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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
09/05/2023 |
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Autoria: |
EMIK, L. O.; SIDWELL, G. M. |
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Título: |
Factors affecting the estimation of concentration of sperm in ram's semen by the photoelectrometric method. |
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Ano de publicação: |
1947 |
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Fonte/Imprenta: |
Journal of Animal Science, v. 6, n. 4, p. 467-475, Nov. 1947. |
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DOI: |
https://doi.org/10.2527/jas1947.64467x |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Over 300 ejaculates from about 100 rams were exmained at the U. S. Sheep Experiment Station and Western Sheep Breeding Laboratory during annual Fall testing of rams. The relations between counts of sperm made with a Spencer Brightline hemacytometer and turbidity readings made with a Klett-Sommerson photoelectric colorimeter were analyzed by regression and covariance methods. Diluting fluids had significantly different effects on opacity of the diluted semen. Chlorazene was the diluter of choice, despite its change upon prolonged standing, because it was the only diluter capable of dissolving clumps which were prevalent in rams' semen. A dilution of 1:200 gave the optimum range of readings in the colorimeter scale, lower effect of debris, and lower error of estimate. Scores were established for estimating the amount of visible debris in the samples. Highly significant improvement in colorimetric estimation of sperm concentration was effected by scoring the amount of debris. Non-visible elements of debris, measured as residual turbidity after centrifugation of diluted samples, contributed appreciably to the colorimeter readings. This residual turbidity could not be used as an estimate of total debris. The colorimeter readings of semen with the debris removed gave a regression line with the intercept point not significantly different from zero. Two brands of diluting pipettes showed no differences in variability when rinsed into the diluted sample. They did show significant differences in volume and ability to drain without rinsing. The technique adopted for use (chlorazene at: 1200 with scoring of debris) gave a standard error of estimate of 38.1 (107 sperm per ml.). The error due to workers and instruments was negligible. The standard deviations for the two instruments were similar, 21.4 and 19.7, for sampling and dilution, but the hemacytometer was slightly less accurate, 26.7, because of its higher variance due to the Poisson distributional error of the smaller volume measured. The error of estimate attributable to scoring of samples for amount of debris was 22.7, while the error for debris and heterogeneity which could not be scored was 34.1. MenosAbstract: Over 300 ejaculates from about 100 rams were exmained at the U. S. Sheep Experiment Station and Western Sheep Breeding Laboratory during annual Fall testing of rams. The relations between counts of sperm made with a Spencer Brightline hemacytometer and turbidity readings made with a Klett-Sommerson photoelectric colorimeter were analyzed by regression and covariance methods. Diluting fluids had significantly different effects on opacity of the diluted semen. Chlorazene was the diluter of choice, despite its change upon prolonged standing, because it was the only diluter capable of dissolving clumps which were prevalent in rams' semen. A dilution of 1:200 gave the optimum range of readings in the colorimeter scale, lower effect of debris, and lower error of estimate. Scores were established for estimating the amount of visible debris in the samples. Highly significant improvement in colorimetric estimation of sperm concentration was effected by scoring the amount of debris. Non-visible elements of debris, measured as residual turbidity after centrifugation of diluted samples, contributed appreciably to the colorimeter readings. This residual turbidity could not be used as an estimate of total debris. The colorimeter readings of semen with the debris removed gave a regression line with the intercept point not significantly different from zero. Two brands of diluting pipettes showed no differences in variability when rinsed into the diluted sample. They did show signi... Mostrar Tudo |
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Palavras-Chave: |
Biochemical Phenomena; Tecnologia do semen. |
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Thesagro: |
Espermatozóide; Ovino; Reprodução; Sêmen. |
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Thesaurus Nal: |
Body fluids; Glycogen; Phosphoric monoester hydrolases; Sheep; Spermatozoa. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03027naa a2200277 a 4500
001 1521526
005 2023-05-09
008 1947 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.2527/jas1947.64467x$2DOI
100 1 $aEMIK, L. O.
