent on the continued presence of seminal plasma. Ewes have been inseminated with ejaculated ram spermatozoa separated from the seminal plasma, as the first in a series ofexperiments to test this. In these preliminary experiments the spermatozoa were freed of seminal plasma by a washing procedure, the first stage ofwhich was a 1 : 1 dilution and the final theoretical dilution of the constituents of the seminal plasma was 1 : 2592. This was brought about in four identical cycles of dilution and centrifugation at about 300 g. In practice the theoretical dilution is not achieved for constituents of the seminal plasma (Dott & Walton, 1960). The sperm density of the final suspension was the same as in the ejaculated semen. The effects offour washing fluids were studied, two phosphate buffers and two bicarbonate buffers. The composition of the phosphate buffers was (i) sodium phosphate: 0\m=.\128m-NaCl, 0\m=.\0204m-Na2HPO4.12H2O, 0\m=.\0048m-NaH2PO4. 2H2O; (ii) sodium potassium phosphate: 0\m=.\124m-Nacl, 0\m=.\005m-KCl, 0\m=.\0204m-Na2HPO4.12H2O, 0\m=.\0048m-NaH2PO4.2H2O and the bicarbonate buffers contained (iii) sodium bicarbonate: 0\m=.\128m-Nacl, 0\m=.\026m-NaHCO3; (iv) sodium potassium bicarbonate; 0\m=.\124m-NaCl, 0\m=.\005m-KCl, 0\m=.\026 m-NaHCO3. All were adjusted to pH 7\m=.\3. After washing in sodium potassium bicarbonate, the spermatozoa were packed into a pellet which was more difficult to disperse than the loose aggregations obtained in the other three solutions. The results of insemination are given in Table 1 and an overall X2 analysis showed that the spermatozoa washed in phosphate buffer produced a higher MenosAbstract: Previous studies have shown that stored ram semen (ram spermatozoa kept in contact with seminal plasma for 4\m=1/2\hr at 30\s=deg\C) is less likely to cause conception when inseminated into a ewe than fresh semen with a lower impedance change frequency (Dott, 1961). In the same paper it was shown that epididymal semen could be stored at 30\s=deg\C and ejaculated semen at 20\s=deg\C for 4\m=1/2\hr without causing a serious drop in the conception rate obtained with them. It could be concluded from these experiments that some factor in the seminal plasma adversely affected the fertilizing ability of ram spermatozoa. This effect is dependent on time and temperature but it is not known whether it is dependent on the continued presence of seminal plasma. Ewes have been inseminated with ejaculated ram spermatozoa separated from the seminal plasma, as the first in a series ofexperiments to test this. In these preliminary experiments the spermatozoa were freed of seminal plasma by a washing procedure, the first stage ofwhich was a 1 : 1 dilution and the final theoretical dilution of the constituents of the seminal plasma was 1 : 2592. This was brought about in four identical cycles of dilution and centrifugation at about 300 g. In practice the theoretical dilution is not achieved for constituents of the seminal plasma (Dott & Walton, 1960). The sperm density of the final suspension was the same as in the ejaculated semen. The effects offour washing fluids were studied, two ... Mostrar Tudo |
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Thesagro: |
Inseminação Artificial; Ovino; Reprodução. |
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Thesaurus Nal: |
Artificial insemination; Reproduction; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02965naa a2200205 a 4500
001 1521995
005 2023-04-27
008 1964 bl uuuu u00u1 u #d
024 7 $a10.1530/jrf.0.0080257$2DOI
100 1 $aDOTT, H. M.
245 $aResults of inseminations with ram spermatozoa in different media.$h[electronic resource]
260 $c1964
520 $aAbstract: Previous studies have shown that stored ram semen (ram spermatozoa kept in contact with seminal plasma for 4\m=1/2\hr at 30\s=deg\C) is less likely to cause conception when inseminated into a ewe than fresh semen with a lower impedance change frequency (Dott, 1961). In the same paper it was shown that epididymal semen could be stored at 30\s=deg\C and ejaculated semen at 20\s=deg\C for 4\m=1/2\hr without causing a serious drop in the conception rate obtained with them. It could be concluded from these experiments that some factor in the seminal plasma adversely affected the fertilizing ability of ram spermatozoa. This effect is dependent on time and temperature but it is not known whether it is dependent on the continued presence of seminal plasma. Ewes have been inseminated with ejaculated ram spermatozoa separated from the seminal plasma, as the first in a series ofexperiments to test this. In these preliminary experiments the spermatozoa were freed of seminal plasma by a washing procedure, the first stage ofwhich was a 1 : 1 dilution and the final theoretical dilution of the constituents of the seminal plasma was 1 : 2592. This was brought about in four identical cycles of dilution and centrifugation at about 300 g. In practice the theoretical dilution is not achieved for constituents of the seminal plasma (Dott & Walton, 1960). The sperm density of the final suspension was the same as in the ejaculated semen. The effects offour washing fluids were studied, two phosphate buffers and two bicarbonate buffers. The composition of the phosphate buffers was (i) sodium phosphate: 0\m=.\128m-NaCl, 0\m=.\0204m-Na2HPO4.12H2O, 0\m=.\0048m-NaH2PO4. 2H2O; (ii) sodium potassium phosphate: 0\m=.\124m-Nacl, 0\m=.\005m-KCl, 0\m=.\0204m-Na2HPO4.12H2O, 0\m=.\0048m-NaH2PO4.2H2O and the bicarbonate buffers contained (iii) sodium bicarbonate: 0\m=.\128m-Nacl, 0\m=.\026m-NaHCO3; (iv) sodium potassium bicarbonate; 0\m=.\124m-NaCl, 0\m=.\005m-KCl, 0\m=.\026 m-NaHCO3. All were adjusted to pH 7\m=.\3. After washing in sodium potassium bicarbonate, the spermatozoa were packed into a pellet which was more difficult to disperse than the loose aggregations obtained in the other three solutions. The results of insemination are given in Table 1 and an overall X2 analysis showed that the spermatozoa washed in phosphate buffer produced a higher
650 $aArtificial insemination
650 $aReproduction
650 $aSheep
650 $aInseminação Artificial
650 $aOvino
650 $aReprodução
773 $tJournal Reproduction and Fertility$gv. 8, n. 2, p. 257-258, 1964.
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Embrapa Caprinos e Ovinos (CNPC) |
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| 3. |  | BEVITORI, R.; TOGAWA, R. C.; COSTA, M. M.; GRYNBERG, P.; GUIMARÃES, L. S. M.; ABREU, A. G. de; MELLO, R.; GROSSI DE SÁ, M. F. Transcriptome profiling of two near-isogenic rice lines infected by Magnaporthe oryzae using RNA-SEQ. In: CONFERENCIA INTERNACIONAL DO ARROZ PARA AMÉRICA LATINA E CARIBE, 12., 2015, Porto Alegre. Horizonte para a competitividade: anais. Porto Alegre: IRGA, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 4. | | BEVITORI, R.; TOGAWA, R. C.; COSTA, M. M.; GRYNBERG, P.; GUIMARÃES, L. S. M.; ABREU, A. G. de; MELLO, R.; GROSSI DE SÁ, M. F. Transcriptome profiling of twonear-isogenic rice lines infected by Magnaporthe oryzae using RNA-SEQ. In: CONFERENCIA INTERNACIONAL DO ARROZ PARA AMÉRICA LATINA E CARIBE, 12., 2015, Porto Alegre. Horizonte para a competitividade: anais. Porto Alegre: IRGA, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 4 | |
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