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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
23/01/2006 |
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Data da última atualização: |
08/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BICUDO, T. C.; FORATO, L. A.; BATISTA, L. A. R.; COLNAGO, L. A. |
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Afiliação: |
TATIANA C. BICUDO; LUCIMARA A. FORATO; LUIZ ALBERTO ROCHA BATISTA, CPPSE; LUIZ ALBERTO COLNAGO, CNPDIA. |
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Título: |
Study of the conformation of Y-zeins in purifield maize protein bodies by FTIR and NMR spectroscopy. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Analytical and Bioanlytical Chemistry, v. 383, p. 291-296, 2005. |
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DOI: |
10.1007/s00216-005-0003-z |
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Idioma: |
Inglês |
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Conteúdo: |
The gamma-zeins are a mixture of 16, 27, and 50-kDa polypeptides which are important in the formation and stabilization of protein bodies (PB). These organelles are used for deposition of zeins, the water-insoluble storage proteins in maize. The nature of the physical interaction between proteins in the assembly and stabilization of PB are fairly well known. It is suggested the repeated hexapeptide sequence (PPPVHL)(8) in the N-terminus is responsible for aggregation of the gamma-zeins on the PB surface. Despite this importance, there is little information about the native conformation of gamma-zeins. In this work, we have analyzed the secondary structures of gamma-zeins in purified protein bodies from two maize cultivars, in the solid state, by FTIR and NMR spectroscopy. The results revealed that gamma-zeins in their physiological state are comprise similar proportions of alpha-helix and beta-sheet, 33 and 31% as determined by FTIR. It was not possible to state if the polyproline II (PPII) conformation is present in the solid-state structure of gamma-zeins, as has been demonstrated for the hexapeptide in solution. Because of the similarity of the solid-state NMR spectra of gamma and alpha-zeins in the alpha carbon region we attributed their contributions to the beta-sheet structures rather than to the PPII conformation or a mixture of these extended structures. |
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Palavras-Chave: |
FTIR; Solid state NMR; Y Zeins. |
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Thesaurus Nal: |
Protein bodies; Protein secondary structure. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02089naa a2200229 a 4500
001 1047274
005 2023-05-08
008 2005 bl uuuu u00u1 u #d
024 7 $a10.1007/s00216-005-0003-z$2DOI
100 1 $aBICUDO, T. C.
245 $aStudy of the conformation of Y-zeins in purifield maize protein bodies by FTIR and NMR spectroscopy.$h[electronic resource]
260 $c2005
520 $aThe gamma-zeins are a mixture of 16, 27, and 50-kDa polypeptides which are important in the formation and stabilization of protein bodies (PB). These organelles are used for deposition of zeins, the water-insoluble storage proteins in maize. The nature of the physical interaction between proteins in the assembly and stabilization of PB are fairly well known. It is suggested the repeated hexapeptide sequence (PPPVHL)(8) in the N-terminus is responsible for aggregation of the gamma-zeins on the PB surface. Despite this importance, there is little information about the native conformation of gamma-zeins. In this work, we have analyzed the secondary structures of gamma-zeins in purified protein bodies from two maize cultivars, in the solid state, by FTIR and NMR spectroscopy. The results revealed that gamma-zeins in their physiological state are comprise similar proportions of alpha-helix and beta-sheet, 33 and 31% as determined by FTIR. It was not possible to state if the polyproline II (PPII) conformation is present in the solid-state structure of gamma-zeins, as has been demonstrated for the hexapeptide in solution. Because of the similarity of the solid-state NMR spectra of gamma and alpha-zeins in the alpha carbon region we attributed their contributions to the beta-sheet structures rather than to the PPII conformation or a mixture of these extended structures.
650 $aProtein bodies
650 $aProtein secondary structure
653 $aFTIR
653 $aSolid state NMR
653 $aY Zeins
700 1 $aFORATO, L. A.
700 1 $aBATISTA, L. A. R.
700 1 $aCOLNAGO, L. A.
