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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
29/06/1998 |
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Data da última atualização: |
12/06/2018 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
LOPES, M. A.; LARKINS, B. A. |
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Afiliação: |
EMBRAPA-CNPMS. |
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Título: |
Genetic analysis of endosperm modification in quality protein maize. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON QUALITY PROTEIN MAIZE, 1994, Sete Lagoas. Quality protein maize: 1964-1994: proceedings. [S.l.]: Purdue University, 1997. p. 149-173. |
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Idioma: |
Inglês |
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Conteúdo: |
A combination of genetic and biochemical analyses were used to investigate the number and mode of action of opaque-2 (o2) modifier genes and possible associations between seed modification and endosperm protein accumulation. Genetic analysis indicated the existence of two additive modifier genes in the populations studied. Analysis of 02, modified 02, their reciprocal F1's, and segregating progenies indicated that increased deposition of gamma zein, a cysteine-rich storage protein that contains no lysine, is dependent on the dosage of 02 modifier genes and directly correlated to seed modification. Molecular mapping by RFLP and Bulked Segregant Analyisis (BSA) indicated that a modifier gene maps to the same chromosomal region where gamma-zein genes are located. Analysis of recombination inbred lines with variable degrees of seed modification showed that allelic composition at the gamma-zein locus correlated with variations in endosperm modification. When we consider the possible involvement of gamma-zein in QPM endosperm modification, it is difficult to imagine that selection for increased seed modification would cause increased accumulation of a lysine-poor protein unrelated to the modification process, thus antagonizing efforts to maintain high nutritional quality. This is especially true because the selection procedures used for o2 modification placed a high priority on maintenance of seed nutritional quality. Therefore, it appears that increased accumulation of the lysine-poor gamma-zein protein was a necessary route to alter seed phenotype. Apparently, the cost of this process was a slight reduction of seed nutritional quality relative to the unmodified 02, due to increased gamma-zein accumulation. Although non-zein (lysine-containing proteins) content was found to be negatively correlated with seed density, amount of gamma-zein, and amount of total zein in the endosperm, we found that it is possible to select QPM lines with high levels of seed modification and high non-zein content. These results indicate the potential for further improvements in QPM nutritional quality. MenosA combination of genetic and biochemical analyses were used to investigate the number and mode of action of opaque-2 (o2) modifier genes and possible associations between seed modification and endosperm protein accumulation. Genetic analysis indicated the existence of two additive modifier genes in the populations studied. Analysis of 02, modified 02, their reciprocal F1's, and segregating progenies indicated that increased deposition of gamma zein, a cysteine-rich storage protein that contains no lysine, is dependent on the dosage of 02 modifier genes and directly correlated to seed modification. Molecular mapping by RFLP and Bulked Segregant Analyisis (BSA) indicated that a modifier gene maps to the same chromosomal region where gamma-zein genes are located. Analysis of recombination inbred lines with variable degrees of seed modification showed that allelic composition at the gamma-zein locus correlated with variations in endosperm modification. When we consider the possible involvement of gamma-zein in QPM endosperm modification, it is difficult to imagine that selection for increased seed modification would cause increased accumulation of a lysine-poor protein unrelated to the modification process, thus antagonizing efforts to maintain high nutritional quality. This is especially true because the selection procedures used for o2 modification placed a high priority on maintenance of seed nutritional quality. Therefore, it appears that increased accumulation of the lysine... Mostrar Tudo |
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Palavras-Chave: |
Maize; Protein; Quality. |
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Thesagro: |
Biologia Molecular; Milho; Qualidade; Zea Mays. |
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Thesaurus Nal: |
molecular biology. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02818nam a2200217 a 4500
001 1479111
005 2018-06-12
008 1997 bl uuuu u00u1 u #d
100 1 $aLOPES, M. A.
