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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
27/10/2004 |
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Data da última atualização: |
04/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CASTRO, C. S. de; SILVESTRE. F. G.; ARAUJO, S. C.; YASBECK, G. de M.; MANGILI, O. C.; CRUZ, I.; CHAVEZ-OLORTEGUI, C.; KALAPOTHAKIS, E. |
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Afiliação: |
IVAN CRUZ, CNPMS. |
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Título: |
Identification and molecular cloning of insecticidal toxins from the venom of the brown spider Laxosceles intermedia. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Toxicon, Elmsford, v. 44, p. 273-280, 2004. |
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Idioma: |
Inglês |
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Conteúdo: |
The venom of Loxosceles intermedia was investigated for the presence of insecticidal toxins active against Spodoptera frugiperda (Lepidoptera: Noctuidade), an insect that has caused great reductions in corn production in Brazil. A combination of gel filtration (Sephadex G-100) and ion-exchange chromatography (Carboxymethyl Cellulose, CM 52) resulted in four major fractions that were submitted to biological assay. Fraction 4 was further purified by a reverse phase HPLC (C18 Column) resulting in peptides active against Spodoptera frugiperda. Three new potential insecticidal toxins named LiTx×1, LiTx×2 and LiTx×3 were identified. The partial amino terminal sequences of these peptides were obtained and used to clone the corresponding cDNAs with the help of degenerate oligonucleotides. The amino acid sequence deduced from the cDNA of LiTx×1, LiTx×2 and LiTx×3 revealed mature proteins of approximately 7.4, 7.9 and 5.6 kDa. |
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Palavras-Chave: |
cDNA; Insecticidal toxins; LiTx×1; LiTx×2; LiTx×3; Loxosceles intermedia; Spider; Toxin; Venom. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 01803naa a2200313 a 4500
001 1488354
005 2018-06-04
008 2004 bl uuuu u00u1 u #d
100 1 $aCASTRO, C. S. de
245 $aIdentification and molecular cloning of insecticidal toxins from the venom of the brown spider Laxosceles intermedia.$h[electronic resource]
260 $c2004
520 $aThe venom of Loxosceles intermedia was investigated for the presence of insecticidal toxins active against Spodoptera frugiperda (Lepidoptera: Noctuidade), an insect that has caused great reductions in corn production in Brazil. A combination of gel filtration (Sephadex G-100) and ion-exchange chromatography (Carboxymethyl Cellulose, CM 52) resulted in four major fractions that were submitted to biological assay. Fraction 4 was further purified by a reverse phase HPLC (C18 Column) resulting in peptides active against Spodoptera frugiperda. Three new potential insecticidal toxins named LiTx×1, LiTx×2 and LiTx×3 were identified. The partial amino terminal sequences of these peptides were obtained and used to clone the corresponding cDNAs with the help of degenerate oligonucleotides. The amino acid sequence deduced from the cDNA of LiTx×1, LiTx×2 and LiTx×3 revealed mature proteins of approximately 7.4, 7.9 and 5.6 kDa.
653 $acDNA
653 $aInsecticidal toxins
653 $aLiTx×1
653 $aLiTx×2
653 $aLiTx×3
653 $aLoxosceles intermedia
653 $aSpider
653 $aToxin
653 $aVenom
700 1 $aSILVESTRE. F. G.
700 1 $aARAUJO, S. C.
700 1 $aYASBECK, G. de M.
700 1 $aMANGILI, O. C.
700 1 $aCRUZ, I.
700 1 $aCHAVEZ-OLORTEGUI, C.
700 1 $aKALAPOTHAKIS, E.
773 $tToxicon, Elmsford$gv. 44, p. 273-280, 2004.
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