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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
11/12/2023 |
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Autoria: |
PAGELS, W. R.; SACHS, R. J.; MARNETT, L. J.; DEWITT, D. L.; DAY, J. S.; SMITH, W. L. |
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Título: |
Immunochemical evidence for the involvement of prostaglandin H synthase in hydroperoxide-dependent oxidations by ram seminal vesicle microsomes. |
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Ano de publicação: |
1983 |
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Fonte/Imprenta: |
The Journal of Biological Chemistry, v. 258, n. 10, p. 6517-6523, 1983. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Monoclonal antibodies against prostaglandin H (PGH) synthase have been used to precipitate cyclooxygenase and peroxidase activities from detergent-solubilized preparations of ram seminal vesicle microsomes. Approximately 85% of the solubilized cyclooxygenase activity was precipitated using an excess of anti-PGH synthase antibody; under similar conditions, immunoprecipitation of 60% of the diphenylisobenzofuran peroxidase, 75% of the phenylbutazone peroxidase, and 50% of the epinephrine peroxidase activities occurred. In contrast, less than 10% of the cyclooxygenase or peroxidase activities could be precipitated with nonimmune, control antibody preparations. These data indicate that the hydroperoxidase activity of PGH synthase is the major peroxidase activity catalyzing the co-oxidation of xenobiotics in ram seminal vesicle microsomes. |
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Palavras-Chave: |
Prostaglandin-Endoperoxide Synthases. |
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Thesagro: |
Endocrinologia; Ovino; Reprodução; Staphylococcus Aureus. |
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Thesaurus Nal: |
Antibodies; Benzofurans; Endocrinology; Enzymology; Epinephrine; Hydrogen peroxide; Males; Microsomes; Phenylbutazone; Reproduction; Seminal vesicles; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01975naa a2200385 a 4500
001 1520203
005 2023-12-11
008 1983 bl uuuu u00u1 u #d
100 1 $aPAGELS, W. R.
245 $aImmunochemical evidence for the involvement of prostaglandin H synthase in hydroperoxide-dependent oxidations by ram seminal vesicle microsomes.$h[electronic resource]
260 $c1983
520 $aAbstract: Monoclonal antibodies against prostaglandin H (PGH) synthase have been used to precipitate cyclooxygenase and peroxidase activities from detergent-solubilized preparations of ram seminal vesicle microsomes. Approximately 85% of the solubilized cyclooxygenase activity was precipitated using an excess of anti-PGH synthase antibody; under similar conditions, immunoprecipitation of 60% of the diphenylisobenzofuran peroxidase, 75% of the phenylbutazone peroxidase, and 50% of the epinephrine peroxidase activities occurred. In contrast, less than 10% of the cyclooxygenase or peroxidase activities could be precipitated with nonimmune, control antibody preparations. These data indicate that the hydroperoxidase activity of PGH synthase is the major peroxidase activity catalyzing the co-oxidation of xenobiotics in ram seminal vesicle microsomes.
650 $aAntibodies
650 $aBenzofurans
650 $aEndocrinology
650 $aEnzymology
650 $aEpinephrine
650 $aHydrogen peroxide
650 $aMales
650 $aMicrosomes
650 $aPhenylbutazone
650 $aReproduction
650 $aSeminal vesicles
650 $aSheep
650 $aEndocrinologia
650 $aOvino
650 $aReprodução
650 $aStaphylococcus Aureus
653 $aProstaglandin-Endoperoxide Synthases
700 1 $aSACHS, R. J.
700 1 $aMARNETT, L. J.
700 1 $aDEWITT, D. L.
700 1 $aDAY, J. S.
700 1 $aSMITH, W. L.
