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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
02/05/2023 |
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Autoria: |
CHIN, J. C.; PANO, B.; CARRIGAN, M. |
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Título: |
Comparison of seroreactivity of rams with brucellosis in a complement fixation test, whole cell ELISA and by immunoblotting. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Veterinary Microbiology, v. 26, n. 3, p. 291-299, 1991. |
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DOI: |
10.1016/0378-1135(91)90022-8 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: In rams with ovine brucellosis, a high degree of serological correlation exists between the complement fixation (CF) test which utilises antigen extracted from bacteria with hot saline, and the ELISA reactivity using methanol-fixed Brucella ovis as the assay reagent. Since the whole cell ELISA (CELISA) detects mainly antibodies against surface antigens of B. ovis, it was concluded that the similar findings of the two serological tests is due in part to the presence of membrane antigens in the CF test antigen following hot saline extraction of intact bacteria. Immunoblots with pooled sera representing different CF titres confirmed that the major immunoreactive antigens of B. ovis were located in four zones: alpha, beta, gamma 1 and 2 with corresponding apparent molecular masses of 55 and 60 kDa; 27 and 29 kDa; 18.5-20 kDa and 17-18 kDa, respectively. These zones of reactivity were consistently present in immunoblots when assayed against different B. ovis isolates even though Coomassie brilliant blue staining of SDS-PAGE gels revealed some differences in polypeptide banding patterns. However, these intensely-stained CBB bands located at 38 and 40 kDa which distinguished three of the seven B. ovis isolates were considerably less reactive in immunoblots compared to polypeptides that were located at positions equivalent to alpha, beta or gamma reactivities. Intensity of immunoblot reactivity against polypeptides located in the alpha, beta and gamma zones intensified with increasing CF titre. Sera with CF titres greater than 32 also tended to react against bands of higher apparent molecular masses located at 65, 70, 73, 78, 80 and 86 kD MenosAbstract: In rams with ovine brucellosis, a high degree of serological correlation exists between the complement fixation (CF) test which utilises antigen extracted from bacteria with hot saline, and the ELISA reactivity using methanol-fixed Brucella ovis as the assay reagent. Since the whole cell ELISA (CELISA) detects mainly antibodies against surface antigens of B. ovis, it was concluded that the similar findings of the two serological tests is due in part to the presence of membrane antigens in the CF test antigen following hot saline extraction of intact bacteria. Immunoblots with pooled sera representing different CF titres confirmed that the major immunoreactive antigens of B. ovis were located in four zones: alpha, beta, gamma 1 and 2 with corresponding apparent molecular masses of 55 and 60 kDa; 27 and 29 kDa; 18.5-20 kDa and 17-18 kDa, respectively. These zones of reactivity were consistently present in immunoblots when assayed against different B. ovis isolates even though Coomassie brilliant blue staining of SDS-PAGE gels revealed some differences in polypeptide banding patterns. However, these intensely-stained CBB bands located at 38 and 40 kDa which distinguished three of the seven B. ovis isolates were considerably less reactive in immunoblots compared to polypeptides that were located at positions equivalent to alpha, beta or gamma reactivities. Intensity of immunoblot reactivity against polypeptides located in the alpha, beta and gamma zones intensified wit... Mostrar Tudo |
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Palavras-Chave: |
Brucella analysis. |
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Thesagro: |
Brucelose; Diagnostico; Doença; Epidemia; Ovino. |
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Thesaurus Nal: |
Brucellosis; Dielectrophoresis; Enzyme-linked immunosorbent assay; Immunoblotting; Immunology; Peptides; Polyacrylamide gel electrophoresis; Sheep diseases; Surface antigens. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02665naa a2200337 a 4500
001 1520397
005 2023-05-02
008 1991 bl uuuu u00u1 u #d
024 7 $a10.1016/0378-1135(91)90022-8$2DOI
100 1 $aCHIN, J. C.
245 $aComparison of seroreactivity of rams with brucellosis in a complement fixation test, whole cell ELISA and by immunoblotting.$h[electronic resource]
260 $c1991
520 $aAbstract: In rams with ovine brucellosis, a high degree of serological correlation exists between the complement fixation (CF) test which utilises antigen extracted from bacteria with hot saline, and the ELISA reactivity using methanol-fixed Brucella ovis as the assay reagent. Since the whole cell ELISA (CELISA) detects mainly antibodies against surface antigens of B. ovis, it was concluded that the similar findings of the two serological tests is due in part to the presence of membrane antigens in the CF test antigen following hot saline extraction of intact bacteria. Immunoblots with pooled sera representing different CF titres confirmed that the major immunoreactive antigens of B. ovis were located in four zones: alpha, beta, gamma 1 and 2 with corresponding apparent molecular masses of 55 and 60 kDa; 27 and 29 kDa; 18.5-20 kDa and 17-18 kDa, respectively. These zones of reactivity were consistently present in immunoblots when assayed against different B. ovis isolates even though Coomassie brilliant blue staining of SDS-PAGE gels revealed some differences in polypeptide banding patterns. However, these intensely-stained CBB bands located at 38 and 40 kDa which distinguished three of the seven B. ovis isolates were considerably less reactive in immunoblots compared to polypeptides that were located at positions equivalent to alpha, beta or gamma reactivities. Intensity of immunoblot reactivity against polypeptides located in the alpha, beta and gamma zones intensified with increasing CF titre. Sera with CF titres greater than 32 also tended to react against bands of higher apparent molecular masses located at 65, 70, 73, 78, 80 and 86 kD
650 $aBrucellosis
650 $aDielectrophoresis
650 $aEnzyme-linked immunosorbent assay
650 $aImmunoblotting
650 $aImmunology
650 $aPeptides
650 $aPolyacrylamide gel electrophoresis
650 $aSheep diseases
650 $aSurface antigens
650 $aBrucelose
650 $aDiagnostico
650 $aDoença
650 $aEpidemia
650 $aOvino
653 $aBrucella analysis
700 1 $aPANO, B.
700 1 $aCARRIGAN, M.
773 $tVeterinary Microbiology$gv. 26, n. 3, p. 291-299, 1991.
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| 20. |  | DEL DUCA, L. de J. A.; GUARIENTI, E. M.; FONTANELI, R. S.; ZANOTTO, D. L. Influência de cortes simulando pastejo na composição química de grãos de cereais de inverno. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 34, n. 9, p. 1607-1614, set. 1999.| Biblioteca(s): Embrapa Trigo; Embrapa Unidades Centrais. |
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