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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
18/05/2023 |
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Autoria: |
ROSS, D. J. |
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Título: |
Influence of methods of determination, and time of day, on contents of nonstructural carbohydrates in grazed ryegrass-clover herbage from a nitrogen-fertilised pasture. |
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Ano de publicação: |
1978 |
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Fonte/Imprenta: |
New Zealand Journal of Agricultural Research, v. 21, n. 2, p. 223-229, 1978. |
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DOI: |
10.1080/00288233.1978.10427403 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Different methods for estimating contents of nonstructura1 carbohydrates in grazed ryegrass-white clover herbage from a pasture fertilised with nitrogen were investigated in some detail and compared; with all methods, a Somogyi copper reagent was used for the final titration of reducing sugars. Extraction with cold water, and then hot water, and subsequent hydrolysis of the extracts with dilute H2S04 gave results that generally agreed with those obtained with a method using an initial extraction with aqueous ethanol, and one using enzymatic hydrolysis with amy10g1ucosidase. The amount of carbohydrate soluble in hot water, Lut insoluble in cold water, was taken as an indication of the content of starch; other methods tor determining starch were also investigated. Some advantages and limitations of the above procedures are discussed. The water-extraction method was subsequently adopted for routine use in a more comprehensive investigation of the influence of nitrogen fertiliser on the chemical components of grass-clover herbage, The levels of total nonstructural carbohydrates in all of the samples of grass-clover herbage were low. In a time-of-day experiment, samples taken in mid morning had less (9-11 %) total water-soluble carbohydrates than those taken in rather late morning (11-13%) or mid afternoon (11-14%); the fraction soluble in hot water, but insoluble in cold water, corresponded to 0.6-0.8%, 0.7-0.9%, and 0.9-1.1 % respectively |
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Palavras-Chave: |
Fertilizacao; Metodo de determinacao. |
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Thesagro: |
Pastagem. |
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Thesaurus Nal: |
Carbohydrates; Lolium; Nitrogen fertilizers; Pastures. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02218naa a2200217 a 4500
001 1520183
005 2023-05-18
008 1978 bl uuuu u00u1 u #d
024 7 $a10.1080/00288233.1978.10427403$2DOI
100 1 $aROSS, D. J.
245 $aInfluence of methods of determination, and time of day, on contents of nonstructural carbohydrates in grazed ryegrass-clover herbage from a nitrogen-fertilised pasture.$h[electronic resource]
260 $c1978
520 $aAbstract: Different methods for estimating contents of nonstructura1 carbohydrates in grazed ryegrass-white clover herbage from a pasture fertilised with nitrogen were investigated in some detail and compared; with all methods, a Somogyi copper reagent was used for the final titration of reducing sugars. Extraction with cold water, and then hot water, and subsequent hydrolysis of the extracts with dilute H2S04 gave results that generally agreed with those obtained with a method using an initial extraction with aqueous ethanol, and one using enzymatic hydrolysis with amy10g1ucosidase. The amount of carbohydrate soluble in hot water, Lut insoluble in cold water, was taken as an indication of the content of starch; other methods tor determining starch were also investigated. Some advantages and limitations of the above procedures are discussed. The water-extraction method was subsequently adopted for routine use in a more comprehensive investigation of the influence of nitrogen fertiliser on the chemical components of grass-clover herbage, The levels of total nonstructural carbohydrates in all of the samples of grass-clover herbage were low. In a time-of-day experiment, samples taken in mid morning had less (9-11 %) total water-soluble carbohydrates than those taken in rather late morning (11-13%) or mid afternoon (11-14%); the fraction soluble in hot water, but insoluble in cold water, corresponded to 0.6-0.8%, 0.7-0.9%, and 0.9-1.1 % respectively
650 $aCarbohydrates
650 $aLolium
650 $aNitrogen fertilizers
650 $aPastures
650 $aPastagem
653 $aFertilizacao
653 $aMetodo de determinacao
773 $tNew Zealand Journal of Agricultural Research$gv. 21, n. 2, p. 223-229, 1978.
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