245 $aFactors affecting the estimation of concentration of sperm in ram's semen by the photoelectrometric method.$h[electronic resource]
260 $c1947
520 $aAbstract: Over 300 ejaculates from about 100 rams were exmained at the U. S. Sheep Experiment Station and Western Sheep Breeding Laboratory during annual Fall testing of rams. The relations between counts of sperm made with a Spencer Brightline hemacytometer and turbidity readings made with a Klett-Sommerson photoelectric colorimeter were analyzed by regression and covariance methods. Diluting fluids had significantly different effects on opacity of the diluted semen. Chlorazene was the diluter of choice, despite its change upon prolonged standing, because it was the only diluter capable of dissolving clumps which were prevalent in rams' semen. A dilution of 1:200 gave the optimum range of readings in the colorimeter scale, lower effect of debris, and lower error of estimate. Scores were established for estimating the amount of visible debris in the samples. Highly significant improvement in colorimetric estimation of sperm concentration was effected by scoring the amount of debris. Non-visible elements of debris, measured as residual turbidity after centrifugation of diluted samples, contributed appreciably to the colorimeter readings. This residual turbidity could not be used as an estimate of total debris. The colorimeter readings of semen with the debris removed gave a regression line with the intercept point not significantly different from zero. Two brands of diluting pipettes showed no differences in variability when rinsed into the diluted sample. They did show significant differences in volume and ability to drain without rinsing. The technique adopted for use (chlorazene at: 1200 with scoring of debris) gave a standard error of estimate of 38.1 (107 sperm per ml.). The error due to workers and instruments was negligible. The standard deviations for the two instruments were similar, 21.4 and 19.7, for sampling and dilution, but the hemacytometer was slightly less accurate, 26.7, because of its higher variance due to the Poisson distributional error of the smaller volume measured. The error of estimate attributable to scoring of samples for amount of debris was 22.7, while the error for debris and heterogeneity which could not be scored was 34.1.
650 $aBody fluids
650 $aGlycogen
650 $aPhosphoric monoester hydrolases
650 $aSheep
650 $aSpermatozoa
650 $aEspermatozóide
650 $aOvino
650 $aReprodução
650 $aSêmen
653 $aBiochemical Phenomena
653 $aTecnologia do semen
700 1 $aSIDWELL, G. M.
773 $tJournal of Animal Science$gv. 6, n. 4, p. 467-475, Nov. 1947.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 12 | |
| 1. |  | ROSA, P. M. S.; MONTEIRO, C. A. S.; LEAL, G. R.; CAMARGO, A. J. R.; SERAPIÃO, R. V.; LIMA, M. R.; GARCIA, J. M.; OLIVEIRA, C. S. Association of two epigenetic modulators on the initial development of female bovine embryos. Animal Reproduction, v. 15, n. 3, p. 484, 2018. Edição dos proceedings do 32º Annual Meeting of the Brazilian Embryo Technology Society, Florianópolis, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 2. | | LEAL, G. R.; GRACIOSA, M. A. G.; PASOLINI, R.; MONTEIRO, C. A. S.; CAMARGO, A. J. Dos R.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Adaptação do sistema SPOM melhora a taxa de produção in vitro de embriões bovinos. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 3. | | BARROS, B. A. F. de; ROSA, P. M. da S.; MONTEIRO, C. A. S.; LEAL, G. R.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Desenvolvimento e validação de novo sistema de cultivo individual de embriões bovinos produzidos in vitro. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 18., 2016, Juiz de Fora, MG. Anais... Juiz de Fora: Embrapa Gado de Leite, 2016. (Embrapa Gado de Leite. Documentos, 197).