773 $tThe Journal of Biological Chemistry$gv. 258, n. 10, p. 6517-6523, 1983.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Trigo; Embrapa Unidades Centrais. |
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Data corrente: |
13/11/2008 |
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Data da última atualização: |
17/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
HOMRICH, M. S.; PASSAGLIA, L. M. P.; PEREIRA, J. F.; BERTAGNOLLI, P. F.; SALVADORI, J. R.; NICOLAU, M.; KALTCHUK-SANTOS, E.; ALVES, L. B.; BODANESE-ZANETTINI, M. H. |
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Afiliação: |
Jorge Fernando Pereira, CNPT; PAULO FERNANDO BERTAGNOLLI, CNPT; José Roberto Salvadori, CNPT; MARCIO NICOLAU, CNPT. |
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Título: |
Agronomic performance, chromosomal stability and resistance to velvetbean caterpillar of transgenic soybean expressing cry1Ac gene. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 7, p. 801-807, jul. 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this work was to analyze the agronomic performance and chromosomal stability of transgenic homozygous progenies of soybean [Glycine max (L.) Merrill.], and to confirm the resistance of these plants against Anticarsia gemmatalis. Eleven progenies expressing cry1Ac, hpt and gusA genes were evaluated for agronomic characteristics in relation to the nontransformed parent IAS 5 cultivar. Cytogenetical analysis was carried out on transgenic and nontransgenic plants. Two out of the 11 transgenic progenies were also evaluated, in vitro and in vivo, for resistance to A. gemmatalis. Two negative controls were used in resistance bioassays: a transgenic homozygous line, containing only the gusA reporter gene, and nontransgenic 'IAS 5' plants. The presence of cry1Ac transgene affected neither the development nor the yield of plants. Cytogenetical analysis showed that transgenic plants presented normal karyotype. In detached-leaf bioassay, cry1Ac plants exhibited complete efficacy against A. gemmatalis, whereas negative controls were significantly damaged. Whole-plant feeding assay confirmed a very high protection of cry1Ac against velvetbean caterpillar, while nontransgenic 'IAS 5' plants and homozygous gusA line exhibited 56.5 and 71.5% defoliation, respectively. The presence of cry1Ac transgene doesn't affect the majority of agronomic traits (including yield) of soybean and grants high protection against A. gemmatalis. |
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Palavras-Chave: |
plant resistance to insects; resistência de plantas a insetos; transferência de genes. |
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Thesagro: |
Anticarsia Gemmatalis; Glycine Max; Soja. |
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Thesaurus NAL: |
gene transfer. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CNPT-2010/11249/1/43n07a03.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE-2009-09/44846/1/43n07a03.pdf
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Marc: |
LEADER 02410naa a2200301 a 4500
001 1823033
005 2021-08-17
008 2008 bl uuuu u00u1 u #d
100 1 $aHOMRICH, M. S.
245 $aAgronomic performance, chromosomal stability and resistance to velvetbean caterpillar of transgenic soybean expressing cry1Ac gene.
260 $c2008
520 $aThe objective of this work was to analyze the agronomic performance and chromosomal stability of transgenic homozygous progenies of soybean [Glycine max (L.) Merrill.], and to confirm the resistance of these plants against Anticarsia gemmatalis. Eleven progenies expressing cry1Ac, hpt and gusA genes were evaluated for agronomic characteristics in relation to the nontransformed parent IAS 5 cultivar. Cytogenetical analysis was carried out on transgenic and nontransgenic plants. Two out of the 11 transgenic progenies were also evaluated, in vitro and in vivo, for resistance to A. gemmatalis. Two negative controls were used in resistance bioassays: a transgenic homozygous line, containing only the gusA reporter gene, and nontransgenic 'IAS 5' plants. The presence of cry1Ac transgene affected neither the development nor the yield of plants. Cytogenetical analysis showed that transgenic plants presented normal karyotype. In detached-leaf bioassay, cry1Ac plants exhibited complete efficacy against A. gemmatalis, whereas negative controls were significantly damaged. Whole-plant feeding assay confirmed a very high protection of cry1Ac against velvetbean caterpillar, while nontransgenic 'IAS 5' plants and homozygous gusA line exhibited 56.5 and 71.5% defoliation, respectively. The presence of cry1Ac transgene doesn't affect the majority of agronomic traits (including yield) of soybean and grants high protection against A. gemmatalis.
650 $agene transfer
650 $aAnticarsia Gemmatalis
650 $aGlycine Max
650 $aSoja
653 $aplant resistance to insects
653 $aresistência de plantas a insetos
653 $atransferência de genes
700 1 $aPASSAGLIA, L. M. P.
700 1 $aPEREIRA, J. F.
700 1 $aBERTAGNOLLI, P. F.
700 1 $aSALVADORI, J. R.
700 1 $aNICOLAU, M.
700 1 $aKALTCHUK-SANTOS, E.
700 1 $aALVES, L. B.
700 1 $aBODANESE-ZANETTINI, M. H.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 43, n. 7, p. 801-807, jul. 2008.
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