773 $tAnalytical and Bioanlytical Chemistry$gv. 383, p. 291-296, 2005.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
13/08/2023 |
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Data da última atualização: |
13/08/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PEREIRA, D. G. C.; PORTUGAL, A. F.; GIUSTOLIN, T. A.; MAIA, V. M.; MEGDA, M. X. V.; KONDO, M. K. |
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Afiliação: |
DAVID GABRIEL CAMPOS PEREIRA, Universidade Estadual de Montes Claros; ARLEY FIGUEIREDO PORTUGAL, CNPMS; TERESINHA AUGUSTA GIUSTOLIN, Universidade Estadual de Montes Claros; VICTOR MARTINS MAIA, Universidade Estadual de Montes Claros; MICHELE XAVIER VIEIRA MEGDA, Universidade Estadual de Montes Claros; MARCOS KOITI KONDO, Universidade Estadual de Montes Claros. |
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Título: |
Litter decomposition and nutrient release in different land use systems in the Brazilian semi-arid region. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Catena, v. 231, 107345, 2023. |
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DOI: |
https://doi.org/10.1016/j.catena.2023.107345 |
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Idioma: |
Inglês |
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Conteúdo: |
Climate and litter quality change the dynamics of soil organic matter (SOM) in agricultural systems, directly influencing the soil biological activity and, consequently, energy and nutrient cycling. This work evaluated the dynamics of organic carbon decomposition and nutrient release in litter in three land use management systems. The study was carried out under semi-arid conditions in Minas Gerais State, Brazil, using decomposition bags arranged in a randomized block design and four replications. The treatments consisted of three land use systems (native forest, corn, and cocoa) and seven evaluation times: 0, 30, 90, 150, 210, 270, and 330 days. Was evaluated dry matter production, litter decomposition in polyamide bags, and nutrient release (N, P, K, S, Ca, and Mg). Systems with native forest, cocoa and maize had similar average monthly litter production, a fact linked to the climate conditions. While in the semi-arid climate, the full-sun cocoa system showed a sudden oscillation in relation to the organic carbon content up to 330 days, the native forest and corn systems were more balanced, whitout significant changes. The higher litter C/N ratio in relation to the native and corn systems favors the maintenance of organic C levels in the soil. K release has a similar behavior in cultivated soils and occurs more quickly when compared to the native system. Regarding the other macronutrients, the soil in the cocoa production system showed the highest mineralization. Periods of accumulated precipitation accelerate litter decomposition and macronutrient release. The results of this study give more insight into the carbon dynamics and nutrient release from litter as a function of land use systems in a semi-arid region. MenosClimate and litter quality change the dynamics of soil organic matter (SOM) in agricultural systems, directly influencing the soil biological activity and, consequently, energy and nutrient cycling. This work evaluated the dynamics of organic carbon decomposition and nutrient release in litter in three land use management systems. The study was carried out under semi-arid conditions in Minas Gerais State, Brazil, using decomposition bags arranged in a randomized block design and four replications. The treatments consisted of three land use systems (native forest, corn, and cocoa) and seven evaluation times: 0, 30, 90, 150, 210, 270, and 330 days. Was evaluated dry matter production, litter decomposition in polyamide bags, and nutrient release (N, P, K, S, Ca, and Mg). Systems with native forest, cocoa and maize had similar average monthly litter production, a fact linked to the climate conditions. While in the semi-arid climate, the full-sun cocoa system showed a sudden oscillation in relation to the organic carbon content up to 330 days, the native forest and corn systems were more balanced, whitout significant changes. The higher litter C/N ratio in relation to the native and corn systems favors the maintenance of organic C levels in the soil. K release has a similar behavior in cultivated soils and occurs more quickly when compared to the native system. Regarding the other macronutrients, the soil in the cocoa production system showed the highest mineralization. Periods o... Mostrar Tudo |
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Palavras-Chave: |
Saco de lixo. |
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Thesagro: |
Cacau; Floresta Nativa; Milho; Serapilheira; Uso da Terra. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02513naa a2200265 a 4500
001 2155879
005 2023-08-13
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.catena.2023.107345$2DOI
100 1 $aPEREIRA, D. G. C.
245 $aLitter decomposition and nutrient release in different land use systems in the Brazilian semi-arid region.$h[electronic resource]
260 $c2023
520 $aClimate and litter quality change the dynamics of soil organic matter (SOM) in agricultural systems, directly influencing the soil biological activity and, consequently, energy and nutrient cycling. This work evaluated the dynamics of organic carbon decomposition and nutrient release in litter in three land use management systems. The study was carried out under semi-arid conditions in Minas Gerais State, Brazil, using decomposition bags arranged in a randomized block design and four replications. The treatments consisted of three land use systems (native forest, corn, and cocoa) and seven evaluation times: 0, 30, 90, 150, 210, 270, and 330 days. Was evaluated dry matter production, litter decomposition in polyamide bags, and nutrient release (N, P, K, S, Ca, and Mg). Systems with native forest, cocoa and maize had similar average monthly litter production, a fact linked to the climate conditions. While in the semi-arid climate, the full-sun cocoa system showed a sudden oscillation in relation to the organic carbon content up to 330 days, the native forest and corn systems were more balanced, whitout significant changes. The higher litter C/N ratio in relation to the native and corn systems favors the maintenance of organic C levels in the soil. K release has a similar behavior in cultivated soils and occurs more quickly when compared to the native system. Regarding the other macronutrients, the soil in the cocoa production system showed the highest mineralization. Periods of accumulated precipitation accelerate litter decomposition and macronutrient release. The results of this study give more insight into the carbon dynamics and nutrient release from litter as a function of land use systems in a semi-arid region.
650 $aCacau
650 $aFloresta Nativa
650 $aMilho
650 $aSerapilheira
650 $aUso da Terra
653 $aSaco de lixo
700 1 $aPORTUGAL, A. F.
700 1 $aGIUSTOLIN, T. A.
700 1 $aMAIA, V. M.
700 1 $aMEGDA, M. X. V.
700 1 $aKONDO, M. K.
773 $tCatena$gv. 231, 107345, 2023.
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