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 4. | | OLIVEIRA, C. S.; BARROS, B. A. F. de; MONTEIRO, C. A. S.; ROSA, P. M. S.; LEAL, G. R.; SERAPIÃO, R. V.; CAMARGO, L. S. de A. Individual assessment of bovine embryo development using a homemade chamber reveals kinetic patterns of success and failure to reach blastocyst stage. Systems Biology in Reproductive Medicine, v. 65, n. 4, p. 301-311, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 5. | | MONTEIRO, C. A. S.; BARROS, B. A. F. De; ROSA, P. A. Da S.; LEAL, G. R.; CAMARGO, A. J. Dos R.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Taxa de apoptose após vitrificação/ reaquecimento de embriões Gir produzidos in vitro. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016. 1| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 6. | | LEAL, G. R.; OLIVEIRA, C. S.; MONTEIRO, C. A. S.; SARAIVA, H. F. R. A.; ROSA, P. M. S.; CAMARGO, A. J. R.; RODRIGUES, A. L. R.; VASCONCELOS, C. O. P.; NOGUEIRA, L. A. G.; SERAPIÃO, R. V. Effect of in vitro SPOM maturation system use on bovine embryos lipid score. Animal Reproduction, v. 12, n. 3, 2015. Edição dos proceedings do 29º Annual Meeting of the Brazilian Embryo Technology Society, 2015, Gramado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | MONTEIRO, C. A. S.; LEAL, G. R.; SARAIVA, H. F. R. A.; CAMARGO, A. J. R.; RODRIGUES, A. L. R.; SERAPIÃO, R. V.; NOGUEIRA, L. A. G.; CAMARGO, L. S. de A.; GARCIA, J. M.; OLIVEIRA, C. S. Effect of cAMP modulators during oocyte in vitro maturation on nuclear maturation and cytoskeleton integrity of vitrified bovine oocytes. In: ANNUAM MEETING OF THE BRAZILIAN EMBRYO TECHNOLOGY SOCIETY, 29., 2015, Gramado. Abstracts... Gramado: SBTE, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 8. | | SARAIVA, H. F. R. A.; LEAL, G. R.; MONTEIRO, C. A. S.; OLIVEIRA, C. S.; ROSA, P. M. S.; CAMARGO, A. J. R.; SERAPIÃO, R. V.; BRANDÃO, F. Z. Effect of camp modulators on in vitro pre maturation in production rate and lipid content of crossbred bovine embryos. Animal Reproduction, v. 12, n. 3, 2015. Edição dos proceedings do 29º Annual Meeting of the Brazilian Embryo Technology Society, 2015, Gramado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 9. | | LEAL, G. R.; MONTEIRO, C. A. S.; SARAIVA, H. P. F. R. A.; CAMARGO, A. J. R.; ROSA, P. M. S.; RODRIGUES, A. L. R.; VASCONCELOS, C. O. P.; NOGUEIRA, L. A. G.; OLIVEIRA, C. S.; SERAPIÃO, R. V. Effect of cyclic adenosine monophosphate modulators during oocyte in vitro maturation on bovine embryos (Gyr x Holstein) quality. In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 2016, Louisville. Reproduction, fertility and development: proceedings. Louisville: [s.n.], 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 10. | | SARAIVA, H. F. R. A.; MONTEIRO, C. A. S.; LEAL, G. R.; CAMARGO, A. J. R.; RODRIGUES, A. L. R.; VASCONCELOS, C. O. P.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Influence of breed composition of crossbred cows between Gir and Holstein breeds in performance donors. Animal Reproduction, v. 11, n. 3, p. 423, 2014. Edição dos proceedings do 28º Annual Meeting of the Brazilian Embryo Technology Society, 2014, Natal.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 11. | | OLIVEIRA, C. S.; ROSA, P. M. da S.; ALVES, B. R. C.; MONTEIRO, C. A. S.; LEAL, G. R.; GUEDES, P. H. E.; CAMARGO, A. J. dos R.; SARAIVA, N. Z. Luteinizing hormone secretion, ovulatory capacity, and oocyte quality in peripubertal Gir heifers. Zygote, v. 31, n. 4, p. 342-349, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 12. | | LEAL, G. R.; GRACIOSA, M. A. G.; MONTEIRO, C. A. S.; PASOLINI, R.; CAMARGO, A. J. dos R.; OLIVEIRA, C. S.; VASCONCELOS, C. O. de P.; NOGUEIRA, L. A. G.; FERREIRA, A. M. R.; SERAPIAO, R. V. The SPOM-adapted IVM system improves in vitro production of bovine embryos. Theriogenology, v. 158, p. 277-282, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 12 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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