245 $aGenetic analysis of endosperm modification in quality protein maize.$h[electronic resource]
260 $aIn: INTERNATIONAL SYMPOSIUM ON QUALITY PROTEIN MAIZE, 1994, Sete Lagoas. Quality protein maize: 1964-1994: proceedings. [S.l.]: Purdue University, 1997. p. 149-173.$c1997
520 $aA combination of genetic and biochemical analyses were used to investigate the number and mode of action of opaque-2 (o2) modifier genes and possible associations between seed modification and endosperm protein accumulation. Genetic analysis indicated the existence of two additive modifier genes in the populations studied. Analysis of 02, modified 02, their reciprocal F1's, and segregating progenies indicated that increased deposition of gamma zein, a cysteine-rich storage protein that contains no lysine, is dependent on the dosage of 02 modifier genes and directly correlated to seed modification. Molecular mapping by RFLP and Bulked Segregant Analyisis (BSA) indicated that a modifier gene maps to the same chromosomal region where gamma-zein genes are located. Analysis of recombination inbred lines with variable degrees of seed modification showed that allelic composition at the gamma-zein locus correlated with variations in endosperm modification. When we consider the possible involvement of gamma-zein in QPM endosperm modification, it is difficult to imagine that selection for increased seed modification would cause increased accumulation of a lysine-poor protein unrelated to the modification process, thus antagonizing efforts to maintain high nutritional quality. This is especially true because the selection procedures used for o2 modification placed a high priority on maintenance of seed nutritional quality. Therefore, it appears that increased accumulation of the lysine-poor gamma-zein protein was a necessary route to alter seed phenotype. Apparently, the cost of this process was a slight reduction of seed nutritional quality relative to the unmodified 02, due to increased gamma-zein accumulation. Although non-zein (lysine-containing proteins) content was found to be negatively correlated with seed density, amount of gamma-zein, and amount of total zein in the endosperm, we found that it is possible to select QPM lines with high levels of seed modification and high non-zein content. These results indicate the potential for further improvements in QPM nutritional quality.
650 $amolecular biology
650 $aBiologia Molecular
650 $aMilho
650 $aQualidade
650 $aZea Mays
653 $aMaize
653 $aProtein
653 $aQuality
700 1 $aLARKINS, B. A.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
26/12/2017 |
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Data da última atualização: |
09/04/2024 |
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Tipo da produção científica: |
Artigo de Divulgação na Mídia |
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Autoria: |
CARVALHO, G. R.; ROCHA, D. T. da; RESENDE, J. C. de; LEITE, J. L. B.; STOCK, L. A.; HOTT, M. C.; ANDRADE, R. G.; TEIXEIRA, S. R.; MOTA, J.; TOSTES, V.; MACEDO, V. |
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Afiliação: |
GLAUCO RODRIGUES CARVALHO, CNPGL; DENIS TEIXEIRA DA ROCHA, CNPGL; JOAO CESAR DE RESENDE, CNPGL; JOSE LUIZ BELLINI LEITE, CNPGL; LORILDO ALDO STOCK, CNPGL; MARCOS CICARINI HOTT, CNPGL; RICARDO GUIMARAES ANDRADE, CNPGL; SERGIO RUSTICHELLI TEIXEIRA, CNPGL; Juliana Mota, UFJF; Vinicius Tostes, UFJF; Vinícius Macedo, UFJF. |
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Título: |
Leite e derivados: agosto/2017. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Intelactus, ago. 2017. |
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Idioma: |
Português |
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Palavras-Chave: |
Exportador de lácteos; Leite em pó intergral; UHT. |
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Categoria do assunto: |
E Economia e Indústria Agrícola |
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Marc: |
LEADER 00665nam a2200253 a 4500
001 2083510
005 2024-04-09
008 2017 bl uuuu u00u1 u #d
100 1 $aCARVALHO, G. R.
245 $aLeite e derivados$bagosto/2017.$h[electronic resource]
260 $aIntelactus, ago. 2017.$c2017
653 $aExportador de lácteos
653 $aLeite em pó intergral
653 $aUHT
700 1 $aROCHA, D. T. da
700 1 $aRESENDE, J. C. de
700 1 $aLEITE, J. L. B.
700 1 $aSTOCK, L. A.
700 1 $aHOTT, M. C.
700 1 $aANDRADE, R. G.
700 1 $aTEIXEIRA, S. R.
700 1 $aMOTA, J.
700 1 $aTOSTES, V.
700 1 $aMACEDO, V.
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